7cb4 - Revision history

OCA at 16:01, 29 November 2023

OCA — Wed, 29 Nov 2023 16:01:52 GMT

←Older revision		Revision as of 16:01, 29 November 2023
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	==Crystal structures of of BlAsnase==		==Crystal structures of of BlAsnase==
-	<StructureSection load='7cb4' size='340' side='right'caption='[[7cb4]]' scene=''>	+	<StructureSection load='7cb4' size='340' side='right'caption='[[7cb4]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CB4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cb4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_paralicheniformis Bacillus paralicheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CB4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cb4 OCA], [https://pdbe.org/7cb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cb4 RCSB], [https://www.ebi.ac.uk/pdbsum/7cb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cb4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.04Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cb4 OCA], [https://pdbe.org/7cb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cb4 RCSB], [https://www.ebi.ac.uk/pdbsum/7cb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cb4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6I7U6Y2_9BACI A0A6I7U6Y2_9BACI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	l-Asparaginase, which catalyzes the hydrolysis of l-asparagine, is an important enzyme in both the clinical and food industry. Exploration of efficient l-asparaginase with high substrate specificity, especially high chiral selectivity, is essential for extending its use. Herein, various crystal structures of type I l-asparaginase from Bacillus licheniformis (BlAsnase) have been resolved, and we found that there are two additional tyrosines in BlAsnase, contributing to the binding and catalysis of d-asparagine. Strikingly, the substitution of Tyr278 with methionine impaired the interaction with d-asparagine via water molecules due to the small hydrophobic side chain of methionine, which forced the ligand to the deep side of the active site toward the catalytic residues and thus resulted in the loss of hydrolyzing function. Our investigation of the substrate recognition mechanism of BlAsnase is significant for both a better understanding of l-asparaginase and its rational design to achieve high specificity for clinical and industrial applications.
		+
		+	Structures of l-asparaginase from Bacillus licheniformis Reveal an Essential Residue for its Substrate Stereoselectivity.,Ran T, Jiao L, Wang W, Chen J, Chi H, Lu Z, Zhang C, Xu D, Lu F J Agric Food Chem. 2021 Jan 13;69(1):223-231. doi: 10.1021/acs.jafc.0c06609. Epub, 2020 Dec 28. PMID:33371681<ref>PMID:33371681</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cb4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Asparaginase 3D structures\|Asparaginase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus paralicheniformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jiao L]]		[[Category: Jiao L]]

OCA at 12:12, 9 June 2021

OCA — Wed, 09 Jun 2021 12:12:28 GMT

←Older revision		Revision as of 12:12, 9 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7cb4 ~~is ON HOLD~~	+	==Crystal structures of of BlAsnase==
-		+	<StructureSection load='7cb4' size='340' side='right'caption='[[7cb4]]' scene=''>
-	~~Authors~~: Lu, F., ~~Ran~~, T., ~~Jiao, L~~., ~~Wang, W~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CB4 FirstGlance]. <br>
-	~~Description: Crystal structures of of BlAsnase~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cb4 OCA], [https://pdbe.org/7cb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cb4 RCSB], [https://www.ebi.ac.uk/pdbsum/7cb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cb4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Jiao, L]]	+	__TOC__
-	[[Category: ~~Wang, W~~]]	+	</StructureSection>
-	[[Category: Ran, T]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lu, F~~]]	+	[[Category: Jiao L]]
		+	[[Category: Lu F]]
		+	[[Category: Ran T]]
		+	[[Category: Wang W]]

OCA at 05:32, 14 April 2021

OCA — Wed, 14 Apr 2021 05:32:22 GMT

←Older revision		Revision as of 05:32, 14 April 2021
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	Authors: Lu, F., Ran, T., Jiao, L., Wang, W.		Authors: Lu, F., Ran, T., Jiao, L., Wang, W.

-	Description: Crystal structures of of ~~Blasnase~~	+	Description: Crystal structures of of BlAsnase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Jiao, L]]		[[Category: Jiao, L]]
	[[Category: Wang, W]]		[[Category: Wang, W]]
-	[[Category: Lu, F]]
	[[Category: Ran, T]]		[[Category: Ran, T]]
		+	[[Category: Lu, F]]

OCA at 09:12, 31 March 2021

OCA — Wed, 31 Mar 2021 09:12:34 GMT

←Older revision		Revision as of 09:12, 31 March 2021
Line 5:		Line 5:
	Authors: Lu, F., Ran, T., Jiao, L., Wang, W.		Authors: Lu, F., Ran, T., Jiao, L., Wang, W.

-	Description: Blasnase~~-T13A with D-asn~~	+	Description: Crystal structures of of Blasnase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Jiao, L]]		[[Category: Jiao, L]]
	[[Category: Wang, W]]		[[Category: Wang, W]]
-	[[Category: Ran, T]]
	[[Category: Lu, F]]		[[Category: Lu, F]]
		+	[[Category: Ran, T]]

OCA: Protected "7cb4" [edit=sysop:move=sysop]

OCA — Wed, 17 Jun 2020 09:03:33 GMT

Protected "7cb4" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:03, 17 June 2020

OCA: New page: '''Unreleased structure''' The entry 7cb4 is ON HOLD Authors: Lu, F., Ran, T., Jiao, L., Wang, W. Description: Blasnase-T13A with D-asn Category: Unreleased Structures [[Category: ...

OCA — Wed, 17 Jun 2020 09:03:32 GMT

New page: '''Unreleased structure''' The entry 7cb4 is ON HOLD Authors: Lu, F., Ran, T., Jiao, L., Wang, W. Description: Blasnase-T13A with D-asn Category: Unreleased Structures [[Category: ...

New page

'''Unreleased structure'''

The entry 7cb4 is ON HOLD

Authors: Lu, F., Ran, T., Jiao, L., Wang, W.

Description: Blasnase-T13A with D-asn
[[Category: Unreleased Structures]]
[[Category: Jiao, L]]
[[Category: Wang, W]]
[[Category: Ran, T]]
[[Category: Lu, F]]