7ai3 - Revision history

OCA at 07:44, 1 May 2024

OCA — Wed, 01 May 2024 07:44:48 GMT

←Older revision		Revision as of 07:44, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='7ai3' size='340' side='right'caption='[[7ai3]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='7ai3' size='340' side='right'caption='[[7ai3]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ai3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AI3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ai3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AI3 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[7ai2\|7ai2]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">mce4A, Rv3499c ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83332 MYCTU])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ai3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ai3 OCA], [https://pdbe.org/7ai3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ai3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ai3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ai3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ai3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ai3 OCA], [https://pdbe.org/7ai3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ai3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ai3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ai3 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/I6YC99_MYCTU I6YC99_MYCTU]
-	Mycobacterium tuberculosis (Mtb), which is responsible for more than a million deaths annually, uses lipids as the source of carbon and energy for its survival in the latent phase of infection. Mtb cannot synthesize all of the lipid molecules required for its growth and pathogenicity. Therefore, it relies on transporters such as the mammalian cell entry (Mce) complexes to import lipids from the host across the cell wall. Despite their importance for the survival and pathogenicity of Mtb, information on the structural properties of these proteins is not yet available. Each of the four Mce complexes in Mtb (Mce1-4) comprises six substrate-binding proteins (SBPs; MceA-F), each of which contains four conserved domains (N-terminal transmembrane, MCE, helical and C-terminal unstructured tail domains). Here, the properties of the various domains of Mtb Mce1A and Mce4A, which are involved in the import of mycolic/fatty acids and cholesterol, respectively, are reported. In the crystal structure of the MCE domain of Mce4A (MtMce4A39-140) a domain-swapped conformation is observed, whereas solution studies, including small-angle X-ray scattering (SAXS), indicate that all Mce1A and Mce4A domains are predominantly monomeric. Further, structural comparisons show interesting differences from the bacterial homologs MlaD, PqiB and LetB, which form homohexamers when assembled as functional transporter complexes. These data, and the fact that there are six SBPs in each Mtb mce operon, suggest that the MceA-F SBPs from Mce1-4 may form heterohexamers. Also, interestingly, the purification and SAXS analysis showed that the helical domains interact with the detergent micelle, suggesting that when assembled the helical domains of MceA-F may form a hydrophobic pore for lipid transport, as observed in EcPqiB. Overall, these data highlight the unique structural properties of the Mtb Mce SBPs.	+
-		+
-	Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis.,Asthana P, Singh D, Pedersen JS, Hynonen MJ, Sulu R, Murthy AV, Laitaoja M, Janis J, Riley LW, Venkatesan R IUCrJ. 2021 Jul 28;8(Pt 5):~~757-774. doi: 10.1107~~/~~S2052252521006199. eCollection, 2021 Sep 1. PMID:34584737<ref>PMID:34584737<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 7ai3" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Asthana, P]]	+	[[Category: Asthana P]]
-	[[Category: Venkatesan, R]]	+	[[Category: Venkatesan R]]
-	~~[[Category: Mce]]~~	+
-	~~[[Category: Mce4a]]~~	+
-	~~[[Category: Substrate binding protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 12:47, 13 October 2021

OCA — Wed, 13 Oct 2021 12:47:28 GMT

←Older revision		Revision as of 12:47, 13 October 2021
Line 1:		Line 1:

	==Crystal structure of MCE domain of Mce4A from Mycobacterium tuberculosis H37Rv==		==Crystal structure of MCE domain of Mce4A from Mycobacterium tuberculosis H37Rv==
-	<StructureSection load='7ai3' size='340' side='right'caption='[[7ai3]]' scene=''>	+	<StructureSection load='7ai3' size='340' side='right'caption='[[7ai3]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AI3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ai3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AI3 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ai3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ai3 OCA], [https://pdbe.org/7ai3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ai3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ai3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ai3 ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7ai2\|7ai2]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mce4A, Rv3499c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ai3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ai3 OCA], [https://pdbe.org/7ai3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ai3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ai3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ai3 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacterium tuberculosis (Mtb), which is responsible for more than a million deaths annually, uses lipids as the source of carbon and energy for its survival in the latent phase of infection. Mtb cannot synthesize all of the lipid molecules required for its growth and pathogenicity. Therefore, it relies on transporters such as the mammalian cell entry (Mce) complexes to import lipids from the host across the cell wall. Despite their importance for the survival and pathogenicity of Mtb, information on the structural properties of these proteins is not yet available. Each of the four Mce complexes in Mtb (Mce1-4) comprises six substrate-binding proteins (SBPs; MceA-F), each of which contains four conserved domains (N-terminal transmembrane, MCE, helical and C-terminal unstructured tail domains). Here, the properties of the various domains of Mtb Mce1A and Mce4A, which are involved in the import of mycolic/fatty acids and cholesterol, respectively, are reported. In the crystal structure of the MCE domain of Mce4A (MtMce4A39-140) a domain-swapped conformation is observed, whereas solution studies, including small-angle X-ray scattering (SAXS), indicate that all Mce1A and Mce4A domains are predominantly monomeric. Further, structural comparisons show interesting differences from the bacterial homologs MlaD, PqiB and LetB, which form homohexamers when assembled as functional transporter complexes. These data, and the fact that there are six SBPs in each Mtb mce operon, suggest that the MceA-F SBPs from Mce1-4 may form heterohexamers. Also, interestingly, the purification and SAXS analysis showed that the helical domains interact with the detergent micelle, suggesting that when assembled the helical domains of MceA-F may form a hydrophobic pore for lipid transport, as observed in EcPqiB. Overall, these data highlight the unique structural properties of the Mtb Mce SBPs.
		+
		+	Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis.,Asthana P, Singh D, Pedersen JS, Hynonen MJ, Sulu R, Murthy AV, Laitaoja M, Janis J, Riley LW, Venkatesan R IUCrJ. 2021 Jul 28;8(Pt 5):757-774. doi: 10.1107/S2052252521006199. eCollection, 2021 Sep 1. PMID:34584737<ref>PMID:34584737</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ai3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Asthana P]]	+	[[Category: Myctu]]
-	[[Category: Venkatesan R]]	+	[[Category: Asthana, P]]
		+	[[Category: Venkatesan, R]]
		+	[[Category: Mce]]
		+	[[Category: Mce4a]]
		+	[[Category: Substrate binding protein]]
		+	[[Category: Transport protein]]

OCA at 06:17, 25 August 2021

OCA — Wed, 25 Aug 2021 06:17:21 GMT

←Older revision		Revision as of 06:17, 25 August 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ai3 ~~is ON HOLD until Sep 25 2022~~	+	==Crystal structure of MCE domain of Mce4A from Mycobacterium tuberculosis H37Rv==
-		+	<StructureSection load='7ai3' size='340' side='right'caption='[[7ai3]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AI3 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ai3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ai3 OCA], [https://pdbe.org/7ai3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ai3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ai3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ai3 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Asthana P]]
		+	[[Category: Venkatesan R]]

OCA at 04:01, 14 October 2020

OCA — Wed, 14 Oct 2020 04:01:47 GMT

←Older revision		Revision as of 04:01, 14 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ai3 is ON HOLD	+	The entry 7ai3 is ON HOLD until Sep 25 2022

	Authors:		Authors:

OCA: Protected "7ai3" [edit=sysop:move=sysop]

OCA — Wed, 07 Oct 2020 04:24:01 GMT

Protected "7ai3" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:24, 7 October 2020

OCA: New page: '''Unreleased structure''' The entry 7ai3 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Oct 2020 04:24:00 GMT

New page: '''Unreleased structure''' The entry 7ai3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ai3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]