7bcu - Revision history

OCA at 12:25, 1 February 2024

OCA — Thu, 01 Feb 2024 12:25:53 GMT

←Older revision		Revision as of 12:25, 1 February 2024
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	==The adduct of NAMI-A with Hen Egg White Lysozyme at 1.5 hours.==		==The adduct of NAMI-A with Hen Egg White Lysozyme at 1.5 hours.==
-	<StructureSection load='7bcu' size='340' side='right'caption='[[7bcu]]' scene=''>	+	<StructureSection load='7bcu' size='340' side='right'caption='[[7bcu]], [[Resolution\|resolution]] 0.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BCU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bcu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BCU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bcu OCA], [https://pdbe.org/7bcu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bcu RCSB], [https://www.ebi.ac.uk/pdbsum/7bcu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bcu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.98Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=RU:RUTHENIUM+ION'>RU</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bcu OCA], [https://pdbe.org/7bcu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bcu RCSB], [https://www.ebi.ac.uk/pdbsum/7bcu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bcu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The pharmacological profile of medicinally relevant Ru(III) coordination compounds has been ascribed to their interactions with proteins, as several studies have provided evidence that DNA is not the primary target. In this regard, numerous spectroscopic and crystallographic studies have indicated that the Ru(III) ligands play an important role in determining the metal binding site, acting as the recognition element in the early stages of the protein-complex formation. Herein, we present a series of near-atomic-resolution X-ray crystal structures of the adducts formed between the antimetastatic metallodrug imidazolium trans-[tetrachlorido(S-dimethyl sufoxide)(1H-imidazole)ruthenate(III)] (NAMI-A) and hen egg-white lysozyme (HEWL). These structures elucidate a series of binding events starting from the noncovalent interaction of intact NAMI-A ions with HEWL (1.5 h), followed by the stepwise exchange of all Ru ligands except for 1H-imidazole (26 h) to the final "ruthenated" protein comprising one aquated Ru ion coordinated to histidine-15 of HEWL (98 h). Our structural data clearly support a two-step mechanism of protein ruthenation, illustrating the ligand-mediated recognition step of the process.
		+
		+	Insights into the Protein Ruthenation Mechanism by Antimetastatic Metallodrugs: High-Resolution X-ray Structures of the Adduct Formed between Hen Egg-White Lysozyme and NAMI-A at Various Time Points.,Chiniadis L, Giastas P, Bratsos I, Papakyriakou A Inorg Chem. 2021 Jul 19;60(14):10729-10737. doi: 10.1021/acs.inorgchem.1c01441., Epub 2021 Jul 1. PMID:34197115<ref>PMID:34197115</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bcu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bratsos I]]		[[Category: Bratsos I]]

OCA at 10:31, 28 July 2021

OCA — Wed, 28 Jul 2021 10:31:58 GMT

←Older revision		Revision as of 10:31, 28 July 2021
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-	'''Unreleased structure'''

-	The ~~entry~~ 7bcu is ~~ON HOLD until Paper Publication~~	+	==The adduct of NAMI-A with Hen Egg White Lysozyme at 1.5 hours.==
-		+	<StructureSection load='7bcu' size='340' side='right'caption='[[7bcu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BCU FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bcu OCA], [https://pdbe.org/7bcu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bcu RCSB], [https://www.ebi.ac.uk/pdbsum/7bcu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bcu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bratsos I]]
		+	[[Category: Chiniadis L]]
		+	[[Category: Giastas P]]
		+	[[Category: Papakyriakou A]]

OCA at 08:36, 14 July 2021

OCA — Wed, 14 Jul 2021 08:36:53 GMT

←Older revision		Revision as of 08:36, 14 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bcu is ON HOLD	+	The entry 7bcu is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 06:00, 25 June 2021

OCA — Fri, 25 Jun 2021 06:00:18 GMT

←Older revision		Revision as of 06:00, 25 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bcu is ON HOLD ~~until Paper Publication~~	+	The entry 7bcu is ON HOLD

	Authors:		Authors:

OCA at 06:36, 20 January 2021

OCA — Wed, 20 Jan 2021 06:36:12 GMT

←Older revision		Revision as of 06:36, 20 January 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bcu is ON HOLD	+	The entry 7bcu is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7bcu" [edit=sysop:move=sysop]

OCA — Wed, 30 Dec 2020 05:16:43 GMT

Protected "7bcu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 30 December 2020

OCA: New page: '''Unreleased structure''' The entry 7bcu is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 Dec 2020 05:16:42 GMT

New page: '''Unreleased structure''' The entry 7bcu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bcu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]