7bvl - Revision history

OCA at 10:05, 6 September 2023

OCA — Wed, 06 Sep 2023 10:05:22 GMT

←Older revision		Revision as of 10:05, 6 September 2023
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	==Crystal structure of glucose isomerase delivered in wheat starch==		==Crystal structure of glucose isomerase delivered in wheat starch==
-	<StructureSection load='7bvl' size='340' side='right'caption='[[7bvl]]' scene=''>	+	<StructureSection load='7bvl' size='340' side='right'caption='[[7bvl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7BVL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7bvl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BVL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7bvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvl OCA], [~~http~~://pdbe.org/7bvl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7bvl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7bvl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvl OCA], [https://pdbe.org/7bvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bvl RCSB], [https://www.ebi.ac.uk/pdbsum/7bvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serial crystallography (SX) provides an opportunity to observe the molecular dynamics of macromolecular structures at room temperature via pump-probe studies. The delivery of crystals embedded in a viscous medium via an injector or syringe is widely performed in synchrotrons or X-ray free-electron laser facilities with low repetition rates. Various viscous media have been developed; however, there are cases in which the delivery material undesirably interacts chemically or biologically with specific protein samples, or changes the stability of the injection stream, depending on the crystallization solution. Therefore, continued discovery and characterization of new delivery media is necessary for expanding future SX applications. Here, the preparation and characterization of new polysaccharide (wheat starch (WS) and alginate)-based sample delivery media are introduced for SX. Crystals embedded in a WS or alginate injection medium showed a stable injection stream at a flow rate of < 200 nL/min and low-level X-ray background scattering similar to other hydrogels. Using these media, serial millisecond crystallography (SMX) was performed, and the room temperature crystal structures of glucose isomerase and lysozyme were determined at 1.9-2.0 A resolutions. WS and alginate will allow an expanded application of sample delivery media in SX experiments.
		+
		+	Polysaccharide-Based Injection Matrix for Serial Crystallography.,Nam KH Int J Mol Sci. 2020 May 8;21(9). pii: ijms21093332. doi: 10.3390/ijms21093332. PMID:32397185<ref>PMID:32397185</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7bvl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[D-xylose isomerase 3D structures\|D-xylose isomerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptomyces rubiginosus]]
	[[Category: Nam KH]]		[[Category: Nam KH]]

OCA at 09:40, 18 November 2020

OCA — Wed, 18 Nov 2020 09:40:44 GMT

←Older revision		Revision as of 09:40, 18 November 2020
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-	'''Unreleased structure'''

-	~~The entry 7bvl is ON HOLD until Paper Publication~~	+	==Crystal structure of glucose isomerase delivered in wheat starch==
-		+	<StructureSection load='7bvl' size='340' side='right'caption='[[7bvl]]' scene=''>
-	~~Authors: Nam, K.H.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BVL FirstGlance]. <br>
-	~~Description:~~ Crystal structure of glucose isomerase delivered in wheat starch	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvl OCA], [http://pdbe.org/7bvl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bvl RCSB], [http://www.ebi.ac.uk/pdbsum/7bvl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Nam~~, K.H~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Nam KH]]

OCA at 05:24, 3 June 2020

OCA — Wed, 03 Jun 2020 05:24:07 GMT

←Older revision		Revision as of 05:24, 3 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7bvl is ON HOLD	+	The entry 7bvl is ON HOLD until Paper Publication

	Authors: Nam, K.H.		Authors: Nam, K.H.

OCA: Protected "7bvl" [edit=sysop:move=sysop]

OCA — Wed, 22 Apr 2020 04:36:18 GMT

Protected "7bvl" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 22 April 2020

OCA: New page: '''Unreleased structure''' The entry 7bvl is ON HOLD Authors: Nam, K.H. Description: Crystal structure of glucose isomerase delivered in wheat starch [[Category: Unreleased Structures]...

OCA — Wed, 22 Apr 2020 04:36:17 GMT

New page: '''Unreleased structure''' The entry 7bvl is ON HOLD Authors: Nam, K.H. Description: Crystal structure of glucose isomerase delivered in wheat starch [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 7bvl is ON HOLD

Authors: Nam, K.H.

Description: Crystal structure of glucose isomerase delivered in wheat starch
[[Category: Unreleased Structures]]
[[Category: Nam, K.H]]