7bpv - Revision history

OCA at 15:24, 29 November 2023

OCA — Wed, 29 Nov 2023 15:24:51 GMT

←Older revision		Revision as of 15:24, 29 November 2023
Line 1:		Line 1:

-	====	+	==r(GUGGGCCGAC)/d(GTCGGCCCAC) hybrid duplex structure==
-	<StructureSection load='7bpv' size='340' side='right'caption='[[7bpv]]' scene=''>	+	<StructureSection load='7bpv' size='340' side='right'caption='[[7bpv]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bpv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BPV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bpv OCA], [https://pdbe.org/7bpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bpv RCSB], [https://www.ebi.ac.uk/pdbsum/7bpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bpv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.781Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bpv OCA], [https://pdbe.org/7bpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bpv RCSB], [https://www.ebi.ac.uk/pdbsum/7bpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bpv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The use of a small molecule compound to reduce toxic repeat RNA transcripts or their translated aberrant proteins to target repeat-expanded RNA/DNA with a G4C2 motif is a promising strategy to treat C9orf72-linked disorders. In this study, the crystal structures of DNA and RNA-DNA hybrid duplexes with the -GGGCCG- region as a G4C2 repeat motif were solved. Unusual groove widening and sharper bending of the G4C2 DNA duplex A-DNA conformation with B-form characteristics inside was observed. The G4C2 RNA-DNA hybrid duplex adopts a more typical rigid A form structure. Detailed structural analysis revealed that the G4C2 repeat motif of the DNA duplex exhibits a hydration shell and greater flexibility and serves as a 'hot-spot' for binding of the anthracene-based nickel complex, NiII(Chro)2 (Chro = Chromomycin A3). In addition to the original GGCC recognition site, NiII(Chro)2 has extended specificity and binds the flanked G:C base pairs of the GGCC core, resulting in minor groove contraction and straightening of the DNA backbone. We have also shown that Chro-metal complexes inhibit neuronal toxicity and suppresses locomotor deficits in a Drosophila model of C9orf72-associated ALS. The approach represents a new direction for drug discovery against ALS and FTD diseases by targeting G4C2 repeat motif DNA.
		+
		+	Targeting the ALS/FTD-associated A-DNA kink with anthracene-based metal complex causes DNA backbone straightening and groove contraction.,Jhan CR, Satange R, Wang SC, Zeng JY, Horng YC, Jin P, Neidle S, Hou MH Nucleic Acids Res. 2021 Sep 20;49(16):9526-9538. doi: 10.1093/nar/gkab227. PMID:33836081<ref>PMID:33836081</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bpv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Hou MH]]
		+	[[Category: Satange RB]]
		+	[[Category: Wang SC]]

OCA at 14:45, 3 March 2021

OCA — Wed, 03 Mar 2021 14:45:05 GMT

←Older revision		Revision as of 14:45, 3 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7bpv ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7bpv' size='340' side='right'caption='[[7bpv]]' scene=''>
-	~~Authors~~: ~~Wang, S~~.C.~~, Satange, R~~.B.~~, Hou, M~~.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~r(GUGGGCCGAC)~~/~~d(GTCGGCCCAC) hybrid duplex structure~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bpv OCA], [https://pdbe.org/7bpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bpv RCSB], [https://www.ebi.ac.uk/pdbsum/7bpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bpv ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Satange~~, R.B]]	+	__TOC__
-	[[Category: ~~Wang, S.C~~]]	+	</StructureSection>
-	[[Category: ~~Hou, M.H~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7bpv" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2020 07:53:02 GMT

Protected "7bpv" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:53, 1 April 2020

OCA: New page: '''Unreleased structure''' The entry 7bpv is ON HOLD until Paper Publication Authors: Wang, S.C., Satange, R.B., Hou, M.H. Description: r(GUGGGCCGAC)/d(GTCGGCCCAC) hybrid duplex struct...

OCA — Wed, 01 Apr 2020 07:53:00 GMT

New page: '''Unreleased structure''' The entry 7bpv is ON HOLD until Paper Publication Authors: Wang, S.C., Satange, R.B., Hou, M.H. Description: r(GUGGGCCGAC)/d(GTCGGCCCAC) hybrid duplex struct...

New page

'''Unreleased structure'''

The entry 7bpv is ON HOLD until Paper Publication

Authors: Wang, S.C., Satange, R.B., Hou, M.H.

Description: r(GUGGGCCGAC)/d(GTCGGCCCAC) hybrid duplex structure
[[Category: Unreleased Structures]]
[[Category: Satange, R.B]]
[[Category: Wang, S.C]]
[[Category: Hou, M.H]]