7cgk - Revision history

OCA at 16:07, 29 November 2023

OCA — Wed, 29 Nov 2023 16:07:51 GMT

←Older revision		Revision as of 16:07, 29 November 2023
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-	====	+	==Crystal Structure of PUF-8 in Complex with PBE-RNA==
-	<StructureSection load='7cgk' size='340' side='right'caption='[[7cgk]]' scene=''>	+	<StructureSection load='7cgk' size='340' side='right'caption='[[7cgk]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cgk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CGK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cgk OCA], [https://pdbe.org/7cgk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cgk RCSB], [https://www.ebi.ac.uk/pdbsum/7cgk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cgk ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cgk OCA], [https://pdbe.org/7cgk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cgk RCSB], [https://www.ebi.ac.uk/pdbsum/7cgk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cgk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q09487_CAEEL Q09487_CAEEL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The molecular mechanisms of aging are unsolved fundamental biological questions. Caenorhabditis elegans is an ideal model organism for investigating aging. PUF-8, a PUF (Pumilio and FBF) protein in C. elegans, is crucial for germline development through binding with the 3' untranslated regions (3' UTR) in the target mRNAs. Recently, PUF-8 was reported to alter mitochondrial dynamics and mitophagy by regulating MFF-1, a mitochondrial fission factor, and subsequently regulated longevity. Here, we determined the crystal structure of the PUF domain of PUF-8 with an RNA substrate. Mutagenesis experiments were performed to alter PUF-8 recognition of its target mRNAs. Those mutations reduced the fertility and extended the lifespan of C. elegans. Deep sequencing of total mRNAs from wild-type and puf-8 mutant worms as well as in vivo RNA Crosslinking and Immunoprecipitation (CLIP) experiments identified six PUF-8 regulated genes, which contain at least one PUF-binding element (PBE) at the 3' UTR. One of the six genes, pqm-1, is crucial for lipid storage and aging process. Knockdown of pqm-1 could revert the lifespan extension of puf-8 mutant animals. We conclude that PUF-8 regulate the lifespan of C. elegans may not only via MFF but also via modulating pqm-1-related pathways.
		+
		+	Structural recognition of the mRNA 3' UTR by PUF-8 restricts the lifespan of C. elegans.,Xu Z, Zhao J, Hong M, Zeng C, Guang S, Shi Y Nucleic Acids Res. 2021 Sep 27;49(17):10082-10097. doi: 10.1093/nar/gkab754. PMID:34478557<ref>PMID:34478557</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cgk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Shouhong G]]
		+	[[Category: Yunyu S]]
		+	[[Category: Zheng X]]

OCA at 09:56, 7 July 2021

OCA — Wed, 07 Jul 2021 09:56:10 GMT

←Older revision		Revision as of 09:56, 7 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7cgk ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7cgk' size='340' side='right'caption='[[7cgk]]' scene=''>
-	~~Authors~~: ~~Zheng, X~~.~~, Yunyu, S~~.~~, Shouhong, G~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal Structure of PUF-8 in Complex with PBE~~-~~RNA~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cgk OCA], [https://pdbe.org/7cgk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cgk RCSB], [https://www.ebi.ac.uk/pdbsum/7cgk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cgk ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Shouhong~~, G]]	+	__TOC__
-	[[Category: ~~Zheng, X~~]]	+	</StructureSection>
-	[[Category: ~~Yunyu, S~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:27, 9 September 2020

OCA — Wed, 09 Sep 2020 07:27:12 GMT

←Older revision		Revision as of 07:27, 9 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cgk is ON HOLD	+	The entry 7cgk is ON HOLD until Paper Publication

	Authors: Zheng, X., Yunyu, S., Shouhong, G.		Authors: Zheng, X., Yunyu, S., Shouhong, G.
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	Description: Crystal Structure of PUF-8 in Complex with PBE-RNA		Description: Crystal Structure of PUF-8 in Complex with PBE-RNA
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zheng, X]]
	[[Category: Shouhong, G]]		[[Category: Shouhong, G]]
		+	[[Category: Zheng, X]]
	[[Category: Yunyu, S]]		[[Category: Yunyu, S]]

OCA: Protected "7cgk" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 11:33:07 GMT

Protected "7cgk" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:33, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 7cgk is ON HOLD Authors: Zheng, X., Yunyu, S., Shouhong, G. Description: Crystal Structure of PUF-8 in Complex with PBE-RNA [[Category: Unreleased ...

OCA — Wed, 08 Jul 2020 11:33:06 GMT

New page: '''Unreleased structure''' The entry 7cgk is ON HOLD Authors: Zheng, X., Yunyu, S., Shouhong, G. Description: Crystal Structure of PUF-8 in Complex with PBE-RNA [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 7cgk is ON HOLD

Authors: Zheng, X., Yunyu, S., Shouhong, G.

Description: Crystal Structure of PUF-8 in Complex with PBE-RNA
[[Category: Unreleased Structures]]
[[Category: Zheng, X]]
[[Category: Shouhong, G]]
[[Category: Yunyu, S]]