6zry - Revision history

OCA at 11:57, 1 February 2024

OCA — Thu, 01 Feb 2024 11:57:20 GMT

←Older revision		Revision as of 11:57, 1 February 2024
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	<StructureSection load='6zry' size='340' side='right'caption='[[6zry]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='6zry' size='340' side='right'caption='[[6zry]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6zry]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_21819 Atcc 21819~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZRY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6ZRY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zry]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_olivasterospora Micromonospora olivasterospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZRY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZRY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.652Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[6zrx\|6zrx]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">MolI14.36, JD77_02062 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1880 ATCC 21819])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zry OCA], [https://pdbe.org/6zry PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zry RCSB], [https://www.ebi.ac.uk/pdbsum/6zry PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zry ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6zry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zry OCA], [~~http~~://pdbe.org/6zry PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6zry RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6zry PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6zry ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q2MFY2_MICOL Q2MFY2_MICOL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6zry" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6zry" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tryptophan synthase 3D structures\|Tryptophan synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 21819]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ostertag, E~~]]	+	[[Category: Micromonospora olivasterospora]]
-	[[Category: ~~Stehle, T~~]]	+	[[Category: Ostertag E]]
-	[[Category: ~~Zocher, G~~]]	+	[[Category: Stehle T]]
-	[[Category: ~~Abba-barrel fold]]~~	+	[[Category: Zocher G]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Prenyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:01, 30 December 2020

OCA — Wed, 30 Dec 2020 07:01:12 GMT

←Older revision		Revision as of 07:01, 30 December 2020
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	==6-dimethylallyl tryptophan synthase==		==6-dimethylallyl tryptophan synthase==
-	<StructureSection load='6zry' size='340' side='right'caption='[[6zry]]' scene=''>	+	<StructureSection load='6zry' size='340' side='right'caption='[[6zry]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZRY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZRY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zry]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_21819 Atcc 21819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZRY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZRY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zry OCA], [http://pdbe.org/6zry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zry RCSB], [http://www.ebi.ac.uk/pdbsum/6zry PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zry ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6zrx\|6zrx]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MolI14.36, JD77_02062 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1880 ATCC 21819])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zry OCA], [http://pdbe.org/6zry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zry RCSB], [http://www.ebi.ac.uk/pdbsum/6zry PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zry ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Prenylation is a process widely prevalent in primary and secondary metabolism, contributing to functionality and chemical diversity in natural systems. Due to their high regio- and chemoselectivities, prenyltransferases are also valuable tools for creation of new compounds by chemoenzymatic synthesis and synthetic biology. Over the last ten years, biochemical and structural investigations shed light on the mechanism and key residues that control the catalytic process, but to date crucial information on how certain prenyltransferases control regioselectivity and chemoselectivity was still lacking. Here, we advance a general understanding of the enzyme family by contributing the first structure of a tryptophan C5-prenyltransferase 5-DMATS. Additinallyi, the structure of a bacterial tryptophan C6-prenyltransferase 6-DMATS was solved. Analysis and comparison of both substrate-bound complexes led to the identification of key residues for catalysis. Next, site-directed mutagenesis was successfully implemented to not only modify the prenyl donor specificity but also to redirect the prenylation, thereby switching the regioselectivity of 6-DMATS to that of 5-DMATS. The general strategy of structure-guided protein engineering should be applicable to other related prenyltransferases, thus enabling the production of novel prenylated compounds.
		+
		+	Reprogramming substrate and catalytic promiscuity of tryptophan prenyltransferases.,Ostertag E, Zheng L, Broger K, Stehle T, Li SM, Zocher G J Mol Biol. 2020 Nov 26. pii: S0022-2836(20)30644-6. doi:, 10.1016/j.jmb.2020.11.025. PMID:33249189<ref>PMID:33249189</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zry" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 21819]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ostertag E]]	+	[[Category: Ostertag, E]]
-	[[Category: Stehle T]]	+	[[Category: Stehle, T]]
-	[[Category: Zocher G]]	+	[[Category: Zocher, G]]
		+	[[Category: Abba-barrel fold]]
		+	[[Category: Complex]]
		+	[[Category: Prenyltransferase]]
		+	[[Category: Transferase]]

OCA at 06:38, 9 December 2020

OCA — Wed, 09 Dec 2020 06:38:48 GMT

←Older revision		Revision as of 06:38, 9 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6zry is ~~ON HOLD until Paper Publication~~	+	==6-dimethylallyl tryptophan synthase==
-		+	<StructureSection load='6zry' size='340' side='right'caption='[[6zry]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZRY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZRY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zry OCA], [http://pdbe.org/6zry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zry RCSB], [http://www.ebi.ac.uk/pdbsum/6zry PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zry ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ostertag E]]
		+	[[Category: Stehle T]]
		+	[[Category: Zocher G]]

OCA at 09:32, 29 July 2020

OCA — Wed, 29 Jul 2020 09:32:14 GMT

←Older revision		Revision as of 09:32, 29 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6zry is ON HOLD	+	The entry 6zry is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6zry" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 08:18:45 GMT

Protected "6zry" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:18, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zry is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Jul 2020 08:18:43 GMT

New page: '''Unreleased structure''' The entry 6zry is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6zry is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]