7ce3 - Revision history

OCA at 16:06, 29 November 2023

OCA — Wed, 29 Nov 2023 16:06:38 GMT

←Older revision		Revision as of 16:06, 29 November 2023
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-	====	+	==Crystal structure of human IDH3 holoenzyme in APO form.==
-	<StructureSection load='7ce3' size='340' side='right'caption='[[7ce3]]' scene=''>	+	<StructureSection load='7ce3' size='340' side='right'caption='[[7ce3]], [[Resolution\|resolution]] 3.47Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ce3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CE3 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7ce3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ce3 OCA], [~~http~~://pdbe.org/7ce3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7ce3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7ce3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7ce3 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.472Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ce3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ce3 OCA], [https://pdbe.org/7ce3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ce3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ce3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ce3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IDH3G_HUMAN IDH3G_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human NAD-dependent isocitrate dehydrogenase or HsIDH3 catalyzes the decarboxylation of isocitrate into alpha-ketoglutarate in the TCA cycle. HsIDH3 exists and functions as a heterooctamer composed of the alphabeta and alphagamma heterodimers, and is regulated allosterically and/or competitively by numerous metabolites including CIT, ADP, ATP, and NADH. In this work, we report the crystal structure of HsIDH3 containing a beta mutant in apo form. In the HsIDH3 structure, the alphabeta and alphagamma heterodimers form the alpha(2)betagamma heterotetramer via their clasp domains, and two alpha(2)betagamma heterotetramers form the (alpha(2)betagamma)(2) heterooctamer through insertion of the N-terminus of the gamma subunit of one heterotetramer into the back cleft of the beta subunit of the other heterotetramer. The functional roles of the key residues at the allosteric site, the pseudo allosteric site, the heterodimer and heterodimer-heterodimer interfaces, and the N-terminal of the gamma subunit are validated by mutagenesis and kinetic studies. Our structural and biochemical data together demonstrate that the allosteric site plays an important role but the pseudo allosteric site plays no role in the allosteric activation of the enzyme; the activation signal from the allosteric site is transmitted to the active sites of both alphabeta and alphagamma heterodimers via the clasp domains; and the N-terminal of the gamma subunit plays a critical role in the formation of the heterooctamer to ensure the optimal activity of the enzyme. These findings reveal the molecular mechanism of the assembly and allosteric regulation of HsIDH3.
		+
		+	Structure and allosteric regulation of human NAD-dependent isocitrate dehydrogenase.,Sun P, Liu Y, Ma T, Ding J Cell Discov. 2020 Dec 22;6(1):94. doi: 10.1038/s41421-020-00220-7. PMID:33349631<ref>PMID:33349631</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ce3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Isocitrate dehydrogenase 3D structures\|Isocitrate dehydrogenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Ding JP]]
		+	[[Category: Sun PK]]

OCA at 08:54, 6 January 2021

OCA — Wed, 06 Jan 2021 08:54:21 GMT

←Older revision		Revision as of 08:54, 6 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7ce3 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ce3' size='340' side='right'caption='[[7ce3]]' scene=''>
-	~~Authors~~: ~~Sun, P~~.K.~~, Ding, J~~.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal~~ structure ~~of human IDH3 holoenzyme in APO form~~.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7ce3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ce3 OCA], [http://pdbe.org/7ce3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7ce3 RCSB], [http://www.ebi.ac.uk/pdbsum/7ce3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7ce3 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Sun~~, P.K]]	+	__TOC__
-	[[Category: ~~Ding, J.P~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:24, 22 July 2020

OCA — Wed, 22 Jul 2020 08:24:43 GMT

←Older revision		Revision as of 08:24, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ce3 is ON HOLD	+	The entry 7ce3 is ON HOLD until Paper Publication

	Authors: Sun, P.K., Ding, J.P.		Authors: Sun, P.K., Ding, J.P.

OCA: Protected "7ce3" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 11:26:51 GMT

Protected "7ce3" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:26, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 7ce3 is ON HOLD Authors: Sun, P.K., Ding, J.P. Description: Crystal structure of human IDH3 holoenzyme in APO form. [[Category: Unreleased Structur...

OCA — Wed, 08 Jul 2020 11:26:49 GMT

New page: '''Unreleased structure''' The entry 7ce3 is ON HOLD Authors: Sun, P.K., Ding, J.P. Description: Crystal structure of human IDH3 holoenzyme in APO form. [[Category: Unreleased Structur...

New page

'''Unreleased structure'''

The entry 7ce3 is ON HOLD

Authors: Sun, P.K., Ding, J.P.

Description: Crystal structure of human IDH3 holoenzyme in APO form.
[[Category: Unreleased Structures]]
[[Category: Sun, P.K]]
[[Category: Ding, J.P]]