7a2g - Revision history

OCA at 09:51, 22 June 2022

OCA — Wed, 22 Jun 2022 09:51:18 GMT

←Older revision		Revision as of 09:51, 22 June 2022
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	==Full-length structure of the substrate-free tyrosine hydroxylase (apo-TH).==		==Full-length structure of the substrate-free tyrosine hydroxylase (apo-TH).==
-	<StructureSection load='7a2g' size='340' side='right'caption='[[7a2g]]' scene=''>	+	<StructureSection load='7a2g' size='340' side='right'caption='[[7a2g]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a2g]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A2G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a2g OCA], [https://pdbe.org/7a2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a2g RCSB], [https://www.ebi.ac.uk/pdbsum/7a2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a2g ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tyrosine_3-monooxygenase Tyrosine 3-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.2 1.14.16.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a2g OCA], [https://pdbe.org/7a2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a2g RCSB], [https://www.ebi.ac.uk/pdbsum/7a2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a2g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/TY3H_HUMAN TY3H_HUMAN]] Autosomal recessive dopa-responsive dystonia. The disease is caused by mutations affecting the gene represented in this entry. May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.<ref>PMID:20809526</ref>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/TY3H_HUMAN TY3H_HUMAN]] Plays an important role in the physiology of adrenergic neurons.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthesis of dopamine (DA) and other catecholamines, and its dysfunction leads to DA deficiency and parkinsonisms. Inhibition by catecholamines and reactivation by S40 phosphorylation are key regulatory mechanisms of TH activity and conformational stability. We used Cryo-EM to determine the structures of full-length human TH without and with DA, and the structure of S40 phosphorylated TH, complemented with biophysical and biochemical characterizations and molecular dynamics simulations. TH presents a tetrameric structure with dimerized regulatory domains that are separated 15 A from the catalytic domains. Upon DA binding, a 20-residue alpha-helix in the flexible N-terminal tail of the regulatory domain is fixed in the active site, blocking it, while S40-phosphorylation forces its egress. The structures reveal the molecular basis of the inhibitory and stabilizing effects of DA and its counteraction by S40-phosphorylation, key regulatory mechanisms for homeostasis of DA and TH.
		+
		+	Structural mechanism for tyrosine hydroxylase inhibition by dopamine and reactivation by Ser40 phosphorylation.,Bueno-Carrasco MT, Cuellar J, Flydal MI, Santiago C, Krakenes TA, Kleppe R, Lopez-Blanco JR, Marcilla M, Teigen K, Alvira S, Chacon P, Martinez A, Valpuesta JM Nat Commun. 2022 Jan 10;13(1):74. doi: 10.1038/s41467-021-27657-y. PMID:35013193<ref>PMID:35013193</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a2g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bueno-Carrasco MT]]	+	[[Category: Tyrosine 3-monooxygenase]]
-	[[Category: Cuellar J]]	+	[[Category: Bueno-Carrasco, M T]]
-	[[Category: Flydal MI]]	+	[[Category: Cuellar, J]]
-	[[Category: Martinez A]]	+	[[Category: Flydal, M I]]
-	[[Category: Santiago C]]	+	[[Category: Martinez, A]]
-	[[Category: Valpuesta JM]]	+	[[Category: Santiago, C]]
		+	[[Category: Valpuesta, J M]]
		+	[[Category: Brain]]
		+	[[Category: Catecholamine]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Parkinson]]
		+	[[Category: Tetramer]]

OCA at 06:17, 1 December 2021

OCA — Wed, 01 Dec 2021 06:17:19 GMT

←Older revision		Revision as of 06:17, 1 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7a2g is ~~ON HOLD until Paper Publication~~	+	==Full-length structure of the substrate-free tyrosine hydroxylase (apo-TH).==
-		+	<StructureSection load='7a2g' size='340' side='right'caption='[[7a2g]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A2G FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a2g OCA], [https://pdbe.org/7a2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a2g RCSB], [https://www.ebi.ac.uk/pdbsum/7a2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a2g ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bueno-Carrasco MT]]
		+	[[Category: Cuellar J]]
		+	[[Category: Flydal MI]]
		+	[[Category: Martinez A]]
		+	[[Category: Santiago C]]
		+	[[Category: Valpuesta JM]]

OCA at 03:58, 14 October 2020

OCA — Wed, 14 Oct 2020 03:58:03 GMT

←Older revision		Revision as of 03:58, 14 October 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a2g is ON HOLD	+	The entry 7a2g is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 06:37, 16 September 2020

OCA — Wed, 16 Sep 2020 06:37:23 GMT

←Older revision		Revision as of 06:37, 16 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a2g is ON HOLD ~~until sometime in the future~~	+	The entry 7a2g is ON HOLD

	Authors:		Authors:

OCA: Protected "7a2g" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2020 09:13:20 GMT

Protected "7a2g" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:13, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a2g is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Aug 2020 09:13:18 GMT

New page: '''Unreleased structure''' The entry 7a2g is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7a2g is ON HOLD until sometime in the future

Authors:

Description:
[[Category: Unreleased Structures]]