7a0o - Revision history

OCA at 11:09, 14 June 2023

OCA — Wed, 14 Jun 2023 11:09:51 GMT

←Older revision		Revision as of 11:09, 14 June 2023
Line 7:		Line 7:
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A2S2B4P9_TRICX A0A2S2B4P9_TRICX]]	+	[https://www.uniprot.org/uniprot/A0A2S2B4P9_TRICX A0A2S2B4P9_TRICX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:42, 7 September 2022

OCA — Wed, 07 Sep 2022 19:42:51 GMT

←Older revision		Revision as of 19:42, 7 September 2022
Line 1:		Line 1:

	==NMR structure of flagelliform spidroin (FlagSp) N-terminal domain from Trichonephila clavipes at pH 5.5==		==NMR structure of flagelliform spidroin (FlagSp) N-terminal domain from Trichonephila clavipes at pH 5.5==
-	<StructureSection load='7a0o' size='340' side='right'caption='[[7a0o~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='7a0o' size='340' side='right'caption='[[7a0o]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7a0o]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a0o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichonephila_clavipes Trichonephila clavipes]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0O FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0o OCA], [https://pdbe.org/7a0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0o RCSB], [https://www.ebi.ac.uk/pdbsum/7a0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0o ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0o OCA], [https://pdbe.org/7a0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0o RCSB], [https://www.ebi.ac.uk/pdbsum/7a0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A2S2B4P9_TRICX A0A2S2B4P9_TRICX]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jaudzems, K]]	+	[[Category: Trichonephila clavipes]]
-	[[Category: Johansson, J]]	+	[[Category: Jaudzems K]]
-	[[Category: Kaldmae, M]]	+	[[Category: Johansson J]]
-	[[Category: Kitoka, K]]	+	[[Category: Kaldmae M]]
-	[[Category: Kronqvist, N]]	+	[[Category: Kitoka K]]
-	[[Category: Landreh, M]]	+	[[Category: Kronqvist N]]
-	[[Category: Rising, A]]	+	[[Category: Landreh M]]
-	[[Category: Sarr, M]]	+	[[Category: Rising A]]
-	[[Category: Walsh-White, K A]]	+	[[Category: Sarr M]]
-	~~[[Category: Flagelliform spidroin]]~~	+	[[Category: Walsh-White K-A]]
-	~~[[Category: Nt domain]]~~	+
-	~~[[Category: Ph 5 5]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 06:41, 31 August 2022

OCA — Wed, 31 Aug 2022 06:41:33 GMT

←Older revision		Revision as of 06:41, 31 August 2022
Line 1:		Line 1:

	==NMR structure of flagelliform spidroin (FlagSp) N-terminal domain from Trichonephila clavipes at pH 5.5==		==NMR structure of flagelliform spidroin (FlagSp) N-terminal domain from Trichonephila clavipes at pH 5.5==
-	<StructureSection load='7a0o' size='340' side='right'caption='[[7a0o]]' scene=''>	+	<StructureSection load='7a0o' size='340' side='right'caption='[[7a0o]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a0o]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0O FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0o OCA], [https://pdbe.org/7a0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0o RCSB], [https://www.ebi.ac.uk/pdbsum/7a0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0o ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0o OCA], [https://pdbe.org/7a0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0o RCSB], [https://www.ebi.ac.uk/pdbsum/7a0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0o ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The N-terminal (NT) domain of spider silk proteins (spidroins) is crucial for their storage at high concentrations and also regulates silk assembly. NTs from the major ampullate spidroin (MaSp) and the minor ampullate spidroin are monomeric at neutral pH and confer solubility to spidroins, whereas at lower pH, they dimerize to interconnect spidroins in a fiber. This dimerization is known to result from modulation of electrostatic interactions by protonation of well-conserved glutamates, although it is undetermined if this mechanism applies to other spidroin types as well. Here, we determine the solution and crystal structures of the flagelliform spidroin NT, which shares only 35% identity with MaSp NT, and investigate the mechanisms of its dimerization. We show that flagelliform spidroin NT is structurally similar to MaSp NT and that the electrostatic intermolecular interaction between Asp 40 and Lys 65 residues is conserved. However, the protonation events involve a different set of residues than in MaSp, indicating that an overall mechanism of pH-dependent dimerization is conserved but can be mediated by different pathways in different silk types.
		+
		+	The dimerization mechanism of the N-terminal domain of spider silk proteins is conserved despite extensive sequence divergence.,Sarr M, Kitoka K, Walsh-White KA, Kaldmae M, Metlans R, Tars K, Mantese A, Shah D, Landreh M, Rising A, Johansson J, Jaudzems K, Kronqvist N J Biol Chem. 2022 May;298(5):101913. doi: 10.1016/j.jbc.2022.101913. Epub 2022, Apr 7. PMID:35398358<ref>PMID:35398358</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a0o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jaudzems K]]	+	[[Category: Jaudzems, K]]
-	[[Category: Johansson J]]	+	[[Category: Johansson, J]]
-	[[Category: Kaldmae M]]	+	[[Category: Kaldmae, M]]
-	[[Category: Kitoka K]]	+	[[Category: Kitoka, K]]
-	[[Category: Kronqvist N]]	+	[[Category: Kronqvist, N]]
-	[[Category: Landreh M]]	+	[[Category: Landreh, M]]
-	[[Category: Rising A]]	+	[[Category: Rising, A]]
-	[[Category: Sarr M]]	+	[[Category: Sarr, M]]
-	[[Category: Walsh-White K-A]]	+	[[Category: Walsh-White, K A]]
		+	[[Category: Flagelliform spidroin]]
		+	[[Category: Nt domain]]
		+	[[Category: Ph 5 5]]
		+	[[Category: Structural protein]]

