OCA at 12:29, 1 February 2024

OCA — Thu, 01 Feb 2024 12:29:58 GMT

←Older revision		Revision as of 12:29, 1 February 2024
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	==PfCopC mutant - D83N==		==PfCopC mutant - D83N==
-	<StructureSection load='7bk7' size='340' side='right'caption='[[7bk7]]' scene=''>	+	<StructureSection load='7bk7' size='340' side='right'caption='[[7bk7]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens_SBW25 Pseudomonas fluorescens SBW25]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk7 OCA], [https://pdbe.org/7bk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk7 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk7 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk7 OCA], [https://pdbe.org/7bk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk7 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C3JYL7_PSEFS C3JYL7_PSEFS] Involved in copper resistance.[RuleBase:RU369037]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The histidine brace (His-brace) is a copper-binding motif that is associated with both oxidative enzymes and proteinaceous copper chaperones. Here, we used biochemical and structural methods to characterize mutants of a His-brace-containing copper chaperone from Pseudomonas fluorescens (PfCopC). A total of 15 amino acid variants in primary and second-sphere residues were produced and characterized in terms of their copper binding and redox properties. PfCopC has a very high affinity for Cu(II) and also binds Cu(I). A high reorganization barrier likely prevents redox cycling and, thus, catalysis. In contrast, mutations in the conserved second-sphere Glu27 enable slow oxidation of ascorbate. The crystal structure of the variant E27A confirmed copper binding at the His-brace. Unexpectedly, Asp83 at the equatorial position was shown to be indispensable for Cu(II) binding in the His-brace of PfCopC. A PfCopC mutant that was designed to mimic the His-brace from lytic polysaccharide monooxygenase-like family X325 did not bind Cu(II), but was still able to bind Cu(I). These results highlight the importance of the proteinaceous environment around the copper His-brace for reactivity and, thus, the difference between enzyme and chaperone.
		+
		+	Copper binding and reactivity at the histidine brace motif: insights from mutational analysis of the Pseudomonas fluorescens copper chaperone CopC.,Ipsen JO, Hernandez-Rollan C, Muderspach SJ, Brander S, Bertelsen AB, Jensen PE, Norholm MHH, Lo Leggio L, Johansen KS FEBS Lett. 2021 Jun;595(12):1708-1720. doi: 10.1002/1873-3468.14092. Epub 2021, May 14. PMID:33896006<ref>PMID:33896006</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bk7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudomonas fluorescens SBW25]]
	[[Category: Bertelsen AB]]		[[Category: Bertelsen AB]]
	[[Category: Ipsen J]]		[[Category: Ipsen J]]

OCA at 09:55, 7 July 2021

OCA — Wed, 07 Jul 2021 09:55:36 GMT

←Older revision		Revision as of 09:55, 7 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7bk7 is ~~ON HOLD until Paper Publication~~	+	==PfCopC mutant - D83N==
-		+	<StructureSection load='7bk7' size='340' side='right'caption='[[7bk7]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK7 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk7 OCA], [https://pdbe.org/7bk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk7 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk7 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bertelsen AB]]
		+	[[Category: Ipsen J]]
		+	[[Category: Johansen KS]]
		+	[[Category: Lo Leggio L]]
		+	[[Category: Muderspach SJ]]
		+	[[Category: Norholm MHH]]
		+	[[Category: Rollan CH]]

OCA at 09:12, 3 February 2021

OCA — Wed, 03 Feb 2021 09:12:45 GMT

←Older revision		Revision as of 09:12, 3 February 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bk7 is ON HOLD	+	The entry 7bk7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7bk7" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2021 06:45:46 GMT

Protected "7bk7" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:45, 20 January 2021

OCA: New page: '''Unreleased structure''' The entry 7bk7 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Jan 2021 06:45:45 GMT

New page: '''Unreleased structure''' The entry 7bk7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bk7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7bk7 - Revision history

OCA at 12:29, 1 February 2024

OCA at 09:55, 7 July 2021

OCA at 09:12, 3 February 2021

OCA: Protected "7bk7" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7bk7 is ON HOLD Authors: Description: Category: Unreleased Structures