OCA at 10:55, 15 November 2023

OCA — Wed, 15 Nov 2023 10:55:05 GMT

←Older revision		Revision as of 10:55, 15 November 2023
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-	====	+	==Crystal structure of 8PE bound PSD from E. coli (2.12 A)==
-	<StructureSection load='7cny' size='340' side='right'caption='[[7cny]]' scene=''>	+	<StructureSection load='7cny' size='340' side='right'caption='[[7cny]], [[Resolution\|resolution]] 2.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cny]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CNY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cny OCA], [https://pdbe.org/7cny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cny RCSB], [https://www.ebi.ac.uk/pdbsum/7cny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cny ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.12Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G8C:1,2-Dioctanoyl-SN-Glycero-3-Phosphoethanolamine'>G8C</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cny OCA], [https://pdbe.org/7cny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cny RCSB], [https://www.ebi.ac.uk/pdbsum/7cny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cny ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PSD_ECOLI PSD_ECOLI] Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Only decarboxylates the lipid-linked form of the serine moiety, and not serine alone or derivatives like phosphoserine or glycerophosphoserine.[HAMAP-Rule:MF_00662]<ref>PMID:4598120</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phosphatidylethanolamine (PE), a major component of the cellular membrane across all domains of life, is synthesized exclusively by membrane-anchored phosphatidylserine decarboxylase (PSD) in most bacteria. The enzyme undergoes auto-cleavage for activation and utilizes the pyruvoyl moiety to form a Schiff base intermediate with PS to facilitate decarboxylation. However, the structural basis for self-maturation, PS binding, and decarboxylation processes directed by PSD remain unclear. Here, we present X-ray crystal structures of PSD from Escherichia coli, representing an apo form and a PE-bound complex, in which the phospholipid is chemically conjugated to the essential pyruvoyl residue, mimicking the Schiff base intermediate. The high-resolution structures of PE-complexed PSD clearly illustrate extensive hydrophobic interactions with the fatty acyl chains of the phospholipid, providing insights into the broad specificity of the enzyme over a wide range of cellular PS. Furthermore, these structures strongly advocate the unique topology of the enzyme in a lipid bilayer environment, where the enzyme associates with cell membranes in a monotopic fashion via the N-terminal domain composed of three amphipathic helices. Lastly, mutagenesis analyses reveal that E. coli PSD primarily employs D90/D142-H144-S254 to achieve auto-cleavage for the proenzyme maturation, where D90 and D142 act in complementary to each other.
		+
		+	Structural insights into phosphatidylethanolamine formation in bacterial membrane biogenesis.,Cho G, Lee E, Kim J Sci Rep. 2021 Mar 11;11(1):5785. doi: 10.1038/s41598-021-85195-5. PMID:33707636<ref>PMID:33707636</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cny" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Cho G]]
		+	[[Category: Kim J]]

OCA at 06:40, 24 March 2021

OCA — Wed, 24 Mar 2021 06:40:23 GMT

←Older revision		Revision as of 06:40, 24 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7cny ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7cny' size='340' side='right'caption='[[7cny]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cny OCA], [https://pdbe.org/7cny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cny RCSB], [https://www.ebi.ac.uk/pdbsum/7cny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cny ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:28, 24 February 2021

OCA — Wed, 24 Feb 2021 05:28:00 GMT

←Older revision		Revision as of 05:28, 24 February 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cny is ON HOLD	+	The entry 7cny is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7cny" [edit=sysop:move=sysop]

OCA — Wed, 12 Aug 2020 08:22:13 GMT

Protected "7cny" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:22, 12 August 2020

OCA: New page: '''Unreleased structure''' The entry 7cny is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Aug 2020 08:22:12 GMT

New page: '''Unreleased structure''' The entry 7cny is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7cny is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7cny - Revision history

OCA at 10:55, 15 November 2023

OCA at 06:40, 24 March 2021

OCA at 05:28, 24 February 2021

OCA: Protected "7cny" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7cny is ON HOLD Authors: Description: Category: Unreleased Structures