OCA at 06:06, 18 August 2021

OCA — Wed, 18 Aug 2021 06:06:38 GMT

←Older revision		Revision as of 06:06, 18 August 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7a0o is ON HOLD until Paper Publication~~	+	==NMR structure of flagelliform spidroin (FlagSp) N-terminal domain from Trichonephila clavipes at pH 5.5==
-		+	<StructureSection load='7a0o' size='340' side='right'caption='[[7a0o]]' scene=''>
-	~~Authors: Sarr, M., Kitoka, K., Walsh-White, K.-A., Kaldmae, M., Landreh, M., Rising, A., Johansson, J., Jaudzems, K., Kronqvist, N.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0O FirstGlance]. <br>
-	~~Description:~~ NMR structure of flagelliform spidroin (FlagSp) N-terminal domain from Trichonephila clavipes at pH 5.5	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0o OCA], [https://pdbe.org/7a0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0o RCSB], [https://www.ebi.ac.uk/pdbsum/7a0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0o ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Johansson~~, J]]	+	__TOC__
-	[[Category: ~~Landreh, M~~]]	+	</StructureSection>
-	[[Category: Jaudzems, K]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sarr, M~~]]	+	[[Category: Jaudzems K]]
-	[[Category: Kaldmae, M]]	+	[[Category: Johansson J]]
-	[[Category: ~~Rising, A~~]]	+	[[Category: Kaldmae M]]
-	[[Category: Kronqvist, N]]	+	[[Category: Kitoka K]]
-	[[Category: ~~Kitoka, K~~]]	+	[[Category: Kronqvist N]]
-	[[Category: Walsh-White, K.-A]]	+	[[Category: Landreh M]]
		+	[[Category: Rising A]]
		+	[[Category: Sarr M]]
		+	[[Category: Walsh-White K-A]]

OCA at 07:11, 9 September 2020

OCA — Wed, 09 Sep 2020 07:11:31 GMT

←Older revision		Revision as of 07:11, 9 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a0o is ON HOLD	+	The entry 7a0o is ON HOLD until Paper Publication

	Authors: Sarr, M., Kitoka, K., Walsh-White, K.-A., Kaldmae, M., Landreh, M., Rising, A., Johansson, J., Jaudzems, K., Kronqvist, N.		Authors: Sarr, M., Kitoka, K., Walsh-White, K.-A., Kaldmae, M., Landreh, M., Rising, A., Johansson, J., Jaudzems, K., Kronqvist, N.

OCA: Protected "7a0o" [edit=sysop:move=sysop]

OCA — Wed, 19 Aug 2020 04:37:18 GMT

Protected "7a0o" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:37, 19 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a0o is ON HOLD Authors: Sarr, M., Kitoka, K., Walsh-White, K.-A., Kaldmae, M., Landreh, M., Rising, A., Johansson, J., Jaudzems, K., Kronqvist, N. ...

OCA — Wed, 19 Aug 2020 04:37:17 GMT

New page: '''Unreleased structure''' The entry 7a0o is ON HOLD Authors: Sarr, M., Kitoka, K., Walsh-White, K.-A., Kaldmae, M., Landreh, M., Rising, A., Johansson, J., Jaudzems, K., Kronqvist, N. ...

New page

'''Unreleased structure'''

The entry 7a0o is ON HOLD

Authors: Sarr, M., Kitoka, K., Walsh-White, K.-A., Kaldmae, M., Landreh, M., Rising, A., Johansson, J., Jaudzems, K., Kronqvist, N.

Description: NMR structure of flagelliform spidroin (FlagSp) N-terminal domain from Trichonephila clavipes at pH 5.5
[[Category: Unreleased Structures]]
[[Category: Johansson, J]]
[[Category: Landreh, M]]
[[Category: Jaudzems, K]]
[[Category: Sarr, M]]
[[Category: Kaldmae, M]]
[[Category: Rising, A]]
[[Category: Kronqvist, N]]
[[Category: Kitoka, K]]
[[Category: Walsh-White, K.-A]]