7c8x - Revision history

OCA at 16:00, 29 November 2023

OCA — Wed, 29 Nov 2023 16:00:36 GMT

←Older revision		Revision as of 16:00, 29 November 2023
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	==Blasnase-T13A with L-asn==		==Blasnase-T13A with L-asn==
-	<StructureSection load='7c8x' size='340' side='right'caption='[[7c8x]]' scene=''>	+	<StructureSection load='7c8x' size='340' side='right'caption='[[7c8x]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C8X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7c8x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_paralicheniformis Bacillus paralicheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C8X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8x OCA], [https://pdbe.org/7c8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8x RCSB], [https://www.ebi.ac.uk/pdbsum/7c8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.994Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8x OCA], [https://pdbe.org/7c8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8x RCSB], [https://www.ebi.ac.uk/pdbsum/7c8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8x ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	l-Asparaginase, which catalyzes the hydrolysis of l-asparagine, is an important enzyme in both the clinical and food industry. Exploration of efficient l-asparaginase with high substrate specificity, especially high chiral selectivity, is essential for extending its use. Herein, various crystal structures of type I l-asparaginase from Bacillus licheniformis (BlAsnase) have been resolved, and we found that there are two additional tyrosines in BlAsnase, contributing to the binding and catalysis of d-asparagine. Strikingly, the substitution of Tyr278 with methionine impaired the interaction with d-asparagine via water molecules due to the small hydrophobic side chain of methionine, which forced the ligand to the deep side of the active site toward the catalytic residues and thus resulted in the loss of hydrolyzing function. Our investigation of the substrate recognition mechanism of BlAsnase is significant for both a better understanding of l-asparaginase and its rational design to achieve high specificity for clinical and industrial applications.
		+
		+	Structures of l-asparaginase from Bacillus licheniformis Reveal an Essential Residue for its Substrate Stereoselectivity.,Ran T, Jiao L, Wang W, Chen J, Chi H, Lu Z, Zhang C, Xu D, Lu F J Agric Food Chem. 2021 Jan 13;69(1):223-231. doi: 10.1021/acs.jafc.0c06609. Epub, 2020 Dec 28. PMID:33371681<ref>PMID:33371681</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7c8x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Asparaginase 3D structures\|Asparaginase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus paralicheniformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jiao L]]		[[Category: Jiao L]]

OCA at 12:12, 9 June 2021

OCA — Wed, 09 Jun 2021 12:12:14 GMT

←Older revision		Revision as of 12:12, 9 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7c8x ~~is ON HOLD until Paper Publication~~	+	==Blasnase-T13A with L-asn==
-		+	<StructureSection load='7c8x' size='340' side='right'caption='[[7c8x]]' scene=''>
-	~~Authors~~: Lu, F., ~~Ran~~, T., ~~Jiao, L~~., ~~Wang, W~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C8X FirstGlance]. <br>
-	~~Description: Blasnase-T13A with L-asn~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8x OCA], [https://pdbe.org/7c8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8x RCSB], [https://www.ebi.ac.uk/pdbsum/7c8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8x ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Jiao, L]]	+	__TOC__
-	[[Category: ~~Wang, W~~]]	+	</StructureSection>
-	[[Category: Ran, T]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lu, F~~]]	+	[[Category: Jiao L]]
		+	[[Category: Lu F]]
		+	[[Category: Ran T]]
		+	[[Category: Wang W]]

OCA at 04:26, 7 April 2021

OCA — Wed, 07 Apr 2021 04:26:58 GMT

←Older revision		Revision as of 04:26, 7 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7c8x is ON HOLD	+	The entry 7c8x is ON HOLD until Paper Publication

	Authors: Lu, F., Ran, T., Jiao, L., Wang, W.		Authors: Lu, F., Ran, T., Jiao, L., Wang, W.

OCA: Protected "7c8x" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 05:19:19 GMT

Protected "7c8x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:19, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 7c8x is ON HOLD Authors: Lu, F., Ran, T., Jiao, L., Wang, W. Description: Blasnase-T13A with L-asn Category: Unreleased Structures [[Category: ...

OCA — Wed, 10 Jun 2020 05:19:18 GMT

New page: '''Unreleased structure''' The entry 7c8x is ON HOLD Authors: Lu, F., Ran, T., Jiao, L., Wang, W. Description: Blasnase-T13A with L-asn Category: Unreleased Structures [[Category: ...

New page

'''Unreleased structure'''

The entry 7c8x is ON HOLD

Authors: Lu, F., Ran, T., Jiao, L., Wang, W.

Description: Blasnase-T13A with L-asn
[[Category: Unreleased Structures]]
[[Category: Jiao, L]]
[[Category: Wang, W]]
[[Category: Ran, T]]
[[Category: Lu, F]]