7c3t - Revision history

OCA at 15:54, 29 November 2023

OCA — Wed, 29 Nov 2023 15:54:58 GMT

←Older revision		Revision as of 15:54, 29 November 2023
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	<StructureSection load='7c3t' size='340' side='right'caption='[[7c3t]], [[Resolution\|resolution]] 2.07Å' scene=''>		<StructureSection load='7c3t' size='340' side='right'caption='[[7c3t]], [[Resolution\|resolution]] 2.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7c3t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Niteu Niteu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C3T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7c3t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea_ATCC_19718 Nitrosomonas europaea ATCC 19718]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C3T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZG:5-AMINO-1H-[1,2,3]TRIAZOLO[4,5-D]PYRIMIDIN-7-OL'>AZG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.07Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NE0047 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 NITEU])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZG:5-AMINO-1H-[1,2,3]TRIAZOLO[4,5-D]PYRIMIDIN-7-OL'>AZG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c3t OCA], [https://pdbe.org/7c3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c3t RCSB], [https://www.ebi.ac.uk/pdbsum/7c3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c3t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c3t OCA], [https://pdbe.org/7c3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c3t RCSB], [https://www.ebi.ac.uk/pdbsum/7c3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c3t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q82Y41_NITEU Q82Y41_NITEU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7c3t" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7c3t" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Deaminase 3D structures\|Deaminase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Niteu~~]]	+	[[Category: Nitrosomonas europaea ATCC 19718]]
-	[[Category: Anand, R]]	+	[[Category: Anand R]]
-	[[Category: Bitra, A]]	+	[[Category: Bitra A]]
-	[[Category: Gaded, V]]	+	[[Category: Gaded V]]
-	[[Category: Singh, J]]	+	[[Category: Singh J]]
-	~~[[Category: 8-azaguanine]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Deaminase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 14:49, 2 June 2021

OCA — Wed, 02 Jun 2021 14:49:27 GMT

←Older revision		Revision as of 14:49, 2 June 2021
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	==Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine==		==Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine==
-	<StructureSection load='7c3t' size='340' side='right'caption='[[7c3t]]' scene=''>	+	<StructureSection load='7c3t' size='340' side='right'caption='[[7c3t]], [[Resolution\|resolution]] 2.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C3T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7c3t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Niteu Niteu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C3T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c3t OCA], [https://pdbe.org/7c3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c3t RCSB], [https://www.ebi.ac.uk/pdbsum/7c3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c3t ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZG:5-AMINO-1H-[1,2,3]TRIAZOLO[4,5-D]PYRIMIDIN-7-OL'>AZG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NE0047 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 NITEU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c3t OCA], [https://pdbe.org/7c3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c3t RCSB], [https://www.ebi.ac.uk/pdbsum/7c3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c3t ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Guanine deaminases (GDs) are essential enzymes that regulate the overall nucleobase pool. Since the deamination of guanine to xanthine results in the production of a mutagenic base, these enzymes have evolved to be very specific in nature. Surprisingly, they accept structurally distinct triazine ammeline, an intermediate in the melamine pathway, as one of the moonlighting substrates. Here, by employing NE0047 (a GD from Nitrosomonas europaea), we delineate the nuance in the catalytic mechanism that allows these two distinct substrates to be catalyzed. A combination of enzyme kinetics, X-ray crystallographic, and calorimetric studies reveal that GDs operate via a dual proton shuttle mechanism with two glutamates, E79 and E143, crucial for deamination. Additionally, N66 appears to be central for substrate anchoring and participates in catalysis. The study highlights the importance of closure of the catalytic loop and of maintenance of the hydrophobic core by capping residues like F141 and F48 for the creation of an apt environment for activation of the zinc-assisted catalysis. This study also analyzes evolutionarily distinct GDs and asserts that GDs incorporate subtle variations in the active site architectures while keeping the most critical active site determinants conserved.
		+
		+	Structure Guided Mutagenesis Reveals the Substrate Determinants of Guanine Deaminase.,Singh J, Gaded V, Bitra A, Anand R J Struct Biol. 2021 May 16:107747. doi: 10.1016/j.jsb.2021.107747. PMID:34010666<ref>PMID:34010666</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7c3t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Anand R]]	+	[[Category: Niteu]]
-	[[Category: Bitra A]]	+	[[Category: Anand, R]]
-	[[Category: Gaded V]]	+	[[Category: Bitra, A]]
-	[[Category: Singh J]]	+	[[Category: Gaded, V]]
		+	[[Category: Singh, J]]
		+	[[Category: 8-azaguanine]]
		+	[[Category: Complex]]
		+	[[Category: Deaminase]]
		+	[[Category: Hydrolase]]

