7a74 - Revision history

OCA at 12:04, 1 February 2024

OCA — Thu, 01 Feb 2024 12:04:44 GMT

←Older revision		Revision as of 12:04, 1 February 2024
Line 3:		Line 3:
	<StructureSection load='7a74' size='340' side='right'caption='[[7a74]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='7a74' size='340' side='right'caption='[[7a74]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7a74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A74 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A74 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class~~=~~"sblockDat">blaC, blaA, Rv2068c, MTCY49.07c ([https~~:~~//www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a74 OCA], [https://pdbe.org/7a74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a74 RCSB], [https://www.ebi.ac.uk/pdbsum/7a74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a74 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a74 OCA], [https://pdbe.org/7a74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a74 RCSB], [https://www.ebi.ac.uk/pdbsum/7a74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a74 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BLAC_MYCTU BLAC_MYCTU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Ahmad~~, M U~~]]	+	[[Category: Ahmad MU]]
-	[[Category: Chikunova, A]]	+	[[Category: Chikunova A]]
-	[[Category: Perrakis, A]]	+	[[Category: Perrakis A]]
-	[[Category: Ubbink, M]]	+	[[Category: Ubbink M]]
-	~~[[Category: Blac]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mycobacterium tuberculosis~~]]	+

OCA at 10:45, 28 July 2021

OCA — Wed, 28 Jul 2021 10:45:05 GMT

←Older revision		Revision as of 10:45, 28 July 2021
Line 3:		Line 3:
	<StructureSection load='7a74' size='340' side='right'caption='[[7a74]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='7a74' size='340' side='right'caption='[[7a74]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7a74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A74 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A74 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaC, blaA, Rv2068c, MTCY49.07c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaC, blaA, Rv2068c, MTCY49.07c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a74 OCA], [https://pdbe.org/7a74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a74 RCSB], [https://www.ebi.ac.uk/pdbsum/7a74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a74 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a74 OCA], [https://pdbe.org/7a74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a74 RCSB], [https://www.ebi.ac.uk/pdbsum/7a74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a74 ProSAT]</span></td></tr>
Line 25:		Line 25:
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Ahmad, M U]]		[[Category: Ahmad, M U]]
	[[Category: Chikunova, A]]		[[Category: Chikunova, A]]

OCA at 12:27, 9 June 2021

OCA — Wed, 09 Jun 2021 12:27:02 GMT

←Older revision		Revision as of 12:27, 9 June 2021
Line 1:		Line 1:

	==Structure of G132N BlaC from Mycobacterium tuberculosis==		==Structure of G132N BlaC from Mycobacterium tuberculosis==
-	<StructureSection load='7a74' size='340' side='right'caption='[[7a74]]' scene=''>	+	<StructureSection load='7a74' size='340' side='right'caption='[[7a74]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A74 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A74 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a74 OCA], [https://pdbe.org/7a74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a74 RCSB], [https://www.ebi.ac.uk/pdbsum/7a74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a74 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaC, blaA, Rv2068c, MTCY49.07c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a74 OCA], [https://pdbe.org/7a74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a74 RCSB], [https://www.ebi.ac.uk/pdbsum/7a74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a74 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The beta-lactamase of Mycobacterium tuberculosis, BlaC, is susceptible to inhibition by clavulanic acid. The ability of this enzyme to escape inhibition through mutation was probed using error-prone PCR combined with functional screening in Escherichia coli The variant that was found to confer most inhibitor resistance, K234R, as well as variant G132N that was found previously, were characterized using X-ray crystallography and NMR relaxation experiments to probe structural and dynamic properties. The G132N mutant exists in solution in two, almost equally populated conformations that exchange with a rate of ca 88 s(-1) The conformational change affects a broad region of the enzyme. The crystal structure reveals that the Asn132 side chain forces the peptide bond between Ser104 and Ile105 in a cis-conformation. The crystal structure suggests multiple conformations for several side chains (e.g. Ser104, Ser130) and a short loop (214-216). In the K234R mutant, the active site dynamics are significantly diminished with respect to the wild type enzyme. These results show that multiple evolutionary routes are available to increase inhibitor resistance in BlaC and that active site dynamics on the millisecond time scale are not required for catalytic function.
		+
		+	Two beta-lactamase variants with reduced clavulanic acid inhibition display different millisecond dynamics.,Elings W, Chikunova A, van Zanten DB, Drenth R, Ahmad MUD, Blok AJ, Timmer M, Perrakis A, Ubbink M Antimicrob Agents Chemother. 2021 May 24. pii: AAC.02628-20. doi:, 10.1128/AAC.02628-20. PMID:34031049<ref>PMID:34031049</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a74" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ahmad MU]]	+	[[Category: Ahmad, M U]]
-	[[Category: Chikunova A]]	+	[[Category: Chikunova, A]]
-	[[Category: Perrakis A]]	+	[[Category: Perrakis, A]]
-	[[Category: Ubbink M]]	+	[[Category: Ubbink, M]]
		+	[[Category: Blac]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mycobacterium tuberculosis]]

OCA at 10:07, 19 May 2021

OCA — Wed, 19 May 2021 10:07:46 GMT

←Older revision		Revision as of 10:07, 19 May 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7a74 is ~~ON HOLD until Paper Publication~~	+	==Structure of G132N BlaC from Mycobacterium tuberculosis==
-		+	<StructureSection load='7a74' size='340' side='right'caption='[[7a74]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A74 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a74 OCA], [https://pdbe.org/7a74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a74 RCSB], [https://www.ebi.ac.uk/pdbsum/7a74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a74 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ahmad MU]]
		+	[[Category: Chikunova A]]
		+	[[Category: Perrakis A]]
		+	[[Category: Ubbink M]]

OCA at 05:23, 24 February 2021

OCA — Wed, 24 Feb 2021 05:23:06 GMT

←Older revision		Revision as of 05:23, 24 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a74 is ON HOLD	+	The entry 7a74 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7a74" [edit=sysop:move=sysop]

OCA — Wed, 02 Sep 2020 05:07:18 GMT

Protected "7a74" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:07, 2 September 2020

OCA: New page: '''Unreleased structure''' The entry 7a74 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Sep 2020 05:07:17 GMT

New page: '''Unreleased structure''' The entry 7a74 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7a74 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]