7ac1 - Revision history

OCA at 11:10, 14 June 2023

OCA — Wed, 14 Jun 2023 11:10:13 GMT

←Older revision		Revision as of 11:10, 14 June 2023
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	==Solution structure of the TAF4-RST domain==		==Solution structure of the TAF4-RST domain==
-	<StructureSection load='7ac1' size='340' side='right'caption='[[7ac1~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='7ac1' size='340' side='right'caption='[[7ac1]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ac1]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ac1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AC1 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac1 OCA], [https://pdbe.org/7ac1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac1 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac1 OCA], [https://pdbe.org/7ac1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TAF4B_ARATH TAF4B_ARATH]] TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription.	+	[https://www.uniprot.org/uniprot/TAF4B_ARATH TAF4B_ARATH] TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bugge, K]]	+	[[Category: Bugge K]]
-	[[Category: Davidsen, R]]	+	[[Category: Davidsen R]]
-	[[Category: Kragelund~~, B B~~]]	+	[[Category: Kragelund BB]]
-	[[Category: Skriver, K]]	+	[[Category: Skriver K]]
-	[[Category: Staby, L]]	+	[[Category: Staby L]]
-	~~[[Category: Alpha-alpha hairpin]]~~	+
-	~~[[Category: Alpha-alpha hub]]~~	+
-	~~[[Category: General transcription]]~~	+
-	~~[[Category: Tfiid]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription factor binding~~]]	+

OCA at 10:19, 18 May 2022

OCA — Wed, 18 May 2022 10:19:58 GMT

←Older revision		Revision as of 10:19, 18 May 2022
Line 1:		Line 1:

	==Solution structure of the TAF4-RST domain==		==Solution structure of the TAF4-RST domain==
-	<StructureSection load='7ac1' size='340' side='right'caption='[[7ac1]]' scene=''>	+	<StructureSection load='7ac1' size='340' side='right'caption='[[7ac1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ac1]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AC1 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac1 OCA], [https://pdbe.org/7ac1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac1 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac1 OCA], [https://pdbe.org/7ac1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/TAF4B_ARATH TAF4B_ARATH]] TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Formation of transcription factor:coregulator complexes is a key step in transcriptional regulation, with coregulators having essential functions as hub nodes in molecular networks. How specificity and selectivity are maintained in these nodes remain open questions. In this work, we addressed specificity in transcriptional networks using complexes formed between transcription factors and alphaalpha-hubs, a group of hub domains defined by a common alphaalpha-hairpin secondary structure motif, as a model. Using NMR spectroscopy and binding thermodynamics, we analyzed the structure, dynamics, stability, and ligand-binding properties of the Arabidopsis thaliana RST domains from transcription initiation complex factor TAF4 and known binding partner RCD1, and the TAFH domain from human TAF4, allowing comparison across species, functions, and architectural contexts. While these alphaalpha-hubs shared the alphaalpha-hairpin motif, they differed in length and orientation of accessory helices as well as in their thermodynamic profiles of ligand binding. Whereas biologically relevant RCD1:ligand pairs displayed high affinity driven by enthalpy, TAF4:ligand interactions were entropy-driven and exhibited less binding-induced structuring. We additionally identified a thermal unfolding state with a structured core for all three domains, although the temperature sensitivity differed. Thermal stability studies suggested that initial unfolding of the RCD1-RST domain localized around Helix 1, lending this region structural malleability, while effects in TAF4-RST were more stochastic, suggesting variability in structural adaptability upon binding. Collectively, our results support a model in which hub structure, flexibility and binding thermodynamics all contribute to alphaalpha-hub:transcription factor binding specificity, a finding of general relevance to the understanding of coregulator:ligand interactions and interactome sizes.
		+
		+	alphaalpha-hub coregulator structure and flexibility determine transcription factor binding and selection in regulatory interactomes.,Friis Theisen F, Salladini E, Davidsen R, Jo Rasmussen C, Staby L, Kragelund BB, Skriver K J Biol Chem. 2022 Apr 19:101963. doi: 10.1016/j.jbc.2022.101963. PMID:35452682<ref>PMID:35452682</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ac1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transcription initiation factors 3D structures\|Transcription initiation factors 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bugge K]]	+	[[Category: Bugge, K]]
-	[[Category: Davidsen R]]	+	[[Category: Davidsen, R]]
-	[[Category: Kragelund BB]]	+	[[Category: Kragelund, B B]]
-	[[Category: Skriver K]]	+	[[Category: Skriver, K]]
-	[[Category: Staby L]]	+	[[Category: Staby, L]]
		+	[[Category: Alpha-alpha hairpin]]
		+	[[Category: Alpha-alpha hub]]
		+	[[Category: General transcription]]
		+	[[Category: Tfiid]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription factor binding]]

OCA at 05:27, 6 October 2021

OCA — Wed, 06 Oct 2021 05:27:27 GMT

←Older revision		Revision as of 05:27, 6 October 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ac1 is ~~ON HOLD until Paper Publication~~	+	==Solution structure of the TAF4-RST domain==
-		+	<StructureSection load='7ac1' size='340' side='right'caption='[[7ac1]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AC1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac1 OCA], [https://pdbe.org/7ac1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bugge K]]
		+	[[Category: Davidsen R]]
		+	[[Category: Kragelund BB]]
		+	[[Category: Skriver K]]
		+	[[Category: Staby L]]

OCA at 03:59, 14 October 2020

OCA — Wed, 14 Oct 2020 03:59:04 GMT

←Older revision		Revision as of 03:59, 14 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ac1 is ON HOLD	+	The entry 7ac1 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ac1" [edit=sysop:move=sysop]

OCA — Wed, 16 Sep 2020 06:44:15 GMT

Protected "7ac1" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 16 September 2020

OCA: New page: '''Unreleased structure''' The entry 7ac1 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Sep 2020 06:44:14 GMT

New page: '''Unreleased structure''' The entry 7ac1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ac1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]