OCA at 16:11, 29 November 2023

OCA — Wed, 29 Nov 2023 16:11:12 GMT

←Older revision		Revision as of 16:11, 29 November 2023
Line 1:		Line 1:

-	====	+	==Crystal Structure of BRCT7/8 in Complex with the APS Motif of PHF8==
-	<StructureSection load='7cmz' size='340' side='right'caption='[[7cmz]]' scene=''>	+	<StructureSection load='7cmz' size='340' side='right'caption='[[7cmz]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cmz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CMZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cmz OCA], [https://pdbe.org/7cmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cmz RCSB], [https://www.ebi.ac.uk/pdbsum/7cmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cmz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.695Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cmz OCA], [https://pdbe.org/7cmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cmz RCSB], [https://www.ebi.ac.uk/pdbsum/7cmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cmz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TOPB1_HUMAN TOPB1_HUMAN] Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.<ref>PMID:10498869</ref> <ref>PMID:11395493</ref> <ref>PMID:11714696</ref> <ref>PMID:12697828</ref> <ref>PMID:15075294</ref> <ref>PMID:16530042</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The checkpoint kinase ATR [ATM (ataxia-telangiectasia mutated) and rad3-related] is a master regulator of DNA damage response. Yet, how ATR activity is regulated remains to be investigated. We report here that histone demethylase PHF8 (plant homeodomain finger protein 8) plays a key role in ATR activation and replication stress response. Mechanistically, PHF8 interacts with and demethylates TOPBP1 (DNA topoisomerase 2-binding protein 1), an essential allosteric activator of ATR, under unperturbed conditions, but replication stress results in PHF8 phosphorylation and dissociation from TOPBP1. Consequently, hypomethylated TOPBP1 facilitates RAD9 (RADiation sensitive 9) binding and chromatin loading of the TOPBP1-RAD9 complex to fully activate ATR and thus safeguard the genome and protect cells against replication stress. Our study uncovers a demethylation and phosphorylation code that controls the assembly of TOPBP1-scaffolded protein complex, and provides molecular insight into non-histone methylation switch in ATR activation.
		+
		+	PHF8-promoted TOPBP1 demethylation drives ATR activation and preserves genome stability.,Ma S, Cao C, Che S, Wang Y, Su D, Liu S, Gong W, Liu L, Sun J, Zhao J, Wang Q, Song N, Ge T, Guo Q, Tian S, Chen CD, Zhang T, Wang J, Ding X, Yang F, Ying G, Yang J, Zhang K, Zhu Y, Yao Z, Yang N, Shi L Sci Adv. 2021 May 5;7(19):eabf7684. doi: 10.1126/sciadv.abf7684. Print 2021 May. PMID:33952527<ref>PMID:33952527</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cmz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Topoisomerase binding protein\|Topoisomerase binding protein]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Cao C]]
		+	[[Category: Che SY]]
		+	[[Category: Ma S]]
		+	[[Category: Shi L]]
		+	[[Category: Yang N]]
		+	[[Category: Yao Z]]

OCA at 06:46, 17 March 2021

OCA — Wed, 17 Mar 2021 06:46:26 GMT

←Older revision		Revision as of 06:46, 17 March 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7cmz ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7cmz' size='340' side='right'caption='[[7cmz]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cmz OCA], [https://pdbe.org/7cmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cmz RCSB], [https://www.ebi.ac.uk/pdbsum/7cmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cmz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:21, 12 August 2020

OCA — Wed, 12 Aug 2020 08:21:06 GMT

←Older revision		Revision as of 08:21, 12 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cmz is ON HOLD	+	The entry 7cmz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7cmz" [edit=sysop:move=sysop]

OCA — Wed, 05 Aug 2020 04:11:37 GMT

Protected "7cmz" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 5 August 2020

OCA: New page: '''Unreleased structure''' The entry 7cmz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Aug 2020 04:11:36 GMT

New page: '''Unreleased structure''' The entry 7cmz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7cmz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7cmz - Revision history

OCA at 16:11, 29 November 2023

OCA at 06:46, 17 March 2021

OCA at 08:21, 12 August 2020

OCA: Protected "7cmz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7cmz is ON HOLD Authors: Description: Category: Unreleased Structures