7bh0 - Revision history

OCA at 12:28, 1 February 2024

OCA — Thu, 01 Feb 2024 12:28:18 GMT

←Older revision		Revision as of 12:28, 1 February 2024
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	<StructureSection load='7bh0' size='340' side='right'caption='[[7bh0]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='7bh0' size='340' side='right'caption='[[7bh0]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7bh0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BH0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bh0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BH0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LGB ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9913 BOVIN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bh0 OCA], [https://pdbe.org/7bh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bh0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bh0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bh0 OCA], [https://pdbe.org/7bh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bh0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bh0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.	+	[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7bh0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7bh0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactoglobulin 3D structures\|Beta-lactoglobulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lewinski, K]]	+	[[Category: Lewinski K]]
-	[[Category: Loch~~, J I~~]]	+	[[Category: Loch JI]]
-	[[Category: Siuda~~, M K]]~~	+	[[Category: Siuda MK]]
-	~~[[Category: Beta-lactoglobulin]]~~	+
-	~~[[Category: Lipocalin]]~~	+
-	~~[[Category: Mutation]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 10:47, 31 March 2021

OCA — Wed, 31 Mar 2021 10:47:08 GMT

←Older revision		Revision as of 10:47, 31 March 2021
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	==Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenous ligand==		==Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenous ligand==
-	<StructureSection load='7bh0' size='340' side='right'caption='[[7bh0]]' scene=''>	+	<StructureSection load='7bh0' size='340' side='right'caption='[[7bh0]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BH0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bh0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BH0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bh0 OCA], [https://pdbe.org/7bh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bh0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bh0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bh0 OCA], [https://pdbe.org/7bh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bh0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bh0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-Lactoglobulin (BLG) like other lipocalins can be modified by mutagenesis to re-direct its ligand binding properties. Local site-directed mutagenesis was used to change the geometry of the BLG ligand binding pocket and therefore change BLG ligand preferences. The presented studies are focused on previously described mutants L39Y, I56F, L58F, F105L, and M107L and two new BLG variants, L39K and F105A, and their interactions with local anesthetic drug tetracaine. Binding of tetracaine to BLG mutants was investigated by X-ray crystallography. Structural analysis revealed that for tetracaine binding, the shape of the binding pocket seems to be a more important factor than the substitutions influencing the number of interactions. Analyzed BLG mutants can be classified according to their binding properties to variants: capable of binding tetracaine in the beta-barrel (L58F, M107L); capable of accommodating tetracaine on the protein surface (I56F) and unable to bind tetracaine (F105L). Variants L39K, L39Y, and F105A, had a binding pocket blocked by endogenous fatty acids. The new tetracaine binding site was found in the I56F variant. The site localized on the surface near Arg124 and Trp19 was previously predicted by in silico studies and was confirmed in the crystal structure.
		+
		+	Interactions of new lactoglobulin variants with tetracaine: crystallographic studies of ligand binding to lactoglobulin mutants possessing single substitution in the binding pocket.,Loch J, Bonarek P, Siuda M, Wrobel P, Lewinski K Acta Biochim Pol. 2021 Mar 15;68(1):23-28. doi: 10.18388/abp.2020_5593. PMID:33719368<ref>PMID:33719368</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bh0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lewinski K]]	+	[[Category: Lewinski, K]]
-	[[Category: Loch JI]]	+	[[Category: Loch, J I]]
-	[[Category: Siuda MK]]	+	[[Category: Siuda, M K]]
		+	[[Category: Beta-lactoglobulin]]
		+	[[Category: Lipocalin]]
		+	[[Category: Mutation]]
		+	[[Category: Transport protein]]

OCA at 07:56, 27 January 2021

OCA — Wed, 27 Jan 2021 07:56:36 GMT

←Older revision		Revision as of 07:56, 27 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7bh0 ~~is ON HOLD~~	+	==Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenous ligand==
-		+	<StructureSection load='7bh0' size='340' side='right'caption='[[7bh0]]' scene=''>
-	~~Authors~~: ~~Loch~~, J.I., ~~Siuda~~, M.K., ~~Lewinski~~, K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BH0 FirstGlance]. <br>
-	~~Description: Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenous ligand~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bh0 OCA], [https://pdbe.org/7bh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bh0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bh0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Loch, J.I~~]]	+	__TOC__
-	[[Category: ~~Lewinski, K~~]]	+	</StructureSection>
-	[[Category: Siuda~~, M.K~~]]	+	[[Category: Large Structures]]
		+	[[Category: Lewinski K]]
		+	[[Category: Loch JI]]
		+	[[Category: Siuda MK]]

OCA: Protected "7bh0" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2021 06:39:27 GMT

Protected "7bh0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:39, 20 January 2021

OCA: New page: '''Unreleased structure''' The entry 7bh0 is ON HOLD Authors: Loch, J.I., Siuda, M.K., Lewinski, K. Description: Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenou...

OCA — Wed, 20 Jan 2021 06:39:26 GMT

New page: '''Unreleased structure''' The entry 7bh0 is ON HOLD Authors: Loch, J.I., Siuda, M.K., Lewinski, K. Description: Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenou...

New page

'''Unreleased structure'''

The entry 7bh0 is ON HOLD

Authors: Loch, J.I., Siuda, M.K., Lewinski, K.

Description: Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenous ligand
[[Category: Unreleased Structures]]
[[Category: Loch, J.I]]
[[Category: Lewinski, K]]
[[Category: Siuda, M.K]]