7aht - Revision history

OCA at 12:09, 1 February 2024

OCA — Thu, 01 Feb 2024 12:09:38 GMT

←Older revision		Revision as of 12:09, 1 February 2024
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	<StructureSection load='7aht' size='340' side='right'caption='[[7aht]], [[Resolution\|resolution]] 2.16Å' scene=''>		<StructureSection load='7aht' size='340' side='right'caption='[[7aht]], [[Resolution\|resolution]] 2.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7aht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7aht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AHT FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[7agv\|7agv]], [[7agy\|7agy]], [[7agw\|7agw]], [[7ahm\|7ahm]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.16Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">khtT, yhaT, BSU09860 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=224308 BACSU])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aht OCA], [https://pdbe.org/7aht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aht RCSB], [https://www.ebi.ac.uk/pdbsum/7aht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aht ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aht OCA], [https://pdbe.org/7aht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aht RCSB], [https://www.ebi.ac.uk/pdbsum/7aht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aht ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/KHTT_BACSU KHTT_BACSU]] Required for activity of the potassium/proton antiporter KhtU (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).<ref>PMID:14987767</ref> <ref>PMID:17679694</ref> <ref>PMID:24330391</ref>	+	[https://www.uniprot.org/uniprot/KHTT_BACSU KHTT_BACSU] Required for activity of the potassium/proton antiporter KhtU (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).<ref>PMID:14987767</ref> <ref>PMID:17679694</ref> <ref>PMID:24330391</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cereija~~, T B~~]]	+	[[Category: Cereija TB]]
-	[[Category: Morais-Cabral~~, J H]]~~	+	[[Category: Morais-Cabral JH]]
-	~~[[Category: Regulatory protein of k+/h+ antiporter]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 06:56, 14 April 2021

OCA — Wed, 14 Apr 2021 06:56:35 GMT

←Older revision		Revision as of 06:56, 14 April 2021
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/KHTT_BACSU KHTT_BACSU]] Required for activity of the potassium/proton antiporter KhtU (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).<ref>PMID:14987767</ref> <ref>PMID:17679694</ref> <ref>PMID:24330391</ref>		[[https://www.uniprot.org/uniprot/KHTT_BACSU KHTT_BACSU]] Required for activity of the potassium/proton antiporter KhtU (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).<ref>PMID:14987767</ref> <ref>PMID:17679694</ref> <ref>PMID:24330391</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	bis-(3',5')-cyclic diadenosine monophosphate (c-di-AMP) is a second messenger with roles in virulence, cell wall and biofilm formation, and surveillance of DNA integrity in many bacterial species, including pathogens. Strikingly, it has also been proposed to coordinate the activity of the components of K(+) homeostasis machinery, inhibiting K(+) import, and activating K(+) export. However, there is a lack of quantitative evidence supporting the direct functional impact of c-di-AMP on K(+) transporters. To gain a detailed understanding of the role of c-di-AMP on the activity of a component of the K(+) homeostasis machinery in B. subtilis, we have characterized the impact of c-di-AMP on the functional, biochemical, and physiological properties of KhtTU, a K(+)/H(+) antiporter composed of the membrane protein KhtU and the cytosolic protein KhtT. We have confirmed c-di-AMP binding to KhtT and determined the crystal structure of this complex. We have characterized in vitro the functional properties of KhtTU and KhtU alone and quantified the impact of c-di-AMP and of pH on their activity, demonstrating that c-di-AMP activates KhtTU and that pH increases its sensitivity to this nucleotide. Based on our functional and structural data, we were able to propose a mechanism for the activation of KhtTU by c-di-AMP. In addition, we have analyzed the impact of KhtTU in its native bacterium, providing a physiological context for the regulatory function of c-di-AMP and pH. Overall, we provide unique information that supports the proposal that c-di-AMP is a master regulator of K+ homeostasis machinery.
		+
		+	c-di-AMP, a likely master regulator of bacterial K(+) homeostasis machinery, activates a K(+) exporter.,Cereija TB, Guerra JPL, Jorge JMP, Morais-Cabral JH Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2020653118. doi:, 10.1073/pnas.2020653118. PMID:33790011<ref>PMID:33790011</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7aht" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:08, 7 April 2021

OCA — Wed, 07 Apr 2021 07:08:05 GMT

←Older revision		Revision as of 07:08, 7 April 2021
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	==Structure of the N-domain of the K+/H+ antiporter subunit KhtT at pH 7.5==		==Structure of the N-domain of the K+/H+ antiporter subunit KhtT at pH 7.5==
-	<StructureSection load='7aht' size='340' side='right'caption='[[7aht]]' scene=''>	+	<StructureSection load='7aht' size='340' side='right'caption='[[7aht]], [[Resolution\|resolution]] 2.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7aht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aht OCA], [https://pdbe.org/7aht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aht RCSB], [https://www.ebi.ac.uk/pdbsum/7aht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aht ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7agv\|7agv]], [[7agy\|7agy]], [[7agw\|7agw]], [[7ahm\|7ahm]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">khtT, yhaT, BSU09860 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aht OCA], [https://pdbe.org/7aht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aht RCSB], [https://www.ebi.ac.uk/pdbsum/7aht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aht ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/KHTT_BACSU KHTT_BACSU]] Required for activity of the potassium/proton antiporter KhtU (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).<ref>PMID:14987767</ref> <ref>PMID:17679694</ref> <ref>PMID:24330391</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacsu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cereija TB]]	+	[[Category: Cereija, T B]]
-	[[Category: Morais-Cabral JH]]	+	[[Category: Morais-Cabral, J H]]
		+	[[Category: Regulatory protein of k+/h+ antiporter]]
		+	[[Category: Transport protein]]

OCA at 10:31, 31 March 2021

OCA — Wed, 31 Mar 2021 10:31:09 GMT

←Older revision		Revision as of 10:31, 31 March 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7aht ~~is ON HOLD until Paper Publication~~	+	==Structure of the N-domain of the K+/H+ antiporter subunit KhtT at pH 7.5==
-		+	<StructureSection load='7aht' size='340' side='right'caption='[[7aht]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AHT FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aht OCA], [https://pdbe.org/7aht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aht RCSB], [https://www.ebi.ac.uk/pdbsum/7aht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aht ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cereija TB]]
		+	[[Category: Morais-Cabral JH]]

OCA at 05:17, 4 November 2020

OCA — Wed, 04 Nov 2020 05:17:15 GMT

←Older revision		Revision as of 05:17, 4 November 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7aht is ON HOLD	+	The entry 7aht is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7aht" [edit=sysop:move=sysop]

OCA — Wed, 07 Oct 2020 04:23:22 GMT

Protected "7aht" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:23, 7 October 2020

OCA: New page: '''Unreleased structure''' The entry 7aht is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Oct 2020 04:23:20 GMT

New page: '''Unreleased structure''' The entry 7aht is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7aht is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]