7ciy - Revision history

OCA at 16:09, 29 November 2023

OCA — Wed, 29 Nov 2023 16:09:13 GMT

←Older revision		Revision as of 16:09, 29 November 2023
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-	====	+	==Crystal structure of N191G-mutated tyrosinase from Streptomyces castaneoglobisporus in complex with the caddie protein obtained by soaking in the solution containing Cu(II) and hydroxylamine for 24 h==
-	<StructureSection load='7ciy' size='340' side='right'caption='[[7ciy]]' scene=''>	+	<StructureSection load='7ciy' size='340' side='right'caption='[[7ciy]], [[Resolution\|resolution]] 1.47Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ciy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_castaneoglobisporus Streptomyces castaneoglobisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CIY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ciy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ciy OCA], [https://pdbe.org/7ciy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ciy RCSB], [https://www.ebi.ac.uk/pdbsum/7ciy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ciy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.47Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=G1X:(2S)-2-azanyl-3-[3,4-bis(oxidanylidene)cyclohexa-1,5-dien-1-yl]propanoic+acid'>G1X</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PEO:HYDROGEN+PEROXIDE'>PEO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ciy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ciy OCA], [https://pdbe.org/7ciy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ciy RCSB], [https://www.ebi.ac.uk/pdbsum/7ciy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ciy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q83WS2_9ACTN Q83WS2_9ACTN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tyrosinase (Ty) and catechol oxidase (CO) are members of type-3 copper enzymes. While Ty catalyzes both phenolase and catecholase reactions, CO catalyzes only the latter reaction. In the present study, Ty was found to catalyze the catecholase reaction, but hardly the phenolase reaction in the presence of the metallochaperon called "caddie protein (Cad)". The ability of the substrates to dissociate the motif shielding the active-site pocket seems to contribute critically to the substrate specificity of Ty. In addition, a mutation at the N191 residue, which forms a hydrogen bond with a water molecule near the active center, decreased the inherent ratio of phenolase versus catecholase activity. Unlike the wild-type complex, reaction intermediates were not observed when the catalytic reaction toward the Y98 residue of Cad was progressed in the crystalline state. The increased basicity of the water molecule may be necessary to inhibit the proton transfer from the conjugate acid to a hydroxide ion bridging the two copper ions. The deprotonation of the substrate hydroxyl by the bridging hydroxide seems to be significant for the efficient catalytic cycle of the phenolase reaction.
		+
		+	The basicity of an active-site water molecule discriminates between tyrosinase and catechol oxidase activity.,Matoba Y, Oda K, Muraki Y, Masuda T Int J Biol Macromol. 2021 Jul 31;183:1861-1870. doi: , 10.1016/j.ijbiomac.2021.05.206. Epub 2021 Jun 2. PMID:34089758<ref>PMID:34089758</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ciy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tyrosinase 3D structures\|Tyrosinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Streptomyces castaneoglobisporus]]
		+	[[Category: Matoba Y]]
		+	[[Category: Oda K]]

OCA at 14:45, 17 June 2021

OCA — Thu, 17 Jun 2021 14:45:46 GMT

←Older revision		Revision as of 14:45, 17 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7ciy ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ciy' size='340' side='right'caption='[[7ciy]]' scene=''>
-	~~Authors~~: ~~Oda, K~~.~~, Matoba, Y~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of N191G~~-~~mutated tyrosinase from Streptomyces castaneoglobisporus in complex with~~ the ~~caddie protein obtained by soaking in the solution containing Cu(II) and hydroxylamine for 24 h~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ciy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ciy OCA], [https://pdbe.org/7ciy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ciy RCSB], [https://www.ebi.ac.uk/pdbsum/7ciy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ciy ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Oda, K~~]]	+	__TOC__
-	[[Category: ~~Matoba, Y~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:28, 9 September 2020

OCA — Wed, 09 Sep 2020 07:28:00 GMT

←Older revision		Revision as of 07:28, 9 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ciy is ON HOLD	+	The entry 7ciy is ON HOLD until Paper Publication

	Authors: Oda, K., Matoba, Y.		Authors: Oda, K., Matoba, Y.

OCA: Protected "7ciy" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 08:33:24 GMT

Protected "7ciy" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:33, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 7ciy is ON HOLD Authors: Oda, K., Matoba, Y. Description: Crystal structure of N191G-mutated tyrosinase from Streptomyces castaneoglobisporus in co...

OCA — Wed, 22 Jul 2020 08:33:22 GMT

New page: '''Unreleased structure''' The entry 7ciy is ON HOLD Authors: Oda, K., Matoba, Y. Description: Crystal structure of N191G-mutated tyrosinase from Streptomyces castaneoglobisporus in co...

New page

'''Unreleased structure'''

The entry 7ciy is ON HOLD

Authors: Oda, K., Matoba, Y.

Description: Crystal structure of N191G-mutated tyrosinase from Streptomyces castaneoglobisporus in complex with the caddie protein obtained by soaking in the solution containing Cu(II) and hydroxylamine for 24 h
[[Category: Unreleased Structures]]
[[Category: Oda, K]]
[[Category: Matoba, Y]]