7bk0 - Revision history

OCA at 07:48, 1 May 2024

OCA — Wed, 01 May 2024 07:48:41 GMT

←Older revision		Revision as of 07:48, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='7bk0' size='340' side='right'caption='[[7bk0]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='7bk0' size='340' side='right'caption='[[7bk0]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7bk0]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp.~~_enterica_serovar_typhimurium~~ Salmonella enterica subsp. enterica serovar ~~typhimurium~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bk0]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK0 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[7bj2\|7bj2]]~~, [[~~7bgl~~\|~~7bgl~~]]~~, [[7bhq\|7bhq]], [[7bin\|7bin]]</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk0 OCA], [https://pdbe.org/7bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk0 OCA], [https://pdbe.org/7bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY]] The M ring may be actively involved in energy transduction.	+	[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY] The M ring may be actively involved in energy transduction.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids(1-3). Here, we present structures of the intact Salmonella flagellar basal body(4), encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 A. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine.	+
-		+
-	Molecular structure of the intact bacterial flagellar basal body.,Johnson S, Furlong EJ, Deme JC, Nord AL, Caesar JJE, Chevance FFV, Berry RM, Hughes KT, Lea SM Nat Microbiol. 2021 Apr 30. pii: 10.1038/s41564-021-00895-y. doi:, 10.1038/s41564-021-00895-y. PMID:33931760<ref>PMID:33931760</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7bk0" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Salmonella enterica subsp. enterica serovar ~~typhimurium~~]]	+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-	[[Category: Furlong, E]]	+	[[Category: Furlong E]]
-	[[Category: Johnson, S]]	+	[[Category: Johnson S]]
-	[[Category: Lea~~, S M]]~~	+	[[Category: Lea SM]]
-	~~[[Category: Bacterial flagellum basal body ms-ring]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 10:23, 19 May 2021

OCA — Wed, 19 May 2021 10:23:12 GMT

←Older revision		Revision as of 10:23, 19 May 2021
Line 1:		Line 1:

	==Salmonella FliF ring (34mer) in intact basal body - C1==		==Salmonella FliF ring (34mer) in intact basal body - C1==
-	<StructureSection load='7bk0' size='340' side='right'caption='[[7bk0]]' scene=''>	+	<StructureSection load='7bk0' size='340' side='right'caption='[[7bk0]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bk0]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk0 OCA], [https://pdbe.org/7bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk0 ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7bj2\|7bj2]], [[7bgl\|7bgl]], [[7bhq\|7bhq]], [[7bin\|7bin]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk0 OCA], [https://pdbe.org/7bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY]] The M ring may be actively involved in energy transduction.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids(1-3). Here, we present structures of the intact Salmonella flagellar basal body(4), encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 A. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine.
		+
		+	Molecular structure of the intact bacterial flagellar basal body.,Johnson S, Furlong EJ, Deme JC, Nord AL, Caesar JJE, Chevance FFV, Berry RM, Hughes KT, Lea SM Nat Microbiol. 2021 Apr 30. pii: 10.1038/s41564-021-00895-y. doi:, 10.1038/s41564-021-00895-y. PMID:33931760<ref>PMID:33931760</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bk0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Furlong E]]	+	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
-	[[Category: Johnson S]]	+	[[Category: Furlong, E]]
-	[[Category: Lea SM]]	+	[[Category: Johnson, S]]
		+	[[Category: Lea, S M]]
		+	[[Category: Bacterial flagellum basal body ms-ring]]
		+	[[Category: Protein transport]]

OCA at 08:52, 5 May 2021

OCA — Wed, 05 May 2021 08:52:07 GMT

←Older revision		Revision as of 08:52, 5 May 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7bk0 ~~is ON HOLD until Paper Publication~~	+	==Salmonella FliF ring (34mer) in intact basal body - C1==
-		+	<StructureSection load='7bk0' size='340' side='right'caption='[[7bk0]]' scene=''>
-	~~Authors~~: ~~Johnson~~, S., ~~Furlong~~, E., ~~Lea~~, S.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK0 FirstGlance]. <br>
-	~~Description: Salmonella FliF ring (34mer) in intact basal body -C1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk0 OCA], [https://pdbe.org/7bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Furlong, E]]	+	__TOC__
-	[[Category: Johnson, S]]	+	</StructureSection>
-	[[Category: Lea~~, S.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Furlong E]]
		+	[[Category: Johnson S]]
		+	[[Category: Lea SM]]

OCA at 05:26, 24 February 2021

OCA — Wed, 24 Feb 2021 05:26:28 GMT

←Older revision		Revision as of 05:26, 24 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bk0 is ON HOLD	+	The entry 7bk0 is ON HOLD until Paper Publication

	Authors: Johnson, S., Furlong, E., Lea, S.M.		Authors: Johnson, S., Furlong, E., Lea, S.M.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Furlong, E]]		[[Category: Furlong, E]]
-	[[Category: Lea, S.M]]
	[[Category: Johnson, S]]		[[Category: Johnson, S]]
		+	[[Category: Lea, S.M]]

OCA: Protected "7bk0" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2021 06:45:27 GMT

Protected "7bk0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:45, 20 January 2021

OCA: New page: '''Unreleased structure''' The entry 7bk0 is ON HOLD Authors: Johnson, S., Furlong, E., Lea, S.M. Description: Salmonella FliF ring (34mer) in intact basal body -C1 [[Category: Unrelea...

OCA — Wed, 20 Jan 2021 06:45:26 GMT

New page: '''Unreleased structure''' The entry 7bk0 is ON HOLD Authors: Johnson, S., Furlong, E., Lea, S.M. Description: Salmonella FliF ring (34mer) in intact basal body -C1 [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 7bk0 is ON HOLD

Authors: Johnson, S., Furlong, E., Lea, S.M.

Description: Salmonella FliF ring (34mer) in intact basal body -C1
[[Category: Unreleased Structures]]
[[Category: Furlong, E]]
[[Category: Lea, S.M]]
[[Category: Johnson, S]]