6zqs - Revision history

OCA at 11:56, 1 February 2024

OCA — Thu, 01 Feb 2024 11:56:31 GMT

←Older revision		Revision as of 11:56, 1 February 2024
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-	====	+	==Crystal structure of double-phosphorylated p38alpha with ATF2(83-102)==
-	<StructureSection load='6zqs' size='340' side='right'caption='[[6zqs]]' scene=''>	+	<StructureSection load='6zqs' size='340' side='right'caption='[[6zqs]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zqs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZQS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6zqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zqs OCA], [~~http~~://pdbe.org/6zqs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6zqs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6zqs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6zqs ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3FF:2-[(2,4-DIFLUOROPHENYL)AMINO]-7-{[(2R)-2,3-DIHYDROXYPROPYL]OXY}-10,11-DIHYDRO-5H-DIBENZO[A,D][7]ANNULEN-5-ONE'>3FF</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zqs OCA], [https://pdbe.org/6zqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zqs RCSB], [https://www.ebi.ac.uk/pdbsum/6zqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zqs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ATF2_HUMAN ATF2_HUMAN] Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transcription factor phosphorylation at specific sites often activates gene expression, but how environmental cues quantitatively control transcription is not well-understood. Activating protein 1 transcription factors are phosphorylated by mitogen-activated protein kinases (MAPK) in their transactivation domains (TAD) at so-called phosphoswitches, which are a hallmark in response to growth factors, cytokines or stress. We show that the ATF2 TAD is controlled by functionally distinct signaling pathways (JNK and p38) through structurally different MAPK binding sites. Moreover, JNK mediated phosphorylation at an evolutionarily more recent site diminishes p38 binding and made the phosphoswitch differently sensitive to JNK and p38 in vertebrates. Structures of MAPK-TAD complexes and mechanistic modeling of ATF2 TAD phosphorylation in cells suggest that kinase binding motifs and phosphorylation sites line up to maximize MAPK based co-regulation. This study shows how the activity of an ancient transcription controlling phosphoswitch became dependent on the relative flux of upstream signals.
		+
		+	Co-regulation of the transcription controlling ATF2 phosphoswitch by JNK and p38.,Kirsch K, Zeke A, Toke O, Sok P, Sethi A, Sebo A, Kumar GS, Egri P, Poti AL, Gooley P, Peti W, Bento I, Alexa A, Remenyi A Nat Commun. 2020 Nov 13;11(1):5769. doi: 10.1038/s41467-020-19582-3. PMID:33188182<ref>PMID:33188182</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zqs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Mitogen-activated protein kinase 3D structures\|Mitogen-activated protein kinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Kirsch K]]
		+	[[Category: Poti AL]]
		+	[[Category: Remenyi A]]
		+	[[Category: Sok P]]

OCA at 09:39, 18 November 2020

OCA — Wed, 18 Nov 2020 09:39:39 GMT

←Older revision		Revision as of 09:39, 18 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6zqs ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6zqs' size='340' side='right'caption='[[6zqs]]' scene=''>
-	~~Authors~~: ~~Kirsch, K~~.~~, Sok, P~~.~~, Poti, A~~.L.~~, Remenyi, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of double-phosphorylated p38alpha with ATF2(83-102)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zqs OCA], [http://pdbe.org/6zqs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zqs RCSB], [http://www.ebi.ac.uk/pdbsum/6zqs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zqs ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Kirsch, K~~]]	+	__TOC__
-	[~~[Category~~: ~~Remenyi, A~~]]	+	</StructureSection>
-	[[Category: ~~Poti, A.L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sok, P~~]]	+	[[Category: Z-disk]]

OCA at 08:11, 12 August 2020

OCA — Wed, 12 Aug 2020 08:11:42 GMT

←Older revision		Revision as of 08:11, 12 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6zqs is ON HOLD	+	The entry 6zqs is ON HOLD until Paper Publication

	Authors: Kirsch, K., Sok, P., Poti, A.L., Remenyi, A.		Authors: Kirsch, K., Sok, P., Poti, A.L., Remenyi, A.

OCA: Protected "6zqs" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 08:14:12 GMT

Protected "6zqs" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:14, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zqs is ON HOLD Authors: Kirsch, K., Sok, P., Poti, A.L., Remenyi, A. Description: Crystal structure of double-phosphorylated p38alpha with ATF2(83...

OCA — Wed, 22 Jul 2020 08:14:10 GMT

New page: '''Unreleased structure''' The entry 6zqs is ON HOLD Authors: Kirsch, K., Sok, P., Poti, A.L., Remenyi, A. Description: Crystal structure of double-phosphorylated p38alpha with ATF2(83...

New page

'''Unreleased structure'''

The entry 6zqs is ON HOLD

Authors: Kirsch, K., Sok, P., Poti, A.L., Remenyi, A.

Description: Crystal structure of double-phosphorylated p38alpha with ATF2(83-102)
[[Category: Unreleased Structures]]
[[Category: Kirsch, K]]
[[Category: Remenyi, A]]
[[Category: Poti, A.L]]
[[Category: Sok, P]]