7b7o - Revision history

OCA at 11:10, 14 June 2023

OCA — Wed, 14 Jun 2023 11:10:54 GMT

←Older revision		Revision as of 11:10, 14 June 2023
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	<StructureSection load='7b7o' size='340' side='right'caption='[[7b7o]]' scene=''>		<StructureSection load='7b7o' size='340' side='right'caption='[[7b7o]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B7O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7B7O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7b7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B7O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7b7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b7o OCA], [~~http~~://pdbe.org/7b7o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7b7o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7b7o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7b7o ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b7o OCA], [https://pdbe.org/7b7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b7o RCSB], [https://www.ebi.ac.uk/pdbsum/7b7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b7o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TATA_ARATH TATA_ARATH] Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across the thylakoid membrane. Involved in delta pH-dependent protein transport required for chloroplast development, especially thylakoid membrane formation. TATC and TATB mediate precursor recognition, whereas TATA facilitates translocation (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The twin-arginine translocase (Tat) transports folded proteins across the cytoplasmic membrane of prokaryotes and the thylakoid membrane of plant chloroplasts. In Gram-negative bacteria and chloroplasts, the translocon consists of three subunits, TatA, TatB, and TatC, of which TatA is responsible for the actual membrane translocation of the substrate. Herein we report on the structure, dynamics, and lipid interactions of a fully functional C-terminally truncated 'core TatA' from Arabidopsis thaliana using solution-state NMR. Our results show that TatA consists of a short N-terminal transmembrane helix (TMH), a short connecting linker (hinge) and a long region with propensity to form an amphiphilic helix (APH). The dynamics of TatA were characterized using (15) N relaxation NMR in combination with model-free analysis. The TMH has order parameters characteristic of a well-structured helix, the hinge is somewhat less rigid, while the APH has lower order parameters indicating structural flexibility. The TMH is short with a surprisingly low protection from solvent, and only the first part of the APH is protected to some extent. In order to uncover possible differences in TatA's structure and dynamics in detergent compared to in a lipid bilayer, fast-tumbling bicelles and large unilamellar vesicles were used. Results indicate that the helicity of TatA increases in both the TMH and APH in the presence of lipids, and that the N-terminal part of the TMH is significantly more rigid. The results indicate that plant TatA has a significant structural plasticity and a capability to adapt to local environments.
		+
		+	Structure and dynamics of plant TatA in micelles and lipid bilayers studied by solution NMR.,Pettersson P, Ye W, Jakob M, Tannert F, Klosgen RB, Maler L FEBS J. 2018 May;285(10):1886-1906. doi: 10.1111/febs.14452. Epub 2018 Apr 19. PMID:29654717<ref>PMID:29654717</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7b7o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jakob M]]		[[Category: Jakob M]]

OCA at 05:54, 20 January 2021

OCA — Wed, 20 Jan 2021 05:54:04 GMT

←Older revision		Revision as of 05:54, 20 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7b7o ~~is ON HOLD~~	+	==Solution structure of A. thaliana core TatA in DHPC micelles==
-		+	<StructureSection load='7b7o' size='340' side='right'caption='[[7b7o]]' scene=''>
-	~~Authors~~: ~~Pettersson, P~~., ~~Ye, W~~., ~~Jakob, M~~., ~~Tannert, F~~., ~~Klosgen, R~~.B., ~~Maler, L~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B7O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7B7O FirstGlance]. <br>
-	~~Description: Solution structure of A. thaliana core TatA in DHPC micelles~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7b7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b7o OCA], [http://pdbe.org/7b7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7b7o RCSB], [http://www.ebi.ac.uk/pdbsum/7b7o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7b7o ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Jakob, M]]	+	__TOC__
-	[[Category: ~~Tannert, F~~]]	+	</StructureSection>
-	[[Category: ~~Klosgen, R.B~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Maler, L~~]]	+	[[Category: Jakob M]]
-	[[Category: ~~Ye, W~~]]	+	[[Category: Klosgen RB]]
-	[[Category: ~~Pettersson, P~~]]	+	[[Category: Maler L]]
		+	[[Category: Pettersson P]]
		+	[[Category: Tannert F]]
		+	[[Category: Ye W]]

OCA: Protected "7b7o" [edit=sysop:move=sysop]

OCA — Wed, 16 Dec 2020 11:02:12 GMT

Protected "7b7o" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:02, 16 December 2020

OCA: New page: '''Unreleased structure''' The entry 7b7o is ON HOLD Authors: Pettersson, P., Ye, W., Jakob, M., Tannert, F., Klosgen, R.B., Maler, L. Description: Solution structure of A. thaliana co...

OCA — Wed, 16 Dec 2020 11:02:11 GMT

New page: '''Unreleased structure''' The entry 7b7o is ON HOLD Authors: Pettersson, P., Ye, W., Jakob, M., Tannert, F., Klosgen, R.B., Maler, L. Description: Solution structure of A. thaliana co...

New page

'''Unreleased structure'''

The entry 7b7o is ON HOLD

Authors: Pettersson, P., Ye, W., Jakob, M., Tannert, F., Klosgen, R.B., Maler, L.

Description: Solution structure of A. thaliana core TatA in DHPC micelles
[[Category: Unreleased Structures]]
[[Category: Jakob, M]]
[[Category: Tannert, F]]
[[Category: Klosgen, R.B]]
[[Category: Maler, L]]
[[Category: Ye, W]]
[[Category: Pettersson, P]]