OCA at 07:07, 7 April 2021

OCA — Wed, 07 Apr 2021 07:07:00 GMT

←Older revision		Revision as of 07:07, 7 April 2021
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	==Two states of a de novo hexameric coiled coil assembly CC-Type2-(LaIdGe)4==		==Two states of a de novo hexameric coiled coil assembly CC-Type2-(LaIdGe)4==
-	<StructureSection load='6zt1' size='340' side='right'caption='[[6zt1]]' scene=''>	+	<StructureSection load='6zt1' size='340' side='right'caption='[[6zt1]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZT1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zt1]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZT1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zt1 OCA], [https://pdbe.org/6zt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zt1 RCSB], [https://www.ebi.ac.uk/pdbsum/6zt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zt1 ProSAT]</span></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4BF:4-BROMO-L-PHENYLALANINE'>4BF</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zt1 OCA], [https://pdbe.org/6zt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zt1 RCSB], [https://www.ebi.ac.uk/pdbsum/6zt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zt1 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	De novo protein design is advancing rapidly. However, most designs are for single states. Here we report a de novo designed peptide that forms multiple alpha-helical-bundle states that are accessible and interconvertible under the same conditions. Usually in such designs amphipathic alpha helices associate to form compact structures with consolidated hydrophobic cores. However, recent rational and computational designs have delivered open alpha-helical barrels with functionalisable cavities. By placing glycine judiciously in the helical interfaces of an alpha-helical barrel, we obtain both open and compact states in a single protein crystal. Molecular dynamics simulations indicate a free-energy landscape with multiple and interconverting states. Together, these findings suggest a frustrated system in which steric interactions that maintain the open barrel and the hydrophobic effect that drives complete collapse are traded-off. Indeed, addition of a hydrophobic co-solvent that can bind within the barrel affects the switch between the states both in silico and experimentally.
		+
		+	Structural resolution of switchable states of a de novo peptide assembly.,Dawson WM, Lang EJM, Rhys GG, Shelley KL, Williams C, Brady RL, Crump MP, Mulholland AJ, Woolfson DN Nat Commun. 2021 Mar 9;12(1):1530. doi: 10.1038/s41467-021-21851-8. PMID:33750792<ref>PMID:33750792</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zt1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brady RL]]	+	[[Category: Brady, R L]]
-	[[Category: Rhys GG]]	+	[[Category: Rhys, G G]]
-	[[Category: Woolfson DN]]	+	[[Category: Woolfson, D N]]
		+	[[Category: Coiled coil]]
		+	[[Category: De novo peptide]]
		+	[[Category: De novo protein]]
		+	[[Category: Multi-state]]

OCA at 11:09, 3 February 2021

OCA — Wed, 03 Feb 2021 11:09:07 GMT

←Older revision		Revision as of 11:09, 3 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6zt1 is ~~ON HOLD~~	+	==Two states of a de novo hexameric coiled coil assembly CC-Type2-(LaIdGe)4==
-		+	<StructureSection load='6zt1' size='340' side='right'caption='[[6zt1]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZT1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zt1 OCA], [https://pdbe.org/6zt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zt1 RCSB], [https://www.ebi.ac.uk/pdbsum/6zt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zt1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Brady RL]]
		+	[[Category: Rhys GG]]
		+	[[Category: Woolfson DN]]

OCA: Protected "6zt1" [edit=sysop:move=sysop]

OCA — Wed, 29 Jul 2020 09:32:56 GMT

Protected "6zt1" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:32, 29 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zt1 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Jul 2020 09:32:55 GMT

New page: '''Unreleased structure''' The entry 6zt1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6zt1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6zt1 - Revision history

OCA at 07:07, 7 April 2021

OCA at 11:09, 3 February 2021

OCA: Protected "6zt1" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6zt1 is ON HOLD Authors: Description: Category: Unreleased Structures