7chy - Revision history

OCA at 16:08, 29 November 2023

OCA — Wed, 29 Nov 2023 16:08:26 GMT

←Older revision		Revision as of 16:08, 29 November 2023
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-	====	+	==Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragment Of IgG26==
-	<StructureSection load='7chy' size='340' side='right'caption='[[7chy]]' scene=''>	+	<StructureSection load='7chy' size='340' side='right'caption='[[7chy]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7chy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CHY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7chy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7chy OCA], [https://pdbe.org/7chy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7chy RCSB], [https://www.ebi.ac.uk/pdbsum/7chy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7chy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7chy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7chy OCA], [https://pdbe.org/7chy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7chy RCSB], [https://www.ebi.ac.uk/pdbsum/7chy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7chy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Interleukin-1beta (IL-1beta) is a potent pleiotropic cytokine playing a central role in protecting cells from microbial pathogen infection or endogenous stress. After it binds to IL-1RI and recruits IL-1 receptor accessory protein (IL-1RAcP), signaling culminates in activation of NF-kappaB. Many pathophysiological diseases have been attributed to the derailment of IL-1beta regulation. Several blocking reagents have been developed based on two mechanisms: blocking the binding of IL-1beta to IL-1RI or inhibiting the recruitment of IL-1RAcP to the IL-1beta initial complex. In order to simultaneously fulfill these two actions, a human anti-IL-1beta neutralizing antibody IgG26 was screened from human genetic phage-display library and furthered structure-optimized to final version, IgG26AW. IgG26AW has a sub-nanomolar binding affinity for human IL-1beta. We validated IgG26AW-neutralizing antibodies specific for IL-1beta in vivo to prevent human IL-1beta-driving IL-6 elevation in C56BL/6 mice. Mice underwent treatments with IgG26AW in A549 and MDA-MB-231 xenograft mouse cancer models have also been observed with tumor shrank and inhibition of tumor metastasis. The region where IgG26 binds to IL-1beta also overlaps with the position where IL-1RI and IL-1RAcP bind, as revealed by the 26-Fab/IL-1beta complex structure. Meanwhile, SPR experiments showed that IL-1beta bound by IgG26AW prevented the further binding of IL-1RI and IL-1RAcP, which confirmed our inference from the result of protein structure. Therefore, the inhibitory mechanism of IgG26AW is to block the assembly of the IL-1beta/IL-1RI/IL-1RAcP ternary complex which further inhibits downstream signaling. Based on its high affinity, high neutralizing potency, and novel binding epitope simultaneously occupying both IL-1RI and IL-1RAcP residues that bind to IL-1beta, IgG26AW may be a new candidate for treatments of inflammation-related diseases or for complementary treatments of cancers in which the role of IL-1beta is critical to pathogenesis.
		+
		+	Structure-based Development of Human Interleukin-1beta-Specific Antibody That Simultaneously Inhibits Binding to Both IL-1RI and IL-1RAcP.,Kuo WC, Lee CC, Chang YW, Pang W, Chen HS, Hou SC, Lo SY, Yang AS, Wang AH J Mol Biol. 2021 Feb 19;433(4):166766. doi: 10.1016/j.jmb.2020.166766. Epub 2020 , Dec 24. PMID:33359099<ref>PMID:33359099</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7chy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Interleukin 3D structures\|Interleukin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Kuo WC]]
		+	[[Category: Lee CC]]
		+	[[Category: Wang AHJ]]

OCA at 08:02, 27 January 2021

OCA — Wed, 27 Jan 2021 08:02:37 GMT

←Older revision		Revision as of 08:02, 27 January 2021
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	<StructureSection load='7chy' size='340' side='right'caption='[[7chy]]' scene=''>		<StructureSection load='7chy' size='340' side='right'caption='[[7chy]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7chy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7chy OCA], [~~http~~://pdbe.org/7chy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7chy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7chy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7chy ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7chy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7chy OCA], [https://pdbe.org/7chy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7chy RCSB], [https://www.ebi.ac.uk/pdbsum/7chy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7chy ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 05:54, 20 January 2021

OCA — Wed, 20 Jan 2021 05:54:36 GMT

←Older revision		Revision as of 05:54, 20 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7chy ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7chy' size='340' side='right'caption='[[7chy]]' scene=''>
-	~~Authors~~: ~~Lee, C~~.C.~~, Wang, A~~.H.J.~~, Kuo, W~~.C.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragment Of IgG26~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7chy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7chy OCA], [http://pdbe.org/7chy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7chy RCSB], [http://www.ebi.ac.uk/pdbsum/7chy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7chy ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Kuo~~, W.C]]	+	__TOC__
-	[[Category: ~~Wang, A.H.J~~]]	+	</StructureSection>
-	[[Category: ~~Lee, C.C~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:40, 29 July 2020

OCA — Wed, 29 Jul 2020 09:40:15 GMT

←Older revision		Revision as of 09:40, 29 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7chy is ON HOLD	+	The entry 7chy is ON HOLD until Paper Publication

	Authors: Lee, C.C., Wang, A.H.J., Kuo, W.C.		Authors: Lee, C.C., Wang, A.H.J., Kuo, W.C.
Line 7:		Line 7:
	Description: Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragment Of IgG26		Description: Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragment Of IgG26
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Lee, C.C]]
	[[Category: Kuo, W.C]]		[[Category: Kuo, W.C]]
	[[Category: Wang, A.H.J]]		[[Category: Wang, A.H.J]]
		+	[[Category: Lee, C.C]]

OCA: Protected "7chy" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 08:30:02 GMT

Protected "7chy" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:30, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 7chy is ON HOLD Authors: Lee, C.C., Wang, A.H.J., Kuo, W.C. Description: Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragm...

OCA — Wed, 22 Jul 2020 08:30:00 GMT

New page: '''Unreleased structure''' The entry 7chy is ON HOLD Authors: Lee, C.C., Wang, A.H.J., Kuo, W.C. Description: Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragm...

New page

'''Unreleased structure'''

The entry 7chy is ON HOLD

Authors: Lee, C.C., Wang, A.H.J., Kuo, W.C.

Description: Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragment Of IgG26
[[Category: Unreleased Structures]]
[[Category: Lee, C.C]]
[[Category: Kuo, W.C]]
[[Category: Wang, A.H.J]]