OCA at 12:26, 1 February 2024

OCA — Thu, 01 Feb 2024 12:26:32 GMT

←Older revision		Revision as of 12:26, 1 February 2024
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-	====	+	==14-3-3 sigma with Pin1 binding site pS72 and covalently bound PC2068B==
-	<StructureSection load='7bdy' size='340' side='right'caption='[[7bdy]]' scene=''>	+	<StructureSection load='7bdy' size='340' side='right'caption='[[7bdy]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bdy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BDY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BDY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bdy OCA], [https://pdbe.org/7bdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bdy RCSB], [https://www.ebi.ac.uk/pdbsum/7bdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bdy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.801Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TJK:2-bromanyl-4-[2-(5-bromanyl-2-fluoranyl-phenyl)imidazol-1-yl]benzaldehyde'>TJK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bdy OCA], [https://pdbe.org/7bdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bdy RCSB], [https://www.ebi.ac.uk/pdbsum/7bdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bdy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The stabilization of protein complexes has emerged as a promising modality, expanding the number of entry points for novel therapeutic intervention. Targeting proteins that mediate protein-protein interactions (PPIs), such as hub proteins, is equally challenging and rewarding as they offer an intervention platform for a variety of diseases, due to their large interactome. 14-3-3 hub proteins bind phosphorylated motifs of their interaction partners in a conserved binding channel. The 14-3-3 PPI interface is consequently only diversified by its different interaction partners. Therefore, it is essential to consider, additionally to the potency, also the selectivity of stabilizer molecules. Targeting a lysine residue at the interface of the composite 14-3-3 complex, which can be targeted explicitly via aldimine-forming fragments, we studied the de novo design of PPI stabilizers under consideration of potential selectivity. By applying cooperativity analysis of ternary complex formation, we developed a reversible covalent molecular glue for the 14-3-3/Pin1 interaction. This small fragment led to a more than 250-fold stabilization of the 14-3-3/Pin1 interaction by selective interfacing with a unique tryptophan in Pin1. This study illustrates how cooperative complex formation drives selective PPI stabilization. Further, it highlights how specific interactions within a hub proteins interactome can be stabilized over other interactions with a common binding motif.
		+
		+	Reversible Covalent Imine-Tethering for Selective Stabilization of 14-3-3 Hub Protein Interactions.,Cossar PJ, Wolter M, van Dijck L, Valenti D, Levy LM, Ottmann C, Brunsveld L J Am Chem Soc. 2021 Jun 9;143(22):8454-8464. doi: 10.1021/jacs.1c03035. Epub 2021 , May 28. PMID:34047554<ref>PMID:34047554</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bdy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Cossar PJ]]
		+	[[Category: Dijck Lv]]
		+	[[Category: Ottmann C]]
		+	[[Category: Wolter M]]

OCA at 14:45, 17 June 2021

OCA — Thu, 17 Jun 2021 14:45:27 GMT

←Older revision		Revision as of 14:45, 17 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7bdy ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7bdy' size='340' side='right'caption='[[7bdy]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bdy OCA], [https://pdbe.org/7bdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bdy RCSB], [https://www.ebi.ac.uk/pdbsum/7bdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bdy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7bdy" [edit=sysop:move=sysop]

OCA — Wed, 30 Dec 2020 05:18:39 GMT

Protected "7bdy" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 30 December 2020

OCA: New page: '''Unreleased structure''' The entry 7bdy is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 Dec 2020 05:18:38 GMT

New page: '''Unreleased structure''' The entry 7bdy is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bdy is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7bdy - Revision history

OCA at 12:26, 1 February 2024

OCA at 14:45, 17 June 2021

OCA: Protected "7bdy" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7bdy is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures