7c1j - Revision history

OCA at 15:49, 29 November 2023

OCA — Wed, 29 Nov 2023 15:49:48 GMT

←Older revision		Revision as of 15:49, 29 November 2023
Line 1:		Line 1:

	==Crystal structure of the receiver domain of sensor histidine kinase PA1611 (PA1611REC) from Pseudomonas aeruginosa PAO1 with magnesium ion coordinated in the active site cleft==		==Crystal structure of the receiver domain of sensor histidine kinase PA1611 (PA1611REC) from Pseudomonas aeruginosa PAO1 with magnesium ion coordinated in the active site cleft==
-	<StructureSection load='7c1j' size='340' side='right'caption='[[7c1j]]' scene=''>	+	<StructureSection load='7c1j' size='340' side='right'caption='[[7c1j]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C1J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7C1J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7c1j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C1J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7c1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c1j OCA], [~~http~~://pdbe.org/7c1j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7c1j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7c1j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7c1j ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.35Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c1j OCA], [https://pdbe.org/7c1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c1j RCSB], [https://www.ebi.ac.uk/pdbsum/7c1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c1j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9I3B1_PSEAE Q9I3B1_PSEAE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Pseudomonas aeruginosa, an important opportunistic pathogen that causes numerous acute and chronic infections, the hybrid two-component system (TCS) regulates the swarming ability and biofilm formation with a multistep phospho-relay, and consists of hybrid-sensor histidine kinase (HK), histidine-containing phospho-transfer protein (Hpt) and response regulator (RR). In this work, two crystal structures of HptB and the receiver domain of HK PA1611 (PA1611REC) of P. aeruginosa have been determined in order to elucidate their interactions for the transfer of the phospho-ryl group. The structure of HptB folds into an elongated four-helix bundle - helices alpha2, alpha3, alpha4 and alpha5, covered by the short N-terminal helix alpha1. The imidazole side chain of the conserved active-site histidine residue His57, located near the middle of helix alpha3, protrudes from the bundle and is exposed to solvent. The structure of PA1611REC possesses a conventional (beta/alpha)5 topology with five-stranded parallel beta-sheets folded in the central region, surrounded by five alpha-helices. The divalent Mg(2+) ion is located in the negatively charged active-site cleft and interacts with Asp522, Asp565 and Arg567. The HptB-PA1611REC complex is further modeled to analyze the binding surface and interactions between the two proteins. The model shows a shape complementarity between the convex surface of PA1611REC and the kidney-shaped HptB with fewer residues and a different network involved in interactions compared with other TCS complexes, such as SLN1-R1/YPD1 from Saccharomyces cerevisiae and AHK5RD/AHP1 from Arabidopsis thaliana. These structural results provide a better understanding of the TCS in P. aeruginosa and could potentially lead to the discovery of a new treatment for infection.
		+
		+	Structural insights into the histidine-containing phospho-transfer protein and receiver domain of sensor histidine kinase suggest a complex model in the two-component regulatory system in Pseudomonas aeruginosa.,Chen SK, Guan HH, Wu PH, Lin LT, Wu MC, Chang HY, Chen NC, Lin CC, Chuankhayan P, Huang YC, Lin PJ, Chen CJ IUCrJ. 2020 Aug 25;7(Pt 5):934-948. doi: 10.1107/S2052252520009665. eCollection, 2020 Sep 1. PMID:32939285<ref>PMID:32939285</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7c1j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa]]
	[[Category: Chang HY]]		[[Category: Chang HY]]
	[[Category: Chen CJ]]		[[Category: Chen CJ]]

OCA at 07:25, 4 November 2020

OCA — Wed, 04 Nov 2020 07:25:51 GMT

←Older revision		Revision as of 07:25, 4 November 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7c1j is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the receiver domain of sensor histidine kinase PA1611 (PA1611REC) from Pseudomonas aeruginosa PAO1 with magnesium ion coordinated in the active site cleft==
-		+	<StructureSection load='7c1j' size='340' side='right'caption='[[7c1j]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C1J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C1J FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c1j OCA], [http://pdbe.org/7c1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c1j RCSB], [http://www.ebi.ac.uk/pdbsum/7c1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c1j ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chang HY]]
		+	[[Category: Chen CJ]]
		+	[[Category: Chen NC]]
		+	[[Category: Chen SK]]
		+	[[Category: Chuankhayan P]]
		+	[[Category: Guan HH]]
		+	[[Category: Huang YC]]
		+	[[Category: Lin CC]]
		+	[[Category: Lin LT]]
		+	[[Category: Lin PJ]]
		+	[[Category: Wu MC]]
		+	[[Category: Wu PH]]

OCA at 04:05, 5 August 2020

OCA — Wed, 05 Aug 2020 04:05:50 GMT

←Older revision		Revision as of 04:05, 5 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7c1j is ON HOLD	+	The entry 7c1j is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 08:22, 22 July 2020

OCA — Wed, 22 Jul 2020 08:22:55 GMT

←Older revision		Revision as of 08:22, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7c1j is ON HOLD ~~until Paper Publication~~	+	The entry 7c1j is ON HOLD

	Authors:		Authors:

OCA at 05:14, 10 June 2020

OCA — Wed, 10 Jun 2020 05:14:42 GMT

←Older revision		Revision as of 05:14, 10 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7c1j is ON HOLD	+	The entry 7c1j is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7c1j" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2020 04:23:48 GMT

Protected "7c1j" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:23, 13 May 2020

OCA: New page: '''Unreleased structure''' The entry 7c1j is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 May 2020 04:23:47 GMT

New page: '''Unreleased structure''' The entry 7c1j is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7c1j is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]