OCA at 09:18, 26 May 2021

OCA — Wed, 26 May 2021 09:18:08 GMT

←Older revision		Revision as of 09:18, 26 May 2021
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-	'''Unreleased structure'''

-	~~The entry 7c3t is ON HOLD until Paper Publication~~	+	==Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine==
-		+	<StructureSection load='7c3t' size='340' side='right'caption='[[7c3t]]' scene=''>
-	~~Authors: Gaded, V., Bitra, A., Singh, J., Anand, R.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C3T FirstGlance]. <br>
-	~~Description:~~ Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c3t OCA], [https://pdbe.org/7c3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c3t RCSB], [https://www.ebi.ac.uk/pdbsum/7c3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c3t ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Bitra~~, A]]	+	__TOC__
-	[[Category: Anand, R]]	+	</StructureSection>
-	[[Category: ~~Singh, J~~]]	+	[[Category: Large Structures]]
-	[[Category: Gaded, V]]	+	[[Category: Anand R]]
		+	[[Category: Bitra A]]
		+	[[Category: Gaded V]]
		+	[[Category: Singh J]]

OCA at 07:13, 5 May 2021

OCA — Wed, 05 May 2021 07:13:56 GMT

←Older revision		Revision as of 07:13, 5 May 2021
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	The entry 7c3t is ON HOLD until Paper Publication		The entry 7c3t is ON HOLD until Paper Publication

-	Authors: Gaded, V., Bitra, A., Anand, R.	+	Authors: Gaded, V., Bitra, A., Singh, J., Anand, R.

	Description: Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine		Description: Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine
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	[[Category: Bitra, A]]		[[Category: Bitra, A]]
	[[Category: Anand, R]]		[[Category: Anand, R]]
		+	[[Category: Singh, J]]
	[[Category: Gaded, V]]		[[Category: Gaded, V]]

OCA at 05:25, 3 June 2020

OCA — Wed, 03 Jun 2020 05:25:31 GMT

←Older revision		Revision as of 05:25, 3 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7c3t is ON HOLD	+	The entry 7c3t is ON HOLD until Paper Publication

	Authors: Gaded, V., Bitra, A., Anand, R.		Authors: Gaded, V., Bitra, A., Anand, R.
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	Description: Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine		Description: Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Bitra, A]]
	[[Category: Anand, R]]		[[Category: Anand, R]]
	[[Category: Gaded, V]]		[[Category: Gaded, V]]
-	[[Category: Bitra, A]]

OCA: Protected "7c3t" [edit=sysop:move=sysop]

OCA — Wed, 20 May 2020 04:41:22 GMT

Protected "7c3t" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:41, 20 May 2020

OCA: New page: '''Unreleased structure''' The entry 7c3t is ON HOLD Authors: Gaded, V., Bitra, A., Anand, R. Description: Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine [[Cate...

OCA — Wed, 20 May 2020 04:41:21 GMT

New page: '''Unreleased structure''' The entry 7c3t is ON HOLD Authors: Gaded, V., Bitra, A., Anand, R. Description: Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine [[Cate...

New page

'''Unreleased structure'''

The entry 7c3t is ON HOLD

Authors: Gaded, V., Bitra, A., Anand, R.

Description: Crystal structure of NE0047 (N66Q) mutant in complex with 8-azaguanine
[[Category: Unreleased Structures]]
[[Category: Anand, R]]
[[Category: Gaded, V]]
[[Category: Bitra, A]]