7b5j - Revision history

OCA at 06:29, 25 August 2021

OCA — Wed, 25 Aug 2021 06:29:27 GMT

←Older revision		Revision as of 06:29, 25 August 2021
Line 28:		Line 28:
	[[Category: Usher, B]]		[[Category: Usher, B]]
	[[Category: Anti-crispr]]		[[Category: Anti-crispr]]
		+	[[Category: Dna binding protein]]
	[[Category: Dna-binding]]		[[Category: Dna-binding]]
	[[Category: Regulator]]		[[Category: Regulator]]
	[[Category: Rna binding protein]]		[[Category: Rna binding protein]]
	[[Category: Rna-binding]]		[[Category: Rna-binding]]

OCA at 03:47, 2 July 2021

OCA — Fri, 02 Jul 2021 03:47:55 GMT

←Older revision		Revision as of 03:47, 2 July 2021
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	==Anti-CRISPR associated (Aca) protein, Aca2==		==Anti-CRISPR associated (Aca) protein, Aca2==
-	<StructureSection load='7b5j' size='340' side='right'caption='[[7b5j]]' scene=''>	+	<StructureSection load='7b5j' size='340' side='right'caption='[[7b5j]], [[Resolution\|resolution]] 1.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B5J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7b5j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectobacterium_phage_zf40 Pectobacterium phage zf40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B5J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b5j OCA], [https://pdbe.org/7b5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b5j RCSB], [https://www.ebi.ac.uk/pdbsum/7b5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b5j ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZF40_0030, aca2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1127516 Pectobacterium phage ZF40])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b5j OCA], [https://pdbe.org/7b5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b5j RCSB], [https://www.ebi.ac.uk/pdbsum/7b5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b5j ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria use adaptive CRISPR-Cas immune mechanisms to protect from invasion by bacteriophages and other mobile genetic elements. In response, bacteriophages and mobile genetic elements have co-evolved anti-CRISPR proteins to inhibit the bacterial defense. We and others have previously shown that anti-CRISPR associated (Aca) proteins can regulate this anti-CRISPR counter-attack. Here, we report the first structure of an Aca protein, the Aca2 DNA-binding transcriptional autorepressor from Pectobacterium carotovorum bacteriophage ZF40, determined to 1.34 A. Aca2 presents a conserved N-terminal helix-turn-helix DNA-binding domain and a previously uncharacterized C-terminal dimerization domain. Dimerization positions the Aca2 recognition helices for insertion into the major grooves of target DNA, supporting its role in regulating anti-CRISPRs. Furthermore, database comparisons identified uncharacterized Aca2 structural homologs in pathogenic bacteria, suggesting that Aca2 represents the first characterized member of a more widespread family of transcriptional regulators.
		+
		+	Crystal structure of the anti-CRISPR repressor Aca2.,Usher B, Birkholz N, Beck IN, Fagerlund RD, Jackson SA, Fineran PC, Blower TR J Struct Biol. 2021 Jun 8;213(3):107752. doi: 10.1016/j.jsb.2021.107752. PMID:34116143<ref>PMID:34116143</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7b5j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Birkholz N]]	+	[[Category: Pectobacterium phage zf40]]
-	[[Category: Blower TR]]	+	[[Category: Birkholz, N]]
-	[[Category: Fineran PC]]	+	[[Category: Blower, T R]]
-	[[Category: Usher B]]	+	[[Category: Fineran, P C]]
		+	[[Category: Usher, B]]
		+	[[Category: Anti-crispr]]
		+	[[Category: Dna-binding]]
		+	[[Category: Regulator]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Rna-binding]]

OCA at 07:29, 25 June 2021

OCA — Fri, 25 Jun 2021 07:29:26 GMT

←Older revision		Revision as of 07:29, 25 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7b5j is ~~ON HOLD until Paper Publication~~	+	==Anti-CRISPR associated (Aca) protein, Aca2==
-		+	<StructureSection load='7b5j' size='340' side='right'caption='[[7b5j]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B5J FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b5j OCA], [https://pdbe.org/7b5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b5j RCSB], [https://www.ebi.ac.uk/pdbsum/7b5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b5j ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Birkholz N]]
		+	[[Category: Blower TR]]
		+	[[Category: Fineran PC]]
		+	[[Category: Usher B]]

OCA at 07:35, 24 December 2020

OCA — Thu, 24 Dec 2020 07:35:32 GMT

←Older revision		Revision as of 07:35, 24 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7b5j is ON HOLD until ~~sometime in the future~~	+	The entry 7b5j is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 10:58, 16 December 2020

OCA — Wed, 16 Dec 2020 10:58:09 GMT

←Older revision		Revision as of 10:58, 16 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7b5j is ON HOLD	+	The entry 7b5j is ON HOLD until sometime in the future

	Authors:		Authors:

OCA: Protected "7b5j" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2020 05:04:53 GMT

Protected "7b5j" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:04, 9 December 2020

OCA: New page: '''Unreleased structure''' The entry 7b5j is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Dec 2020 05:04:52 GMT

New page: '''Unreleased structure''' The entry 7b5j is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7b5j is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]