7ceu - Revision history

OCA at 16:07, 29 November 2023

OCA — Wed, 29 Nov 2023 16:07:09 GMT

←Older revision		Revision as of 16:07, 29 November 2023
Line 3:		Line 3:
	<StructureSection load='7ceu' size='340' side='right'caption='[[7ceu]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='7ceu' size='340' side='right'caption='[[7ceu]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ceu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Campylobacter_pylori Campylobacter~~ pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CEU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ceu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CEU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7TS:(5-HYDROXY-6-METHYL-4-{[(3-OXO-2,3-DIHYDRO-1,2-OXAZOL-4-YL)AMINO]METHYL}PYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>7TS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iscS, HP_0220 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7TS:(5-HYDROXY-6-METHYL-4-{[(3-OXO-2,3-DIHYDRO-1,2-OXAZOL-4-YL)AMINO]METHYL}PYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>7TS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ceu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ceu OCA], [https://pdbe.org/7ceu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ceu RCSB], [https://www.ebi.ac.uk/pdbsum/7ceu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ceu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ceu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ceu OCA], [https://pdbe.org/7ceu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ceu RCSB], [https://www.ebi.ac.uk/pdbsum/7ceu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ceu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ISCS_HELPY ISCS_HELPY]] Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.	+	[https://www.uniprot.org/uniprot/ISCS_HELPY ISCS_HELPY] Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7ceu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7ceu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cysteine desulfurase 3D structures\|Cysteine desulfurase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Campylobacter~~ pylori]]	+	[[Category: Helicobacter pylori 26695]]
-	~~[[Category: Cysteine desulfurase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fujishiro, T]]	+	[[Category: Fujishiro T]]
-	[[Category: Nakamura, R]]	+	[[Category: Nakamura R]]
-	[[Category: Takahashi, Y]]	+	[[Category: Takahashi Y]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Cycloserine]]~~	+
-	~~[[Category: Cysteine metabolism]]~~	+
-	~~[[Category: Fe-s cluster biosynthesis]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Plp-dependent enzyme~~]]	+

OCA at 11:47, 27 April 2022

OCA — Wed, 27 Apr 2022 11:47:20 GMT

←Older revision		Revision as of 11:47, 27 April 2022
Line 1:		Line 1:

	==Crystal structure of L-cycloserine-bound form of cysteine desulfurase NifS from Helicobacter pylori==		==Crystal structure of L-cycloserine-bound form of cysteine desulfurase NifS from Helicobacter pylori==
-	<StructureSection load='7ceu' size='340' side='right'caption='[[7ceu]]' scene=''>	+	<StructureSection load='7ceu' size='340' side='right'caption='[[7ceu]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CEU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ceu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ceu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ceu OCA], [https://pdbe.org/7ceu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ceu RCSB], [https://www.ebi.ac.uk/pdbsum/7ceu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ceu ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7TS:(5-HYDROXY-6-METHYL-4-{[(3-OXO-2,3-DIHYDRO-1,2-OXAZOL-4-YL)AMINO]METHYL}PYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>7TS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iscS, HP_0220 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ceu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ceu OCA], [https://pdbe.org/7ceu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ceu RCSB], [https://www.ebi.ac.uk/pdbsum/7ceu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ceu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ISCS_HELPY ISCS_HELPY]] Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cysteine desulfurase SufS is a pyridoxal-5'-phosphate-dependent enzyme and is essential for the SUF system, which participates in iron-sulfur cluster biosynthesis. Inhibition of SufS in the SUF system by D-cycloserine (DCS) in Plasmodium falciparum apicoplast has recently been reported, indicating that SufS could be a target for malaria therapeutics. However, the mechanistic details underlying the inhibition of SufS by DCS have not yet been clarified. Moreover, inhibition of SufS by the other enantiomer, L-cycloserine (LCS), has not been investigated. Herein, we investigated the structure-based inhibition mechanisms of SufS by DCS and LCS using Bacillus subtilis SufS, whose catalytic mechanism has been well characterized in comparison to that of the P. falciparum SufS. Surprisingly, DCS- and LCS-mediated inhibitions of SufS occur via distinct mechanisms resulting in pyridoxamine-5'-phosphate (PMP) in DCS-mediated inhibition and PMP-3-hydroxyisoxazole adduct (PMP-isoxazole) in LCS-mediated inhibition. Biochemical and structural evaluation of SufS variants identified conserved His and Arg residues at the active site as the key determinants of the distinct inhibition mechanisms. The importance of structural elements involved in DCS and LCS-mediated inhibitions of SufS provides valuable insights for the structure-based design of new drugs targeting SufS. DATABASE: Structural data are available in PDB database under the accession numbers 6KFY, 7CEO, 7CEP, 7CEQ, 7CER, 7CES, 7CET, 7CEU, 7E6A, 7E6B, 7E6C, 7E6D, 7E6E, and 7E6F.
		+
		+	Cycloserine enantiomers inhibit PLP-dependent cysteine desulfurase SufS via distinct mechanisms.,Nakamura R, Ogawa S, Takahashi Y, Fujishiro T FEBS J. 2022 Apr 8. doi: 10.1111/febs.16455. PMID:35395703<ref>PMID:35395703</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ceu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter pylori]]
		+	[[Category: Cysteine desulfurase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fujishiro T]]	+	[[Category: Fujishiro, T]]
-	[[Category: Nakamura R]]	+	[[Category: Nakamura, R]]
-	[[Category: Takahashi Y]]	+	[[Category: Takahashi, Y]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Cycloserine]]
		+	[[Category: Cysteine metabolism]]
		+	[[Category: Fe-s cluster biosynthesis]]
		+	[[Category: Inhibitor]]
		+	[[Category: Plp-dependent enzyme]]

OCA at 03:32, 2 July 2021

OCA — Fri, 02 Jul 2021 03:32:28 GMT

←Older revision		Revision as of 03:32, 2 July 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ceu is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of L-cycloserine-bound form of cysteine desulfurase NifS from Helicobacter pylori==
-		+	<StructureSection load='7ceu' size='340' side='right'caption='[[7ceu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CEU FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ceu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ceu OCA], [https://pdbe.org/7ceu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ceu RCSB], [https://www.ebi.ac.uk/pdbsum/7ceu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ceu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Fujishiro T]]
		+	[[Category: Nakamura R]]
		+	[[Category: Takahashi Y]]

OCA: Protected "7ceu" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 11:28:41 GMT

Protected "7ceu" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:28, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 7ceu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Jul 2020 11:28:40 GMT

New page: '''Unreleased structure''' The entry 7ceu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ceu is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]