OCA at 09:56, 18 November 2020

OCA — Wed, 18 Nov 2020 09:56:28 GMT

←Older revision		Revision as of 09:56, 18 November 2020
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	==Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3 Collected at 2.75 A==		==Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3 Collected at 2.75 A==
-	<StructureSection load='6zps' size='340' side='right'caption='[[6zps]]' scene=''>	+	<StructureSection load='6zps' size='340' side='right'caption='[[6zps]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZPS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZPS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zps]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Boletus_giganteus Boletus giganteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZPS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZPS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zps OCA], [http://pdbe.org/6zps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zps RCSB], [http://www.ebi.ac.uk/pdbsum/6zps PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zps ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-reducing_end_alpha-L-arabinofuranosidase Non-reducing end alpha-L-arabinofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zps OCA], [http://pdbe.org/6zps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zps RCSB], [http://www.ebi.ac.uk/pdbsum/6zps PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zps ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-L-Arabinofuranosidases from glycoside hydrolase family 51 use a stereochemically retaining hydrolytic mechanism to liberate nonreducing terminal alpha-L-arabinofuranose residues from plant polysaccharides such as arabinoxylan and arabinan. To date, more than ten fungal GH51 alpha-L-arabinofuranosidases have been functionally characterized, yet no structure of a fungal GH51 enzyme has been solved. In contrast, seven bacterial GH51 enzyme structures, with low sequence similarity to the fungal GH51 enzymes, have been determined. Here, the crystallization and structural characterization of MgGH51, an industrially relevant GH51 alpha-L-arabinofuranosidase cloned from Meripilus giganteus, are reported. Three crystal forms were grown in different crystallization conditions. The unliganded structure was solved using sulfur SAD data collected from a single crystal using the I23 in vacuo diffraction beamline at Diamond Light Source. Crystal soaks with arabinose, 1,4-dideoxy-1,4-imino-L-arabinitol and two cyclophellitol-derived arabinose mimics reveal a conserved catalytic site and conformational itinerary between fungal and bacterial GH51 alpha-L-arabinofuranosidases.
		+
		+	Structure of a GH51 alpha-L-arabinofuranosidase from Meripilus giganteus: conserved substrate recognition from bacteria to fungi.,McGregor NGS, Turkenburg JP, Morkeberg Krogh KBR, Nielsen JE, Artola M, Stubbs KA, Overkleeft HS, Davies GJ Acta Crystallogr D Struct Biol. 2020 Nov 1;76(Pt 11):1124-1133. doi:, 10.1107/S205979832001253X. Epub 2020 Oct 16. PMID:33135683<ref>PMID:33135683</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zps" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Boletus giganteus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Davies GJ]]	+	[[Category: Non-reducing end alpha-L-arabinofuranosidase]]
-	[[Category: McGregor ~~NGS~~]]	+	[[Category: Davies, G J]]
		+	[[Category: McGregor, N G.S]]
		+	[[Category: Arabinofuranosidse]]
		+	[[Category: Gh51]]
		+	[[Category: Glycosidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Sulphur sad]]

OCA at 08:01, 11 November 2020

OCA — Wed, 11 Nov 2020 08:01:24 GMT

←Older revision		Revision as of 08:01, 11 November 2020
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-	'''Unreleased structure'''

-	~~The entry 6zps is ON HOLD until Paper Publication~~	+	==Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3 Collected at 2.75 A==
-		+	<StructureSection load='6zps' size='340' side='right'caption='[[6zps]]' scene=''>
-	~~Authors: McGregor, N.G.S., Davies, G.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZPS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZPS FirstGlance]. <br>
-	~~Description:~~ Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3 Collected at 2.75 A	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zps OCA], [http://pdbe.org/6zps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zps RCSB], [http://www.ebi.ac.uk/pdbsum/6zps PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zps ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Mcgregor~~, N.G.S]]	+	__TOC__
-	[[Category: Davies~~, G.J~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Davies GJ]]
		+	[[Category: McGregor NGS]]

OCA: Protected "6zps" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 08:11:05 GMT

Protected "6zps" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:11, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zps is ON HOLD until Paper Publication Authors: McGregor, N.G.S., Davies, G.J. Description: Structure of Unliganded MgGH51 a-L-Arabinofuranosidase...

OCA — Wed, 22 Jul 2020 08:11:04 GMT

New page: '''Unreleased structure''' The entry 6zps is ON HOLD until Paper Publication Authors: McGregor, N.G.S., Davies, G.J. Description: Structure of Unliganded MgGH51 a-L-Arabinofuranosidase...

New page

'''Unreleased structure'''

The entry 6zps is ON HOLD until Paper Publication

Authors: McGregor, N.G.S., Davies, G.J.

Description: Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3 Collected at 2.75 A
[[Category: Unreleased Structures]]
[[Category: Mcgregor, N.G.S]]
[[Category: Davies, G.J]]

6zps - Revision history

OCA at 09:56, 18 November 2020

OCA at 08:01, 11 November 2020

OCA: Protected "6zps" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6zps is ON HOLD until Paper Publication Authors: McGregor, N.G.S., Davies, G.J. Description: Structure of Unliganded MgGH51 a-L-Arabinofuranosidase...