7aug - Revision history

OCA at 12:16, 1 February 2024

OCA — Thu, 01 Feb 2024 12:16:19 GMT

←Older revision		Revision as of 12:16, 1 February 2024
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	<StructureSection load='7aug' size='340' side='right'caption='[[7aug]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='7aug' size='340' side='right'caption='[[7aug]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7aug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7aug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.04Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aug OCA], [https://pdbe.org/7aug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aug RCSB], [https://www.ebi.ac.uk/pdbsum/7aug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aug ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aug OCA], [https://pdbe.org/7aug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aug RCSB], [https://www.ebi.ac.uk/pdbsum/7aug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aug ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of CPVT4. The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of LQT14.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).<ref>PMID:16760425</ref> <ref>PMID:23893133</ref> <ref>PMID:26969752</ref> <ref>PMID:27165696</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fuenzalida-Werner~~, J P~~]]	+	[[Category: Fuenzalida-Werner JP]]
-	[[Category: Janowski, R]]	+	[[Category: Janowski R]]
-	[[Category: Mishra, K]]	+	[[Category: Mishra K]]
-	[[Category: Niessing, D]]	+	[[Category: Niessing D]]
-	[[Category: Stiel~~, A C]]~~	+	[[Category: Stiel AC]]
-	~~[[Category: Calcium sensor]]~~	+
-	~~[[Category: Calmodulin]]~~	+
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Reversible switchable fluorescent protein~~]]	+

OCA at 15:34, 22 December 2021

OCA — Wed, 22 Dec 2021 15:34:51 GMT

←Older revision		Revision as of 15:34, 22 December 2021
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	==Crystal structure of rsGCamP1.3 in the ON state==		==Crystal structure of rsGCamP1.3 in the ON state==
-	<StructureSection load='7aug' size='340' side='right'caption='[[7aug]]' scene=''>	+	<StructureSection load='7aug' size='340' side='right'caption='[[7aug]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7aug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aug OCA], [https://pdbe.org/7aug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aug RCSB], [https://www.ebi.ac.uk/pdbsum/7aug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aug ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aug OCA], [https://pdbe.org/7aug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aug RCSB], [https://www.ebi.ac.uk/pdbsum/7aug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aug ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Reversibly photo-switchable proteins are essential for many super-resolution fluorescence microscopic and optoacoustic imaging methods. However, they have yet to be used as sensors that measure the distribution of specific analytes at the nanoscale or in the tissues of live animals. Here we constructed the prototype of a photo-switchable Ca(2+) sensor based on GCaMP5G that can be switched with 405/488-nm light and describe its molecular mechanisms at the structural level, including the importance of the interaction of the core barrel structure of the fluorescent protein with the Ca(2+) receptor moiety. We demonstrate super-resolution imaging of Ca(2+) concentration in cultured cells and optoacoustic Ca(2+) imaging in implanted tumor cells in mice under controlled Ca(2+) conditions. Finally, we show the generalizability of the concept by constructing examples of photo-switching maltose and dopamine sensors based on periplasmatic binding protein and G-protein-coupled receptor-based sensors.
		+
		+	Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.,Mishra K, Fuenzalida-Werner JP, Pennacchietti F, Janowski R, Chmyrov A, Huang Y, Zakian C, Klemm U, Testa I, Niessing D, Ntziachristos V, Stiel AC Nat Biotechnol. 2021 Nov 29. pii: 10.1038/s41587-021-01100-5. doi:, 10.1038/s41587-021-01100-5. PMID:34845372<ref>PMID:34845372</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7aug" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fuenzalida-Werner JP]]	+	[[Category: Fuenzalida-Werner, J P]]
-	[[Category: Janowski R]]	+	[[Category: Janowski, R]]
-	[[Category: Mishra K]]	+	[[Category: Mishra, K]]
-	[[Category: Niessing D]]	+	[[Category: Niessing, D]]
-	[[Category: Stiel AC]]	+	[[Category: Stiel, A C]]
		+	[[Category: Calcium sensor]]
		+	[[Category: Calmodulin]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Reversible switchable fluorescent protein]]

