7aoy - Revision history

OCA at 07:06, 18 May 2023

OCA — Thu, 18 May 2023 07:06:04 GMT

←Older revision		Revision as of 07:06, 18 May 2023
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	==Helical arrangement of Bunyamwera virus nucleocapsid protein within a native ribonucleoprotein==		==Helical arrangement of Bunyamwera virus nucleocapsid protein within a native ribonucleoprotein==
-	<StructureSection load='7aoy' size='340' side='right'caption='[[7aoy]]' scene=''>	+	<StructureSection load='7aoy' size='340' side='right'caption='[[7aoy]], [[Resolution\|resolution]] 13.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AOY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7aoy]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Bunyamwera_virus Bunyamwera virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AOY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aoy OCA], [https://pdbe.org/7aoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aoy RCSB], [https://www.ebi.ac.uk/pdbsum/7aoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aoy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aoy OCA], [https://pdbe.org/7aoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aoy RCSB], [https://www.ebi.ac.uk/pdbsum/7aoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aoy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NCAP_BUNYW NCAP_BUNYW]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Bunyavirales order is the largest group of negative-sense RNA viruses, containing many lethal human pathogens for which approved anti-infective measures are not available. The bunyavirus genome consists of multiple negative-sense RNA segments enwrapped by the virus-encoded nucleocapsid protein (NP), which together with the viral polymerase form ribonucleoproteins (RNPs). RNPs represent substrates for RNA synthesis and virion assembly, which require inherent flexibility, consistent with the appearance of RNPs spilled from virions. These observations have resulted in conflicting models describing the overall RNP architecture. Here, we purified RNPs from Bunyamwera virus (BUNV), the prototypical orthobunyavirus. The lengths of purified RNPs imaged by negative staining resulted in 3 populations of RNPs, suggesting that RNPs possess a consistent method of condensation. Employing microscopy approaches, we conclusively show that the NP portion of BUNV RNPs is helical. Furthermore, we present a pseudo-atomic model for this portion based on a cryo-electron microscopy average at 13 A resolution, which allowed us to fit the BUNV NP crystal structure by molecular dynamics. This model was confirmed by NP mutagenesis using a mini-genome system. The model shows that adjacent NP monomers in the RNP chain interact laterally through flexible N- and C-terminal arms only, with no longitudinal helix-stabilizing interactions, thus providing a potential model for the molecular basis for RNP flexibility. Excessive RNase treatment disrupts native RNPs, suggesting that RNA was key in maintaining the RNP structure. Overall, this work will inform studies on bunyaviral RNP assembly, packaging, and RNA replication, and aid in future antiviral strategies. IMPORTANCE Bunyaviruses are emerging RNA viruses that cause significant disease and economic burden and for which vaccines or therapies approved for humans are not available. The bunyavirus genome is wrapped up by the nucleoprotein (NP) and interacts with the viral polymerase, forming a ribonucleoprotein (RNP). This is the only form of the genome active for viral replication and assembly. However, until now how NPs are organized within an RNP was not known for any orthobunyavirus. Here, we purified RNPs from the prototypical orthobunyavirus, Bunyamwera virus, and employed microscopy approaches to show that the NP portion of the RNP was helical. We then combined our helical average with the known structure of an NP monomer, generating a pseudo-atomic model of this region. This arrangement allowed the RNPs to be highly flexible, which was critical for several stages of the viral replication cycle, such as segment circularization.
		+
		+	The Native Orthobunyavirus Ribonucleoprotein Possesses a Helical Architecture.,Hopkins FR, Alvarez-Rodriguez B, Heath GR, Panayi K, Hover S, Edwards TA, Barr JN, Fontana J mBio. 2022 Aug 30;13(4):e0140522. doi: 10.1128/mbio.01405-22. Epub 2022 Jun 28. PMID:35762594<ref>PMID:35762594</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7aoy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bunyamwera virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Barr JN]]		[[Category: Barr JN]]
	[[Category: Fontana J]]		[[Category: Fontana J]]
	[[Category: Hopkins FR]]		[[Category: Hopkins FR]]

OCA at 12:46, 4 May 2022

OCA — Wed, 04 May 2022 12:46:00 GMT

←Older revision		Revision as of 12:46, 4 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7aoy ~~is ON HOLD until Paper Publication~~	+	==Helical arrangement of Bunyamwera virus nucleocapsid protein within a native ribonucleoprotein==
-		+	<StructureSection load='7aoy' size='340' side='right'caption='[[7aoy]]' scene=''>
-	~~Authors~~: ~~Hopkins~~, F.R., ~~Fontana~~, J., ~~Barr~~, J.N.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AOY FirstGlance]. <br>
-	~~Description: Helical arrangement of Bunyamwera virus nucleocapsid protein within a native ribonucleoprotein~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aoy OCA], [https://pdbe.org/7aoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aoy RCSB], [https://www.ebi.ac.uk/pdbsum/7aoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aoy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Fontana, J~~]]	+	__TOC__
-	[[Category: ~~Hopkins, F.R~~]]	+	</StructureSection>
-	[[Category: ~~Barr, J.N~~]]	+	[[Category: Large Structures]]
		+	[[Category: Barr JN]]
		+	[[Category: Fontana J]]
		+	[[Category: Hopkins FR]]

OCA at 04:57, 9 December 2020

OCA — Wed, 09 Dec 2020 04:57:02 GMT

←Older revision		Revision as of 04:57, 9 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7aoy is ON HOLD	+	The entry 7aoy is ON HOLD until Paper Publication

	Authors: Hopkins, F.R., Fontana, J., Barr, J.N.		Authors: Hopkins, F.R., Fontana, J., Barr, J.N.
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	Description: Helical arrangement of Bunyamwera virus nucleocapsid protein within a native ribonucleoprotein		Description: Helical arrangement of Bunyamwera virus nucleocapsid protein within a native ribonucleoprotein
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Barr, J.N]]
-	[[Category: Hopkins, F.R]]
	[[Category: Fontana, J]]		[[Category: Fontana, J]]
		+	[[Category: Hopkins, F.R]]
		+	[[Category: Barr, J.N]]

OCA: Protected "7aoy" [edit=sysop:move=sysop]

OCA — Wed, 21 Oct 2020 06:40:23 GMT

Protected "7aoy" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:40, 21 October 2020

OCA: New page: '''Unreleased structure''' The entry 7aoy is ON HOLD Authors: Hopkins, F.R., Fontana, J., Barr, J.N. Description: Helical arrangement of Bunyamwera virus nucleocapsid protein within a ...

OCA — Wed, 21 Oct 2020 06:40:21 GMT

New page: '''Unreleased structure''' The entry 7aoy is ON HOLD Authors: Hopkins, F.R., Fontana, J., Barr, J.N. Description: Helical arrangement of Bunyamwera virus nucleocapsid protein within a ...

New page

'''Unreleased structure'''

The entry 7aoy is ON HOLD

Authors: Hopkins, F.R., Fontana, J., Barr, J.N.

Description: Helical arrangement of Bunyamwera virus nucleocapsid protein within a native ribonucleoprotein
[[Category: Unreleased Structures]]
[[Category: Barr, J.N]]
[[Category: Hopkins, F.R]]
[[Category: Fontana, J]]