6zur - Revision history

OCA at 10:54, 15 November 2023

OCA — Wed, 15 Nov 2023 10:54:13 GMT

←Older revision		Revision as of 10:54, 15 November 2023
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	==Psychrophilic aromatic amino acids aminotransferase from Psychrobacter sp. B6 cocrystalized with substrate analog - L-p-hydroxyphenyllactic acid==		==Psychrophilic aromatic amino acids aminotransferase from Psychrobacter sp. B6 cocrystalized with substrate analog - L-p-hydroxyphenyllactic acid==
-	<StructureSection load='6zur' size='340' side='right'caption='[[6zur]]' scene=''>	+	<StructureSection load='6zur' size='340' side='right'caption='[[6zur]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zur]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Psychrobacter_sp._B6 Psychrobacter sp. B6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZUR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zur OCA], [https://pdbe.org/6zur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zur RCSB], [https://www.ebi.ac.uk/pdbsum/6zur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zur ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.31Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=TYF:(2S)-2-HYDROXY-3-(4-HYDROXYPHENYL)PROPANOIC+ACID'>TYF</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zur OCA], [https://pdbe.org/6zur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zur RCSB], [https://www.ebi.ac.uk/pdbsum/6zur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zur ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C7E5X4_9GAMM C7E5X4_9GAMM]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aromatic amino acid aminotransferases present a special potential in the production of drugs and synthons, thanks to their ability to accommodate a wider range of substrates in their active site, in contrast to aliphatic amino acid aminotransferases. The mechanism of active site adjustment toward substrates of psychrophilic aromatic amino acid aminotransferase (PsyArAT) from Psychrobacter sp. B6 is discussed based on crystal structures of complexes with four hydroxy-analogs of substrates: phenylalanine, tyrosine, tryptophan and aspartic acid. These competitive inhibitors are bound in the active center of PsyArAT but do not undergo transamination reaction, which makes them an outstanding tool for examination of the enzyme catalytic center. The use of hydroxy-acids enabled insight into substrate binding by native PsyArAT, without mutating the catalytic lysine and modifying cofactor interactions. Thus, the binding mode of substrates and the resulting analysis of the volume of the catalytic site is close to a native condition. Observation of these inhibitors' binding allows for explanation of the enzyme's adaptability to process various sizes of substrates and to gain knowledge about its potential biotechnological application. Depending on the character and size of the used inhibitors, the enzyme crystallized in different space groups and showed conformational changes of the active site upon ligand binding.
		+
		+	Structural Evidence of Active Site Adaptability towards Different Sized Substrates of Aromatic Amino Acid Aminotransferase from Psychrobacter Sp. B6.,Bujacz A, Rum J, Rutkiewicz M, Pietrzyk-Brzezinska AJ, Bujacz G Materials (Basel). 2021 Jun 17;14(12). pii: ma14123351. doi: 10.3390/ma14123351. PMID:34204354<ref>PMID:34204354</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zur" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Psychrobacter sp. B6]]
	[[Category: Bujacz A]]		[[Category: Bujacz A]]
	[[Category: Bujacz G]]		[[Category: Bujacz G]]

OCA at 09:50, 14 July 2021

OCA — Wed, 14 Jul 2021 09:50:51 GMT

←Older revision		Revision as of 09:50, 14 July 2021
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-	'''Unreleased structure'''

-	~~The entry 6zur is ON HOLD until Paper Publication~~	+	==Psychrophilic aromatic amino acids aminotransferase from Psychrobacter sp. B6 cocrystalized with substrate analog - L-p-hydroxyphenyllactic acid==
-		+	<StructureSection load='6zur' size='340' side='right'caption='[[6zur]]' scene=''>
-	~~Authors: Bujacz, A., Rum, J., Rutkiewicz, M., Pietrzyk-Brzezinska, A.J., Bujacz, G.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZUR FirstGlance]. <br>
-	~~Description:~~ Psychrophilic aromatic amino acids aminotransferase from Psychrobacter sp. B6 cocrystalized with substrate analog -L-p-hydroxyphenyllactic acid	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zur OCA], [https://pdbe.org/6zur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zur RCSB], [https://www.ebi.ac.uk/pdbsum/6zur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zur ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Rutkiewicz~~, M]]	+	__TOC__
-	[[~~Category~~: ~~Pietrzyk~~-~~Brzezinska, A~~.J]]	+	</StructureSection>
-	[[Category: Bujacz, A]]	+	[[Category: Large Structures]]
-	[[Category: Bujacz, G]]	+	[[Category: Bujacz A]]
-	[[Category: Rum, J]]	+	[[Category: Bujacz G]]
		+	[[Category: Pietrzyk-Brzezinska AJ]]
		+	[[Category: Rum J]]
		+	[[Category: Rutkiewicz M]]

OCA at 04:56, 2 September 2020

OCA — Wed, 02 Sep 2020 04:56:50 GMT

←Older revision		Revision as of 04:56, 2 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6zur is ON HOLD	+	The entry 6zur is ON HOLD until Paper Publication

	Authors: Bujacz, A., Rum, J., Rutkiewicz, M., Pietrzyk-Brzezinska, A.J., Bujacz, G.		Authors: Bujacz, A., Rum, J., Rutkiewicz, M., Pietrzyk-Brzezinska, A.J., Bujacz, G.
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	[[Category: Rutkiewicz, M]]		[[Category: Rutkiewicz, M]]
	[[Category: Pietrzyk-Brzezinska, A.J]]		[[Category: Pietrzyk-Brzezinska, A.J]]
-	[[Category: Bujacz, G]]
	[[Category: Bujacz, A]]		[[Category: Bujacz, A]]
		+	[[Category: Bujacz, G]]
	[[Category: Rum, J]]		[[Category: Rum, J]]

OCA: Protected "6zur" [edit=sysop:move=sysop]

OCA — Wed, 29 Jul 2020 09:37:55 GMT

Protected "6zur" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:37, 29 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zur is ON HOLD Authors: Bujacz, A., Rum, J., Rutkiewicz, M., Pietrzyk-Brzezinska, A.J., Bujacz, G. Description: Psychrophilic aromatic amino acids...

OCA — Wed, 29 Jul 2020 09:37:53 GMT

New page: '''Unreleased structure''' The entry 6zur is ON HOLD Authors: Bujacz, A., Rum, J., Rutkiewicz, M., Pietrzyk-Brzezinska, A.J., Bujacz, G. Description: Psychrophilic aromatic amino acids...

New page

'''Unreleased structure'''

The entry 6zur is ON HOLD

Authors: Bujacz, A., Rum, J., Rutkiewicz, M., Pietrzyk-Brzezinska, A.J., Bujacz, G.

Description: Psychrophilic aromatic amino acids aminotransferase from Psychrobacter sp. B6 cocrystalized with substrate analog -L-p-hydroxyphenyllactic acid
[[Category: Unreleased Structures]]
[[Category: Rutkiewicz, M]]
[[Category: Pietrzyk-Brzezinska, A.J]]
[[Category: Bujacz, G]]
[[Category: Bujacz, A]]
[[Category: Rum, J]]