OCA at 14:09, 27 October 2021

OCA — Wed, 27 Oct 2021 14:09:23 GMT

←Older revision		Revision as of 14:09, 27 October 2021
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-	'''Unreleased structure'''

-	~~The entry 7aug is~~ ON ~~HOLD until Paper Publication~~	+	==Crystal structure of rsGCamP1.3 in the ON state==
-		+	<StructureSection load='7aug' size='340' side='right'caption='[[7aug]]' scene=''>
-	~~Authors~~: ~~Janowski, R~~.~~, Fuenzalida~~-~~Werner~~, J.P., ~~Mishra~~, K., ~~Stiel, A~~.C., ~~Niessing, D~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUG FirstGlance]. <br>
-	~~Description: Crystal structure of rsGCamP1.3 in the ON state~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aug OCA], [https://pdbe.org/7aug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aug RCSB], [https://www.ebi.ac.uk/pdbsum/7aug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aug ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Niessing, D~~]]	+	__TOC__
-	[[Category: Janowski, R]]	+	</StructureSection>
-	[[Category: ~~Fuenzalida-Werner, J.P~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Stiel, A.C~~]]	+	[[Category: Fuenzalida-Werner JP]]
-	[[Category: ~~Mishra, K~~]]	+	[[Category: Janowski R]]
		+	[[Category: Mishra K]]
		+	[[Category: Niessing D]]
		+	[[Category: Stiel AC]]

OCA at 05:29, 2 December 2020

OCA — Wed, 02 Dec 2020 05:29:49 GMT

←Older revision		Revision as of 05:29, 2 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7aug is ON HOLD	+	The entry 7aug is ON HOLD until Paper Publication

	Authors: Janowski, R., Fuenzalida-Werner, J.P., Mishra, K., Stiel, A.C., Niessing, D.		Authors: Janowski, R., Fuenzalida-Werner, J.P., Mishra, K., Stiel, A.C., Niessing, D.
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	Description: Crystal structure of rsGCamP1.3 in the ON state		Description: Crystal structure of rsGCamP1.3 in the ON state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Mishra, K]]
-	[[Category: Stiel, A.C]]
	[[Category: Niessing, D]]		[[Category: Niessing, D]]
-	[[Category: Fuenzalida-Werner, J.P]]
	[[Category: Janowski, R]]		[[Category: Janowski, R]]
		+	[[Category: Fuenzalida-Werner, J.P]]
		+	[[Category: Stiel, A.C]]
		+	[[Category: Mishra, K]]

OCA: Protected "7aug" [edit=sysop:move=sysop]

OCA — Wed, 11 Nov 2020 06:17:47 GMT

Protected "7aug" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:17, 11 November 2020

OCA: New page: '''Unreleased structure''' The entry 7aug is ON HOLD Authors: Janowski, R., Fuenzalida-Werner, J.P., Mishra, K., Stiel, A.C., Niessing, D. Description: Crystal structure of rsGCamP1.3 ...

OCA — Wed, 11 Nov 2020 06:17:46 GMT

New page: '''Unreleased structure''' The entry 7aug is ON HOLD Authors: Janowski, R., Fuenzalida-Werner, J.P., Mishra, K., Stiel, A.C., Niessing, D. Description: Crystal structure of rsGCamP1.3 ...

New page

'''Unreleased structure'''

The entry 7aug is ON HOLD

Authors: Janowski, R., Fuenzalida-Werner, J.P., Mishra, K., Stiel, A.C., Niessing, D.

Description: Crystal structure of rsGCamP1.3 in the ON state
[[Category: Unreleased Structures]]
[[Category: Mishra, K]]
[[Category: Stiel, A.C]]
[[Category: Niessing, D]]
[[Category: Fuenzalida-Werner, J.P]]
[[Category: Janowski, R]]