7a54 - Revision history

OCA at 12:03, 1 February 2024

OCA — Thu, 01 Feb 2024 12:03:53 GMT

←Older revision		Revision as of 12:03, 1 February 2024
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	<StructureSection load='7a54' size='340' side='right'caption='[[7a54]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='7a54' size='340' side='right'caption='[[7a54]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7a54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus~~ pneumoniae~~" (klein 1884) weichselbaum 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A54 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A54 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DAN:2-DEOXY~~-2~~,3-DEHYDRO-N-ACETYL-NEURAMINIC+ACID'>DAN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>nanA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1313~~ "~~Diplococcus pneumoniae~~" ~~(Klein 1884) Weichselbaum 1886])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAN:2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC+ACID'>DAN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Exo~~-~~alpha~~-~~sialidase Exo~~-~~alpha~~-~~sialidase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a54 OCA], [https://pdbe.org/7a54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a54 RCSB], [https://www.ebi.ac.uk/pdbsum/7a54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a54 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a54 OCA], [https://pdbe.org/7a54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a54 RCSB], [https://www.ebi.ac.uk/pdbsum/7a54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a54 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NANA_STREE NANA_STREE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Exo-alpha-sialidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bouckaert, J~~]]	+	[[Category: Streptococcus pneumoniae]]
-	[[Category: ~~Bridot, C~~]]	+	[[Category: Bouckaert J]]
-	[[Category: ~~Catalytic domain]]~~	+	[[Category: Bridot C]]
-	~~[[Category: Dana]]~~	+
-	~~[[Category: Sialidase]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 15:00, 3 March 2021

OCA — Wed, 03 Mar 2021 15:00:26 GMT

←Older revision		Revision as of 15:00, 3 March 2021
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	==Two copies of the catalytic domain of NanA sialidase from Streptococcus pneumoniae juxtaposed in the P212121 space group, in complex with DANA==		==Two copies of the catalytic domain of NanA sialidase from Streptococcus pneumoniae juxtaposed in the P212121 space group, in complex with DANA==
-	<StructureSection load='7a54' size='340' side='right'caption='[[7a54]]' scene=''>	+	<StructureSection load='7a54' size='340' side='right'caption='[[7a54]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A54 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7A54 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A54 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7a54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a54 OCA], [~~http~~://pdbe.org/7a54 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7a54 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7a54 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7a54 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAN:2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC+ACID'>DAN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a54 OCA], [https://pdbe.org/7a54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a54 RCSB], [https://www.ebi.ac.uk/pdbsum/7a54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a54 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial sialidases (SA) are validated drug targets expressed by common human pathogens such as Streptococcus pneumoniae, Vibrio cholerae or Clostridium perfringens. Non-covalent inhibitors of bacterial SA capable of reaching the submicromolar level are rarely reported. We developed multi- and polyvalent compounds based on the transition state analogue 2-deoxy-2,3-didehydro-N-acetylneuraminic (DANA). Poly-DANA inhibits the catalytic activity of SA from S. pneumoniae (NanA) and the symbiotic microorganism B. thetaiotaomicron (BtSA) at the picomolar and low nanomolar levels when expressed in moles of molecules and of DANA, respectively. Each DANA grafted to the polymer surpasses the inhibitory potential of the monovalent analogue by more than four orders of magnitude, which represents the highest multivalent effect reported so far for an enzyme inhibition. The synergistic interaction was shown to operate in the catalytic domain exclusively, and not in the flanked carbohydrate-binding module (CBM). These results offers interesting perspectives for the multivalent inhibition of other SA families lacking a CBM, such as viral, parasitic or human SA..
		+
		+	Polyvalent transition-state analogues of sialyl substrates strongly inhibit bacterial sialidases.,Assailly C, Bridot C, Saumonneau A, Lottin P, Roubinet B, Krammer EM, Francois F, Vena F, Landemarre L, Alvarez-Dorta D, Deniaud D, Grandjean C, Tellier C, Pascual S, Montembault V, Fontaine L, Daligault F, Bouckaert J, Gouin SG Chemistry. 2020 Nov 5. doi: 10.1002/chem.202004672. PMID:33150981<ref>PMID:33150981</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a54" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Exo-alpha-sialidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bouckaert J]]	+	[[Category: Bouckaert, J]]
-	[[Category: Bridot C]]	+	[[Category: Bridot, C]]
		+	[[Category: Catalytic domain]]
		+	[[Category: Dana]]
		+	[[Category: Sialidase]]
		+	[[Category: Structural protein]]

OCA at 09:39, 18 November 2020

OCA — Wed, 18 Nov 2020 09:39:49 GMT

←Older revision		Revision as of 09:39, 18 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7a54 ~~is ON HOLD until Paper Publication~~	+	==Two copies of the catalytic domain of NanA sialidase from Streptococcus pneumoniae juxtaposed in the P212121 space group, in complex with DANA==
-		+	<StructureSection load='7a54' size='340' side='right'caption='[[7a54]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A54 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7A54 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7a54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a54 OCA], [http://pdbe.org/7a54 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7a54 RCSB], [http://www.ebi.ac.uk/pdbsum/7a54 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7a54 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bouckaert J]]
		+	[[Category: Bridot C]]

OCA at 08:37, 23 September 2020

OCA — Wed, 23 Sep 2020 08:37:20 GMT

←Older revision		Revision as of 08:37, 23 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a54 is ON HOLD	+	The entry 7a54 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7a54" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2020 09:19:01 GMT

Protected "7a54" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:19, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a54 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Aug 2020 09:18:59 GMT

New page: '''Unreleased structure''' The entry 7a54 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7a54 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]