7b3u - Revision history

OCA at 10:54, 15 November 2023

OCA — Wed, 15 Nov 2023 10:54:38 GMT

←Older revision		Revision as of 10:54, 15 November 2023
Line 1:		Line 1:

	==OXA-10 beta-lactamase with covalent modification==		==OXA-10 beta-lactamase with covalent modification==
-	<StructureSection load='7b3u' size='340' side='right'caption='[[7b3u]]' scene=''>	+	<StructureSection load='7b3u' size='340' side='right'caption='[[7b3u]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7b3u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3u OCA], [https://pdbe.org/7b3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3u RCSB], [https://www.ebi.ac.uk/pdbsum/7b3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3u ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.598Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SWW:(2~{S})-2-azanyl-3-(2-hydroxyethylsulfanyl)propanoic+acid'>SWW</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3u OCA], [https://pdbe.org/7b3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3u RCSB], [https://www.ebi.ac.uk/pdbsum/7b3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI] Hydrolyzes both carbenicillin and oxacillin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SignificanceMicrobial resistance to beta-lactam antibiotics mediated by beta-lactamase-catalyzed hydrolysis is a major global health concern. Penam sulfones, which are structurally related to penicillins, inhibit clinically important serine beta-lactamases (SBLs) by forming transiently stable covalent complexes, thereby protecting beta-lactam antibiotics from hydrolysis. The characterization of these complexes and mechanisms of SBL inhibition is important for development of new SBL inhibitors (SBLi). Studies on the mechanism of the new SBLi enmetazobactam employing mass spectrometry and X-ray crystallography inform on its mode of action and also lead to reevaluation of mechanisms of current clinically important SBLi. In addition to insights into the mechanisms of transient SBL inhibition by penam sulfones, the results reveal potential for penam sulfone optimization to enable irreversible SBL inhibition.
		+
		+	Studies on enmetazobactam clarify mechanisms of widely used beta-lactamase inhibitors.,Lang PA, Raj R, Tumber A, Lohans CT, Rabe P, Robinson CV, Brem J, Schofield CJ Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2117310119. doi:, 10.1073/pnas.2117310119. Epub 2022 Apr 29. PMID:35486701<ref>PMID:35486701</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7b3u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa]]
	[[Category: Brem J]]		[[Category: Brem J]]
	[[Category: Lang PA]]		[[Category: Lang PA]]
	[[Category: Schofield CJ]]		[[Category: Schofield CJ]]

OCA at 14:43, 31 August 2022

OCA — Wed, 31 Aug 2022 14:43:05 GMT

←Older revision		Revision as of 14:43, 31 August 2022
Line 1:		Line 1:

	==OXA-10 beta-lactamase with covalent modification==		==OXA-10 beta-lactamase with covalent modification==
-	<StructureSection load='7b3u' size='340' side='right'caption='[[7b3u]]~~, [[Resolution\|resolution]] 1.60Å~~' scene=''>	+	<StructureSection load='7b3u' size='340' side='right'caption='[[7b3u]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7b3u]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3U FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3u OCA], [https://pdbe.org/7b3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3u RCSB], [https://www.ebi.ac.uk/pdbsum/7b3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3u ProSAT]</span></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWW:'>SWW</scene></td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3u OCA], [https://pdbe.org/7b3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3u RCSB], [https://www.ebi.ac.uk/pdbsum/7b3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3u ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI]] Hydrolyzes both carbenicillin and oxacillin.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	SignificanceMicrobial resistance to beta-lactam antibiotics mediated by beta-lactamase-catalyzed hydrolysis is a major global health concern. Penam sulfones, which are structurally related to penicillins, inhibit clinically important serine beta-lactamases (SBLs) by forming transiently stable covalent complexes, thereby protecting beta-lactam antibiotics from hydrolysis. The characterization of these complexes and mechanisms of SBL inhibition is important for development of new SBL inhibitors (SBLi). Studies on the mechanism of the new SBLi enmetazobactam employing mass spectrometry and X-ray crystallography inform on its mode of action and also lead to reevaluation of mechanisms of current clinically important SBLi. In addition to insights into the mechanisms of transient SBL inhibition by penam sulfones, the results reveal potential for penam sulfone optimization to enable irreversible SBL inhibition.
-
-	Studies on enmetazobactam clarify mechanisms of widely used beta-lactamase inhibitors.,Lang PA, Raj R, Tumber A, Lohans CT, Rabe P, Robinson CV, Brem J, Schofield CJ Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2117310119. doi:, 10.1073/pnas.2117310119. Epub 2022 Apr 29. PMID:35486701<ref>PMID:35486701</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7b3u" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brem, J]]	+	[[Category: Brem J]]
-	[[Category: Lang~~, P A~~]]	+	[[Category: Lang PA]]
-	[[Category: Schofield~~, C J]]~~	+	[[Category: Schofield CJ]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Beta lactamase]]~~	+
-	~~[[Category: Covalent modification]]~~	+
-	~~[[Category: Dehydroalanine]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Irreversible inhibition]]~~	+
-	~~[[Category: Tazobactam~~]]	+

