OCA at 12:08, 1 February 2024

OCA — Thu, 01 Feb 2024 12:08:52 GMT

←Older revision		Revision as of 12:08, 1 February 2024
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-	====	+	==Protease Sapp1p from Candida parapsilosis in complex with KB32==
-	<StructureSection load='7age' size='340' side='right'caption='[[7age]]' scene=''>	+	<StructureSection load='7age' size='340' side='right'caption='[[7age]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7age]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AGE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7age FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7age OCA], [https://pdbe.org/7age PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7age RCSB], [https://www.ebi.ac.uk/pdbsum/7age PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7age ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOC:TERT-BUTYL+HYDROGEN+CARBONATE'>BOC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PSA:3-HYDROXY-4-AMINO-5-PHENYLPENTANOIC+ACID'>PSA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7age FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7age OCA], [https://pdbe.org/7age PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7age RCSB], [https://www.ebi.ac.uk/pdbsum/7age PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7age ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CARP1_CANPA CARP1_CANPA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pathogenic Candida albicans yeasts frequently cause infections in hospitals. Antifungal drugs lose effectiveness due to other Candida species and resistance. New medications are thus required. Secreted aspartic protease of C. parapsilosis (Sapp1p) is a promising target. We have thus solved the crystal structures of Sapp1p complexed to four peptidomimetic inhibitors. Three potent inhibitors (K(i): 0.1, 0.4, 6.6 nM) resembled pepstatin A (K(i): 0.3 nM), a general aspartic protease inhibitor, in terms of their interactions with Sapp1p. However, the weaker inhibitor (K(i): 14.6 nM) formed fewer nonpolar contacts with Sapp1p, similarly to the smaller HIV protease inhibitor ritonavir (K(i): 1.9 microM), which, moreover, formed fewer H-bonds. The analyses have revealed the structural determinants of the subnanomolar inhibition of C. parapsilosis aspartic protease. Because of the high similarity between Saps from different Candida species, these results can further be used for the design of potent and specific Sap inhibitor-based antimycotic drugs.
		+
		+	Structural determinants for subnanomolar inhibition of the secreted aspartic protease Sapp1p from Candida parapsilosis.,Dostal J, Brynda J, Vankova L, Zia SR, Pichova I, Heidingsfeld O, Lepsik M J Enzyme Inhib Med Chem. 2021 Dec;36(1):914-921. doi: , 10.1080/14756366.2021.1906664. PMID:33843395<ref>PMID:33843395</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7age" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candida parapsilosis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Brynda J]]
		+	[[Category: Dostal J]]
		+	[[Category: Heidingsfeld O]]

OCA at 08:21, 21 April 2021

OCA — Wed, 21 Apr 2021 08:21:23 GMT

←Older revision		Revision as of 08:21, 21 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7age ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7age' size='340' side='right'caption='[[7age]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7age FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7age OCA], [https://pdbe.org/7age PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7age RCSB], [https://www.ebi.ac.uk/pdbsum/7age PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7age ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7age" [edit=sysop:move=sysop]

OCA — Wed, 28 Oct 2020 18:51:36 GMT

Protected "7age" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:51, 28 October 2020

OCA: New page: '''Unreleased structure''' The entry 7age is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Oct 2020 18:51:35 GMT

New page: '''Unreleased structure''' The entry 7age is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7age is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7age - Revision history

OCA at 12:08, 1 February 2024

OCA at 08:21, 21 April 2021

OCA: Protected "7age" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7age is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures