7bga - Revision history

OCA at 12:28, 1 February 2024

OCA — Thu, 01 Feb 2024 12:28:00 GMT

←Older revision		Revision as of 12:28, 1 February 2024
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	<StructureSection load='7bga' size='340' side='right'caption='[[7bga]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='7bga' size='340' side='right'caption='[[7bga]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7bga]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BGA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bga]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BGA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LGB ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9913 BOVIN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bga OCA], [https://pdbe.org/7bga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bga RCSB], [https://www.ebi.ac.uk/pdbsum/7bga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bga ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bga OCA], [https://pdbe.org/7bga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bga RCSB], [https://www.ebi.ac.uk/pdbsum/7bga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bga ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.	+	[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7bga" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7bga" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactoglobulin 3D structures\|Beta-lactoglobulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lazinska, I]]	+	[[Category: Lazinska I]]
-	[[Category: Lewinski, K]]	+	[[Category: Lewinski K]]
-	[[Category: Loch~~, J I]]~~	+	[[Category: Loch JI]]
-	~~[[Category: Lactoglobulin]]~~	+
-	~~[[Category: Ligand]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 10:47, 31 March 2021

OCA — Wed, 31 Mar 2021 10:47:03 GMT

←Older revision		Revision as of 10:47, 31 March 2021
Line 1:		Line 1:

	==Mutant F105A of recombinant beta-lactoglobulin in complex with endogenous ligand==		==Mutant F105A of recombinant beta-lactoglobulin in complex with endogenous ligand==
-	<StructureSection load='7bga' size='340' side='right'caption='[[7bga]]' scene=''>	+	<StructureSection load='7bga' size='340' side='right'caption='[[7bga]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BGA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7BGA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bga]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BGA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7bga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bga OCA], [~~http~~://pdbe.org/7bga PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7bga RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7bga PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7bga ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bga OCA], [https://pdbe.org/7bga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bga RCSB], [https://www.ebi.ac.uk/pdbsum/7bga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bga ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-Lactoglobulin (BLG) like other lipocalins can be modified by mutagenesis to re-direct its ligand binding properties. Local site-directed mutagenesis was used to change the geometry of the BLG ligand binding pocket and therefore change BLG ligand preferences. The presented studies are focused on previously described mutants L39Y, I56F, L58F, F105L, and M107L and two new BLG variants, L39K and F105A, and their interactions with local anesthetic drug tetracaine. Binding of tetracaine to BLG mutants was investigated by X-ray crystallography. Structural analysis revealed that for tetracaine binding, the shape of the binding pocket seems to be a more important factor than the substitutions influencing the number of interactions. Analyzed BLG mutants can be classified according to their binding properties to variants: capable of binding tetracaine in the beta-barrel (L58F, M107L); capable of accommodating tetracaine on the protein surface (I56F) and unable to bind tetracaine (F105L). Variants L39K, L39Y, and F105A, had a binding pocket blocked by endogenous fatty acids. The new tetracaine binding site was found in the I56F variant. The site localized on the surface near Arg124 and Trp19 was previously predicted by in silico studies and was confirmed in the crystal structure.
		+
		+	Interactions of new lactoglobulin variants with tetracaine: crystallographic studies of ligand binding to lactoglobulin mutants possessing single substitution in the binding pocket.,Loch J, Bonarek P, Siuda M, Wrobel P, Lewinski K Acta Biochim Pol. 2021 Mar 15;68(1):23-28. doi: 10.18388/abp.2020_5593. PMID:33719368<ref>PMID:33719368</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bga" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lazinska I]]	+	[[Category: Lazinska, I]]
-	[[Category: Lewinski K]]	+	[[Category: Lewinski, K]]
-	[[Category: Loch JI]]	+	[[Category: Loch, J I]]
		+	[[Category: Lactoglobulin]]
		+	[[Category: Ligand]]
		+	[[Category: Mutant]]
		+	[[Category: Transport protein]]

OCA: Protected "7bga" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2021 08:13:24 GMT

Protected "7bga" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:13, 20 January 2021

OCA: New page: ==Mutant F105A of recombinant beta-lactoglobulin in complex with endogenous ligand== == Structural highli...

OCA — Wed, 20 Jan 2021 08:13:23 GMT

New page: ==Mutant F105A of recombinant beta-lactoglobulin in complex with endogenous ligand== <StructureSection load='7bga' size='340' side='right'caption='7bga' scene=''> == Structural highli...

New page

==Mutant F105A of recombinant beta-lactoglobulin in complex with endogenous ligand==
<StructureSection load='7bga' size='340' side='right'caption='[[7bga]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BGA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BGA FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bga OCA], [http://pdbe.org/7bga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bga RCSB], [http://www.ebi.ac.uk/pdbsum/7bga PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bga ProSAT]</span></td></tr>
</table>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lazinska I]]
[[Category: Lewinski K]]
[[Category: Loch JI]]