OCA at 06:25, 12 May 2022

OCA — Thu, 12 May 2022 06:25:12 GMT

←Older revision		Revision as of 06:25, 12 May 2022
Line 1:		Line 1:

	==OXA-10 beta-lactamase with covalent modification==		==OXA-10 beta-lactamase with covalent modification==
-	<StructureSection load='7b3u' size='340' side='right'caption='[[7b3u]]' scene=''>	+	<StructureSection load='7b3u' size='340' side='right'caption='[[7b3u]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7b3u]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3u OCA], [https://pdbe.org/7b3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3u RCSB], [https://www.ebi.ac.uk/pdbsum/7b3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3u ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWW:'>SWW</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3u OCA], [https://pdbe.org/7b3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3u RCSB], [https://www.ebi.ac.uk/pdbsum/7b3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI]] Hydrolyzes both carbenicillin and oxacillin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SignificanceMicrobial resistance to beta-lactam antibiotics mediated by beta-lactamase-catalyzed hydrolysis is a major global health concern. Penam sulfones, which are structurally related to penicillins, inhibit clinically important serine beta-lactamases (SBLs) by forming transiently stable covalent complexes, thereby protecting beta-lactam antibiotics from hydrolysis. The characterization of these complexes and mechanisms of SBL inhibition is important for development of new SBL inhibitors (SBLi). Studies on the mechanism of the new SBLi enmetazobactam employing mass spectrometry and X-ray crystallography inform on its mode of action and also lead to reevaluation of mechanisms of current clinically important SBLi. In addition to insights into the mechanisms of transient SBL inhibition by penam sulfones, the results reveal potential for penam sulfone optimization to enable irreversible SBL inhibition.
		+
		+	Studies on enmetazobactam clarify mechanisms of widely used beta-lactamase inhibitors.,Lang PA, Raj R, Tumber A, Lohans CT, Rabe P, Robinson CV, Brem J, Schofield CJ Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2117310119. doi:, 10.1073/pnas.2117310119. Epub 2022 Apr 29. PMID:35486701<ref>PMID:35486701</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7b3u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brem J]]	+	[[Category: Brem, J]]
-	[[Category: Lang PA]]	+	[[Category: Lang, P A]]
-	[[Category: Schofield CJ]]	+	[[Category: Schofield, C J]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Beta lactamase]]
		+	[[Category: Covalent modification]]
		+	[[Category: Dehydroalanine]]
		+	[[Category: Hydrolase]]
		+	[[Category: Irreversible inhibition]]
		+	[[Category: Tazobactam]]

OCA at 09:53, 12 January 2022

OCA — Wed, 12 Jan 2022 09:53:16 GMT

←Older revision		Revision as of 09:53, 12 January 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7b3u is ~~ON HOLD until Dec 01 2022~~	+	==OXA-10 beta-lactamase with covalent modification==
-		+	<StructureSection load='7b3u' size='340' side='right'caption='[[7b3u]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B3U FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b3u OCA], [https://pdbe.org/7b3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b3u RCSB], [https://www.ebi.ac.uk/pdbsum/7b3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b3u ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Brem J]]
		+	[[Category: Lang PA]]
		+	[[Category: Schofield CJ]]

OCA: Protected "7b3u" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2020 05:02:11 GMT

Protected "7b3u" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:02, 9 December 2020

OCA: New page: '''Unreleased structure''' The entry 7b3u is ON HOLD until Dec 01 2022 Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Dec 2020 05:02:09 GMT

New page: '''Unreleased structure''' The entry 7b3u is ON HOLD until Dec 01 2022 Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7b3u is ON HOLD until Dec 01 2022

Authors:

Description:
[[Category: Unreleased Structures]]