OCA at 10:17, 19 May 2021

OCA — Wed, 19 May 2021 10:17:24 GMT

←Older revision		Revision as of 10:17, 19 May 2021
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	==Crystal structure of the unconventional kinetochore protein Leishmania mexicana KKT4 coiled coil domain==		==Crystal structure of the unconventional kinetochore protein Leishmania mexicana KKT4 coiled coil domain==
-	<StructureSection load='6zpj' size='340' side='right'caption='[[6zpj]]' scene=''>	+	<StructureSection load='6zpj' size='340' side='right'caption='[[6zpj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZPJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6ZPJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zpj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leimu Leimu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZPJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6zpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zpj OCA], [~~http~~://pdbe.org/6zpj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6zpj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6zpj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6zpj ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LMXM_10_0300 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=929439 LEIMU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zpj OCA], [https://pdbe.org/6zpj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zpj RCSB], [https://www.ebi.ac.uk/pdbsum/6zpj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zpj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The kinetochore is the macromolecular machinery that drives chromosome segregation by interacting with spindle microtubules. Kinetoplastids (such as Trypanosoma brucei), a group of evolutionarily divergent eukaryotes, have a unique set of kinetochore proteins that lack any significant homology to canonical kinetochore components. To date, KKT4 is the only kinetoplastid kinetochore protein that is known to bind microtubules. Here we use X-ray crystallography, NMR spectroscopy, and crosslinking mass spectrometry to characterize the structure and dynamics of KKT4. We show that its microtubule-binding domain consists of a coiled-coil structure followed by a positively charged disordered tail. The structure of the C-terminal BRCT domain of KKT4 reveals that it is likely a phosphorylation-dependent protein-protein interaction domain. The BRCT domain interacts with the N-terminal region of the KKT4 microtubule-binding domain and with a phosphopeptide derived from KKT8. Taken together, these results provide structural insights into the unconventional kinetoplastid kinetochore protein KKT4.
		+
		+	Structural characterization of KKT4, an unconventional microtubule-binding kinetochore protein.,Ludzia P, Lowe ED, Marciano G, Mohammed S, Redfield C, Akiyoshi B Structure. 2021 Apr 26. pii: S0969-2126(21)00120-9. doi:, 10.1016/j.str.2021.04.004. PMID:33915106<ref>PMID:33915106</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zpj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akiyoshi B]]	+	[[Category: Leimu]]
-	[[Category: Lowe ED]]	+	[[Category: Akiyoshi, B]]
-	[[Category: Ludzia P]]	+	[[Category: Lowe, E D]]
-	[[Category: Marciano G]]	+	[[Category: Ludzia, P]]
-	[[Category: Mohammed S]]	+	[[Category: Marciano, G]]
-	[[Category: Redfield C]]	+	[[Category: Mohammed, S]]
		+	[[Category: Redfield, C]]
		+	[[Category: Cell cycle]]
		+	[[Category: Coiled coil]]
		+	[[Category: Kinetochore]]
		+	[[Category: Kinetoplastid]]
		+	[[Category: Microtubule]]

OCA at 08:16, 21 October 2020

OCA — Wed, 21 Oct 2020 08:16:22 GMT

←Older revision		Revision as of 08:16, 21 October 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6zpj is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the unconventional kinetochore protein Leishmania mexicana KKT4 coiled coil domain==
-		+	<StructureSection load='6zpj' size='340' side='right'caption='[[6zpj]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZPJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZPJ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zpj OCA], [http://pdbe.org/6zpj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zpj RCSB], [http://www.ebi.ac.uk/pdbsum/6zpj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zpj ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Akiyoshi B]]
		+	[[Category: Lowe ED]]
		+	[[Category: Ludzia P]]
		+	[[Category: Marciano G]]
		+	[[Category: Mohammed S]]
		+	[[Category: Redfield C]]

OCA: Protected "6zpj" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 08:10:23 GMT

Protected "6zpj" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:10, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zpj is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Jul 2020 08:10:21 GMT

New page: '''Unreleased structure''' The entry 6zpj is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6zpj is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

6zpj - Revision history

OCA at 10:17, 19 May 2021

OCA at 08:16, 21 October 2020

OCA: Protected "6zpj" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6zpj is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures