OCA at 12:03, 1 February 2024

OCA — Thu, 01 Feb 2024 12:03:14 GMT

←Older revision		Revision as of 12:03, 1 February 2024
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-	====	+	==Crystal structure of dengue 1 virus envelope glycoprotein in complex with the scFv fragment of the broadly neutralizing human antibody EDE1 C10==
-	<StructureSection load='7a3o' size='340' side='right'caption='[[7a3o]]' scene=''>	+	<StructureSection load='7a3o' size='340' side='right'caption='[[7a3o]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a3o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_1 Dengue virus 1] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A3O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3o OCA], [https://pdbe.org/7a3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3o RCSB], [https://www.ebi.ac.uk/pdbsum/7a3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3o ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3o OCA], [https://pdbe.org/7a3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3o RCSB], [https://www.ebi.ac.uk/pdbsum/7a3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9II02_9FLAV Q9II02_9FLAV] Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host immune response.[ARBA:ARBA00024317] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504] Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3.[PROSITE-ProRule:PRU00859]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The human monoclonal antibody C10 exhibits extraordinary cross-reactivity, potently neutralizing Zika virus (ZIKV) and the four serotypes of dengue virus (DENV1-DENV4). Here we describe a comparative structure-function analysis of C10 bound to the envelope (E) protein dimers of the five viruses it neutralizes. We demonstrate that the C10 Fab has high affinity for ZIKV and DENV1 but not for DENV2, DENV3, and DENV4. We further show that the C10 interaction with the latter viruses requires an E protein conformational landscape that limits binding to only one of the three independent epitopes per virion. This limited affinity is nevertheless counterbalanced by the particle's icosahedral organization, which allows two different dimers to be reached by both Fab arms of a C10 immunoglobulin. The epitopes' geometric distribution thus confers C10 its exceptional neutralization breadth. Our results highlight the importance not only of paratope/epitope complementarity but also the topological distribution for epitope-focused vaccine design.
		+
		+	The epitope arrangement on flavivirus particles contributes to Mab C10's extraordinary neutralization breadth across Zika and dengue viruses.,Sharma A, Zhang X, Dejnirattisai W, Dai X, Gong D, Wongwiwat W, Duquerroy S, Rouvinski A, Vaney MC, Guardado-Calvo P, Haouz A, England P, Sun R, Zhou ZH, Mongkolsapaya J, Screaton GR, Rey FA Cell. 2021 Dec 9;184(25):6052-6066.e18. doi: 10.1016/j.cell.2021.11.010. Epub, 2021 Nov 30. PMID:34852239<ref>PMID:34852239</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a3o" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dengue virus 1]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Duquerroy S]]
		+	[[Category: Guardado-Calvo P]]
		+	[[Category: Rey FA]]
		+	[[Category: Rouvinski A]]
		+	[[Category: Sharma A]]
		+	[[Category: Vaney MC]]

OCA at 09:52, 8 December 2021

OCA — Wed, 08 Dec 2021 09:52:51 GMT

←Older revision		Revision as of 09:52, 8 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7a3o ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7a3o' size='340' side='right'caption='[[7a3o]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3o OCA], [https://pdbe.org/7a3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3o RCSB], [https://www.ebi.ac.uk/pdbsum/7a3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3o ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:02, 4 August 2021

OCA — Wed, 04 Aug 2021 09:02:33 GMT

←Older revision		Revision as of 09:02, 4 August 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a3o is ON HOLD	+	The entry 7a3o is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7a3o" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2020 09:16:09 GMT

Protected "7a3o" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:16, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a3o is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Aug 2020 09:16:08 GMT

New page: '''Unreleased structure''' The entry 7a3o is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7a3o is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7a3o - Revision history

OCA at 12:03, 1 February 2024

OCA at 09:52, 8 December 2021

OCA at 09:02, 4 August 2021

OCA: Protected "7a3o" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7a3o is ON HOLD Authors: Description: Category: Unreleased Structures