OCA at 12:16, 1 February 2024

OCA — Thu, 01 Feb 2024 12:16:21 GMT

←Older revision		Revision as of 12:16, 1 February 2024
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	==Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1 in complex with InsP6==		==Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1 in complex with InsP6==
-	<StructureSection load='7aui' size='340' side='right'caption='[[7aui]]' scene=''>	+	<StructureSection load='7aui' size='340' side='right'caption='[[7aui]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7aui]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aui OCA], [https://pdbe.org/7aui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aui RCSB], [https://www.ebi.ac.uk/pdbsum/7aui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aui ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.98Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aui OCA], [https://pdbe.org/7aui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aui RCSB], [https://www.ebi.ac.uk/pdbsum/7aui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aui ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DDP1_YEAST DDP1_YEAST] May eliminate potentially toxic dinucleoside polyphosphates during sporulation. Most active against diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A). Can also hydrolyze diadenosine 5',5'''-P1,P5-pentaphosphate (Ap5A), adenosine 5'-pentaphosphate, and adenosine 5'-tetraphosphate are also substrates, but not diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols. Also cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The yeast diadenosine and diphosphoinositol polyphosphate phosphohydrolase DDP1 is a Nudix enzyme with pyrophosphatase activity on diphosphoinositides, dinucleotides, and polyphosphates. These substrates bind to diverse protein targets and participate in signaling and metabolism, being essential for energy and phosphate homeostasis, ATPase pump regulation, or protein phosphorylation. An exhaustive structural study of DDP1 in complex with multiple ligands related to its three diverse substrate classes is reported. This allowed full characterization of the DDP1 active site depicting the molecular basis for endowing multisubstrate abilities to a Nudix enzyme, driven by phosphate anchoring following a defined path. This study, combined with multiple enzyme variants, reveals the different substrate binding modes, preferences, and selection. Our findings expand current knowledge on this important structural superfamily with implications extending beyond inositide research. This work represents a valuable tool for inhibitor/substrate design for ScDDP1 and orthologs as potential targets to address fungal infections and other health concerns.
		+
		+	Multiple substrate recognition by yeast diadenosine and diphosphoinositol polyphosphate phosphohydrolase through phosphate clamping.,Marquez-Monino MA, Ortega-Garcia R, Shipton ML, Franco-Echevarria E, Riley AM, Sanz-Aparicio J, Potter BVL, Gonzalez B Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabf6744. doi: 10.1126/sciadv.abf6744., Print 2021 Apr. PMID:33893105<ref>PMID:33893105</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7aui" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae S288C]]
	[[Category: Gonzalez B]]		[[Category: Gonzalez B]]
	[[Category: Marquez-Monino MA]]		[[Category: Marquez-Monino MA]]

OCA at 10:07, 19 May 2021

OCA — Wed, 19 May 2021 10:07:55 GMT

←Older revision		Revision as of 10:07, 19 May 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7aui ~~is ON HOLD until Paper Publication~~	+	==Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1 in complex with InsP6==
-		+	<StructureSection load='7aui' size='340' side='right'caption='[[7aui]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AUI FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aui OCA], [https://pdbe.org/7aui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aui RCSB], [https://www.ebi.ac.uk/pdbsum/7aui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aui ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gonzalez B]]
		+	[[Category: Marquez-Monino MA]]

OCA at 09:11, 31 March 2021

OCA — Wed, 31 Mar 2021 09:11:50 GMT

←Older revision		Revision as of 09:11, 31 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7aui is ON HOLD	+	The entry 7aui is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7aui" [edit=sysop:move=sysop]

OCA — Wed, 11 Nov 2020 06:17:56 GMT

Protected "7aui" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:17, 11 November 2020

OCA: New page: '''Unreleased structure''' The entry 7aui is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 11 Nov 2020 06:17:55 GMT

New page: '''Unreleased structure''' The entry 7aui is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7aui is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7aui - Revision history

OCA at 12:16, 1 February 2024

OCA at 10:07, 19 May 2021

OCA at 09:11, 31 March 2021

OCA: Protected "7aui" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7aui is ON HOLD Authors: Description: Category: Unreleased Structures