OCA at 12:03, 1 February 2024

OCA — Thu, 01 Feb 2024 12:03:45 GMT

←Older revision		Revision as of 12:03, 1 February 2024
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	==Crystal structure of lid-truncated apo BiP in an oligomeric state==		==Crystal structure of lid-truncated apo BiP in an oligomeric state==
-	<StructureSection load='7a4u' size='340' side='right'caption='[[7a4u]]' scene=''>	+	<StructureSection load='7a4u' size='340' side='right'caption='[[7a4u]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A4U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7A4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a4u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A4U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7a4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a4u OCA], [~~http~~://pdbe.org/7a4u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7a4u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7a4u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7a4u ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a4u OCA], [https://pdbe.org/7a4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a4u RCSB], [https://www.ebi.ac.uk/pdbsum/7a4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a4u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BIP_CRIGR BIP_CRIGR] Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (By similarity). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:29198525). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (PubMed:29198525). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (PubMed:29198525). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.[UniProtKB:P11021][UniProtKB:P20029]<ref>PMID:29198525</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The metazoan endoplasmic reticulum (ER) serves both as a hub for maturation of secreted proteins and as an intracellular calcium storage compartment, facilitating calcium release-dependent cellular processes. ER calcium depletion robustly activates the unfolded protein response (UPR). However, it is unclear how fluctuations in ER calcium impact organellar proteostasis. Here we report that calcium selectively affects the dynamics of the abundant metazoan ER Hsp70 chaperone BiP, by enhancing its affinity for ADP. In the calcium-replete ER, ADP rebinding to post-ATP hydrolysis BiP-substrate complexes competes with ATP binding during both spontaneous and co-chaperone-assisted nucleotide exchange, favouring substrate retention. Conversely, in the calcium-depleted ER, relative acceleration of ADP-to-ATP exchange favours substrate release. These findings explain the rapid dissociation of certain substrates from BiP observed in the calcium-depleted ER and suggest a mechanism for tuning ER quality control and coupling UPR activity to signals that mobilise ER calcium in secretory cells.
		+
		+	Calcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes.,Preissler S, Rato C, Yan Y, Perera LA, Czako A, Ron D Elife. 2020 Dec 9;9. pii: 62601. doi: 10.7554/eLife.62601. PMID:33295873<ref>PMID:33295873</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a4u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cricetulus griseus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Perera LA]]		[[Category: Perera LA]]
	[[Category: Ron D]]		[[Category: Ron D]]

OCA at 12:33, 16 December 2020

OCA — Wed, 16 Dec 2020 12:33:33 GMT

←Older revision		Revision as of 12:33, 16 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7a4u ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of lid-truncated apo BiP in an oligomeric state==
-		+	<StructureSection load='7a4u' size='340' side='right'caption='[[7a4u]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A4U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7A4U FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7a4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a4u OCA], [http://pdbe.org/7a4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7a4u RCSB], [http://www.ebi.ac.uk/pdbsum/7a4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7a4u ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Perera LA]]
		+	[[Category: Ron D]]

OCA at 05:16, 4 November 2020

OCA — Wed, 04 Nov 2020 05:16:37 GMT

←Older revision		Revision as of 05:16, 4 November 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a4u is ON HOLD	+	The entry 7a4u is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7a4u" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2020 09:18:21 GMT

Protected "7a4u" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:18, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a4u is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Aug 2020 09:18:20 GMT

New page: '''Unreleased structure''' The entry 7a4u is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7a4u is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7a4u - Revision history

OCA at 12:03, 1 February 2024

OCA at 12:33, 16 December 2020

OCA at 05:16, 4 November 2020

OCA: Protected "7a4u" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7a4u is ON HOLD Authors: Description: Category: Unreleased Structures