7bix - Revision history

OCA at 12:29, 1 February 2024

OCA — Thu, 01 Feb 2024 12:29:12 GMT

←Older revision		Revision as of 12:29, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7bix]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[7bix]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UTP:URIDINE+5-TRIPHOSPHATE'>UTP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.12Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UTP:URIDINE+5-TRIPHOSPHATE'>UTP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bix OCA], [https://pdbe.org/7bix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bix RCSB], [https://www.ebi.ac.uk/pdbsum/7bix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bix ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bix OCA], [https://pdbe.org/7bix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bix RCSB], [https://www.ebi.ac.uk/pdbsum/7bix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PYRH_MYCTU PYRH_MYCTU]] Catalyzes the reversible phosphorylation of UMP to UDP.[HAMAP-Rule:MF_01220]	+	[https://www.uniprot.org/uniprot/PYRH_MYCTU PYRH_MYCTU] Catalyzes the reversible phosphorylation of UMP to UDP.[HAMAP-Rule:MF_01220]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:00, 14 September 2022

OCA — Wed, 14 Sep 2022 07:00:36 GMT

←Older revision		Revision as of 07:00, 14 September 2022
Line 1:		Line 1:

	==Crystal structure of UMPK from M. tuberculosis in complex with UDP and UTP (C2 form)==		==Crystal structure of UMPK from M. tuberculosis in complex with UDP and UTP (C2 form)==
-	<StructureSection load='7bix' size='340' side='right'caption='[[7bix]]' scene=''>	+	<StructureSection load='7bix' size='340' side='right'caption='[[7bix]], [[Resolution\|resolution]] 3.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bix]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bix OCA], [https://pdbe.org/7bix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bix RCSB], [https://www.ebi.ac.uk/pdbsum/7bix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bix ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UTP:URIDINE+5-TRIPHOSPHATE'>UTP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bix OCA], [https://pdbe.org/7bix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bix RCSB], [https://www.ebi.ac.uk/pdbsum/7bix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bix ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/PYRH_MYCTU PYRH_MYCTU]] Catalyzes the reversible phosphorylation of UMP to UDP.[HAMAP-Rule:MF_01220]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tuberculosis claims significantly more than one million lives each year. A feasible way to face the issue of drug resistance is the development of new antibiotics. Bacterial uridine 5'-monophosphate (UMP) kinase is a promising target for novel antibiotic discovery as it is essential for bacterial survival and has no counterpart in human cells. The UMP kinase from M. tuberculosis is also a model of particular interest for allosteric regulation with two effectors, GTP (positive) and UTP (negative). In this study, using X-ray crystallography and cryo-electron microscopy, we report for the first time a detailed description of the negative effector UTP-binding site of a typical Gram-positive behaving UMP kinase. Comparison between this snapshot of low affinity for Mg-ATP with our previous 3D-structure of the GTP-bound complex of high affinity for Mg-ATP led to a better understanding of the cooperative mechanism and the allosteric regulation of UMP kinase. Thermal shift assay and circular dichroism experiments corroborate our model of an inhibition by UTP linked to higher flexibility of the Mg-ATP-binding domain. These new structural insights provide valuable knowledge for future drug discovery strategies targeting bacterial UMP kinases.
		+
		+	Structural basis for the allosteric inhibition of UMP kinase from Gram-positive bacteria, a promising antibacterial target.,Walter P, Mechaly A, Bous J, Haouz A, England P, Lai-Kee-Him J, Ancelin A, Hoos S, Baron B, Trapani S, Bron P, Labesse G, Munier-Lehmann H FEBS J. 2022 Feb 12. doi: 10.1111/febs.16393. PMID:35152545<ref>PMID:35152545</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bix" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Uridylate kinase\|Uridylate kinase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mycobacterium tuberculosis H37Rv]]
	[[Category: Haouz A]]		[[Category: Haouz A]]
	[[Category: Labesse G]]		[[Category: Labesse G]]

OCA at 06:14, 2 March 2022

OCA — Wed, 02 Mar 2022 06:14:46 GMT

←Older revision		Revision as of 06:14, 2 March 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7bix is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of UMPK from M. tuberculosis in complex with UDP and UTP (C2 form)==
-		+	<StructureSection load='7bix' size='340' side='right'caption='[[7bix]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIX FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bix OCA], [https://pdbe.org/7bix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bix RCSB], [https://www.ebi.ac.uk/pdbsum/7bix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bix ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Haouz A]]
		+	[[Category: Labesse G]]
		+	[[Category: Mechaly AE]]
		+	[[Category: Munier-Lehmann H]]
		+	[[Category: Walter P]]

OCA at 08:33, 17 February 2021

OCA — Wed, 17 Feb 2021 08:33:22 GMT

←Older revision		Revision as of 08:33, 17 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bix is ON HOLD	+	The entry 7bix is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7bix" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2021 06:42:48 GMT

Protected "7bix" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:42, 20 January 2021

OCA: New page: '''Unreleased structure''' The entry 7bix is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Jan 2021 06:42:47 GMT

New page: '''Unreleased structure''' The entry 7bix is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bix is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]