OCA at 06:36, 7 April 2023

OCA — Fri, 07 Apr 2023 06:36:03 GMT

←Older revision		Revision as of 06:36, 7 April 2023
Line 1:		Line 1:

-	====	+	==Crystal structure of a OTU deubiquitinase in complex with Ub-PA==
-	<StructureSection load='7bu0' size='340' side='right'caption='[[7bu0]]' scene=''>	+	<StructureSection load='7bu0' size='340' side='right'caption='[[7bu0]], [[Resolution\|resolution]] 2.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bu0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BU0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7bu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bu0 OCA], [~~http~~://pdbe.org/7bu0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7bu0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7bu0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7bu0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AYE:PROP-2-EN-1-AMINE'>AYE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bu0 OCA], [https://pdbe.org/7bu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bu0 RCSB], [https://www.ebi.ac.uk/pdbsum/7bu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bu0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5ZSI8_LEGPH Q5ZSI8_LEGPH]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Legionella pneumophila extensively modulates the host ubiquitin network to create the Legionella-containing vacuole (LCV) for its replication. Many of its virulence factors function as ubiquitin ligases or deubiquitinases (DUBs). Here, we identify Lem27 as a DUB that displays a preference for diubiquitin formed by K6, K11, or K48. Lem27 is associated with the LCV where it regulates Rab10 ubiquitination in concert with SidC and SdcA, two bacterial E3 ubiquitin ligases. Structural analysis of the complex formed by an active fragment of Lem27 and the substrate-based suicide inhibitor ubiquitin-propargylamide (PA) reveals that it harbors a fold resembling those in the OTU1 DUB subfamily with a Cys-His catalytic dyad and that it recognizes ubiquitin via extensive hydrogen bonding at six contact sites. Our results establish Lem27 as a DUB that functions to regulate protein ubiquitination on L. pneumophila phagosomes by counteracting the activity of bacterial ubiquitin E3 ligases.
		+
		+	Interplay between bacterial deubiquitinase and ubiquitin E3 ligase regulates ubiquitin dynamics on Legionella phagosomes.,Liu S, Luo J, Zhen X, Qiu J, Ouyang S, Luo ZQ Elife. 2020 Nov 2;9:e58114. doi: 10.7554/eLife.58114. PMID:33136002<ref>PMID:33136002</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bu0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
		+	[[Category: Ouyang SY]]
		+	[[Category: Zhen XK]]

OCA at 09:40, 18 November 2020

OCA — Wed, 18 Nov 2020 09:40:41 GMT

←Older revision		Revision as of 09:40, 18 November 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7bu0 ~~is ON HOLD until Apr 03 2022~~	+	====
-		+	<StructureSection load='7bu0' size='340' side='right'caption='[[7bu0]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bu0 OCA], [http://pdbe.org/7bu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bu0 RCSB], [http://www.ebi.ac.uk/pdbsum/7bu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bu0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:50, 6 May 2020

OCA — Wed, 06 May 2020 04:50:57 GMT

←Older revision		Revision as of 04:50, 6 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bu0 is ON HOLD	+	The entry 7bu0 is ON HOLD until Apr 03 2022

	Authors:		Authors:

OCA: Protected "7bu0" [edit=sysop:move=sysop]

OCA — Wed, 08 Apr 2020 05:53:48 GMT

Protected "7bu0" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:53, 8 April 2020

OCA: New page: '''Unreleased structure''' The entry 7bu0 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Apr 2020 05:53:47 GMT

New page: '''Unreleased structure''' The entry 7bu0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bu0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7bu0 - Revision history

OCA at 06:36, 7 April 2023

OCA at 09:40, 18 November 2020

OCA at 04:50, 6 May 2020

OCA: Protected "7bu0" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7bu0 is ON HOLD Authors: Description: Category: Unreleased Structures