6zn7 - Revision history

OCA at 11:54, 1 February 2024

OCA — Thu, 01 Feb 2024 11:54:55 GMT

←Older revision		Revision as of 11:54, 1 February 2024
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	<StructureSection load='6zn7' size='340' side='right'caption='[[6zn7]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='6zn7' size='340' side='right'caption='[[6zn7]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6zn7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bdeba Bdeba~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zn7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bdellovibrio_bacteriovorus_HD100 Bdellovibrio bacteriovorus HD100]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[6zn4\|6zn4]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">mdh, Bd1833 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264462 BDEBA])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn7 OCA], [https://pdbe.org/6zn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn7 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn7 OCA], [https://pdbe.org/6zn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn7 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6MM15_BDEBA Q6MM15_BDEBA] [https://www.uniprot.org/uniprot/Q6MM14_BDEBA Q6MM14_BDEBA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6zn7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6zn7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Malate Dehydrogenase 3D structures\|Malate Dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bdeba~~]]	+	[[Category: Bdellovibrio bacteriovorus HD100]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Harding~~, C J~~]]	+	[[Category: Harding CJ]]
-	[[Category: Lovering~~, A L]]~~	+	[[Category: Lovering AL]]
-	~~[[Category: Malic enzyme]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:55, 17 March 2021

OCA — Wed, 17 Mar 2021 06:55:39 GMT

←Older revision		Revision as of 06:55, 17 March 2021
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	==MaeB malic enzyme domain apoprotein==		==MaeB malic enzyme domain apoprotein==
-	<StructureSection load='6zn7' size='340' side='right'caption='[[6zn7]]' scene=''>	+	<StructureSection load='6zn7' size='340' side='right'caption='[[6zn7]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zn7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bdeba Bdeba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn7 OCA], [https://pdbe.org/6zn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn7 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn7 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6zn4\|6zn4]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mdh, Bd1833 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264462 BDEBA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn7 OCA], [https://pdbe.org/6zn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn7 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial hybrid malic enzymes (MaeB grouping, multidomain) catalyse the transformation of malate to pyruvate, and are a major contributor to cellular reducing power and carbon flux. Distinct from other malic enzyme subtypes, the hybrid enzymes are regulated by acetyl-CoA, a molecular indicator of the metabolic state of the cell. Here we solve the structure of a MaeB protein, which reveals hybrid enzymes use the appended phosphotransacetylase (PTA) domain to form a hexameric sensor that communicates acetyl-CoA occupancy to the malic enzyme active site, 60 A away. We demonstrate that allostery is governed by a large-scale rearrangement that rotates the catalytic subunits 70 degrees between the two states, identifying MaeB as a new model enzyme for the study of ligand-induced conformational change. Our work provides the mechanistic basis for metabolic control of hybrid malic enzymes, and identifies inhibition-insensitive variants that may find utility in synthetic biology.
		+
		+	A rotary mechanism for allostery in bacterial hybrid malic enzymes.,Harding CJ, Cadby IT, Moynihan PJ, Lovering AL Nat Commun. 2021 Feb 23;12(1):1228. doi: 10.1038/s41467-021-21528-2. PMID:33623032<ref>PMID:33623032</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zn7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bdeba]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Harding CJ]]	+	[[Category: Harding, C J]]
-	[[Category: Lovering AL]]	+	[[Category: Lovering, A L]]
		+	[[Category: Malic enzyme]]
		+	[[Category: Oxidoreductase]]

OCA at 09:59, 17 February 2021

OCA — Wed, 17 Feb 2021 09:59:06 GMT

←Older revision		Revision as of 09:59, 17 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6zn7 ~~is ON HOLD until Paper Publication~~	+	==MaeB malic enzyme domain apoprotein==
-		+	<StructureSection load='6zn7' size='340' side='right'caption='[[6zn7]]' scene=''>
-	~~Authors~~: ~~Lovering~~, A.L., ~~Harding~~, C.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN7 FirstGlance]. <br>
-	~~Description: MaeB malic enzyme domain apoprotein~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn7 OCA], [https://pdbe.org/6zn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn7 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn7 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Harding~~, C.J~~]]	+	__TOC__
-	[[Category: Lovering~~, A.L~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Harding CJ]]
		+	[[Category: Lovering AL]]

OCA at 03:56, 5 August 2020

OCA — Wed, 05 Aug 2020 03:56:00 GMT

←Older revision		Revision as of 03:56, 5 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6zn7 is ON HOLD	+	The entry 6zn7 is ON HOLD until Paper Publication

	Authors: Lovering, A.L., Harding, C.J.		Authors: Lovering, A.L., Harding, C.J.

OCA: Protected "6zn7" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 08:03:25 GMT

Protected "6zn7" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:03, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zn7 is ON HOLD Authors: Lovering, A.L., Harding, C.J. Description: MaeB malic enzyme domain apoprotein Category: Unreleased Structures [[Categ...

OCA — Wed, 22 Jul 2020 08:03:23 GMT

New page: '''Unreleased structure''' The entry 6zn7 is ON HOLD Authors: Lovering, A.L., Harding, C.J. Description: MaeB malic enzyme domain apoprotein Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 6zn7 is ON HOLD

Authors: Lovering, A.L., Harding, C.J.

Description: MaeB malic enzyme domain apoprotein
[[Category: Unreleased Structures]]
[[Category: Harding, C.J]]
[[Category: Lovering, A.L]]