OCA at 15:40, 29 November 2023

OCA — Wed, 29 Nov 2023 15:40:19 GMT

←Older revision		Revision as of 15:40, 29 November 2023
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	==Tetanus neurotoxin receptor binding domain==		==Tetanus neurotoxin receptor binding domain==
-	<StructureSection load='7by4' size='340' side='right'caption='[[7by4]]' scene=''>	+	<StructureSection load='7by4' size='340' side='right'caption='[[7by4]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BY4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7by4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_tetani_E88 Clostridium tetani E88]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BY4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7by4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7by4 OCA], [https://pdbe.org/7by4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7by4 RCSB], [https://www.ebi.ac.uk/pdbsum/7by4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7by4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7by4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7by4 OCA], [https://pdbe.org/7by4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7by4 RCSB], [https://www.ebi.ac.uk/pdbsum/7by4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7by4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TETX_CLOTE TETX_CLOTE] Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although the tetanus neurotoxin (TeNT) delivers its protease domain (LC) across the synaptic vesicle lumen into the cytosol via its receptor binding domain (H(C)) and translocation domain (H(N)), the molecular mechanism coordinating this membrane translocation remains unresolved. Here, we report the high-resolution crystal structures of full-length reduced TeNT (rTeNT, 2.3 A), TeNT isolated H(N) (TeNT/iH(N), 2.3 A), TeNT isolated H(C) (TeNT/iH(C), 1.5 A), together with the solution structures of TeNT/iH(N) and beltless TeNT/iH(N) (TeNT/blH(N)). TeNT undergoes significant domains rotation of the H(N) and LC were demonstrated by structural comparison of rTeNT and non-reduced-TeNT (nrTeNT). A linker loop connects the H(N) and H(C) is essential for the self-domain rotation of TeNT. The TeNT-specific C869-C1093 disulfide bond is sensitive to the redox environment and its disruption provides linker loop flexibility, which enables domain arrangement of rTeNT distinct from that of nrTeNT. Furthermore, the mobility of C869 in the linker loop and the sensitivity to redox condition of C1093 were confirmed by crystal structure analysis of TeNT/iH(C). On the other hand, the structural flexibility of H(N) was investigated by crystallographic and solution scattering analyses. It was found that the region (residues 698-769), which follows the translocation region had remarkable change in TeNT/iH(N). Besides, the so-called belt region has a high propensity to swing around the upper half of TeNT/iH(N) at acidic pH. It provides the first overview of the dynamics of the Belt in solution. These newly obtained structural information that shed light on the transmembrane mechanism of TeNT.
		+
		+	Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses.,Zhang CM, Imoto Y, Hikima T, Inoue T J Struct Biol X. 2021 Jan 30;5:100045. doi: 10.1016/j.yjsbx.2021.100045. , eCollection 2021. PMID:33598655<ref>PMID:33598655</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7by4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetanus toxin\|Tetanus toxin]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridium tetani E88]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Imoto Y]]		[[Category: Imoto Y]]
	[[Category: Inoue T]]		[[Category: Inoue T]]
	[[Category: Zhang CM]]		[[Category: Zhang CM]]

OCA at 10:01, 17 February 2021

OCA — Wed, 17 Feb 2021 10:01:41 GMT

←Older revision		Revision as of 10:01, 17 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7by4 is ~~ON HOLD until Paper Publication~~	+	==Tetanus neurotoxin receptor binding domain==
-		+	<StructureSection load='7by4' size='340' side='right'caption='[[7by4]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BY4 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7by4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7by4 OCA], [https://pdbe.org/7by4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7by4 RCSB], [https://www.ebi.ac.uk/pdbsum/7by4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7by4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Imoto Y]]
		+	[[Category: Inoue T]]
		+	[[Category: Zhang CM]]

OCA at 07:25, 9 September 2020

OCA — Wed, 09 Sep 2020 07:25:11 GMT

←Older revision		Revision as of 07:25, 9 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7by4 is ON HOLD	+	The entry 7by4 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 08:22, 22 July 2020

OCA — Wed, 22 Jul 2020 08:22:36 GMT

←Older revision		Revision as of 08:22, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7by4 is ON HOLD ~~until Paper Publication~~	+	The entry 7by4 is ON HOLD

	Authors:		Authors:

OCA at 05:08, 27 May 2020

OCA — Wed, 27 May 2020 05:08:54 GMT

←Older revision		Revision as of 05:08, 27 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7by4 is ON HOLD	+	The entry 7by4 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7by4" [edit=sysop:move=sysop]

OCA — Wed, 29 Apr 2020 05:37:39 GMT

Protected "7by4" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 29 April 2020

OCA: New page: '''Unreleased structure''' The entry 7by4 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Apr 2020 05:37:37 GMT

New page: '''Unreleased structure''' The entry 7by4 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7by4 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]