OCA at 16:12, 29 November 2023

OCA — Wed, 29 Nov 2023 16:12:02 GMT

←Older revision		Revision as of 16:12, 29 November 2023
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-	====	+	==Ternary complex of DNA polymerase Mu with 1-nt gapped DNA (T:dGMPNPP) and Mn==
-	<StructureSection load='7coa' size='340' side='right'caption='[[7coa]]' scene=''>	+	<StructureSection load='7coa' size='340' side='right'caption='[[7coa]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7coa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7COA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7COA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7coa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7coa OCA], [https://pdbe.org/7coa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7coa RCSB], [https://www.ebi.ac.uk/pdbsum/7coa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7coa ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.698Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XG4:2-DEOXY-5-O-[(R)-HYDROXY{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]AMINO}PHOSPHORYL]GUANOSINE'>XG4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7coa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7coa OCA], [https://pdbe.org/7coa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7coa RCSB], [https://www.ebi.ac.uk/pdbsum/7coa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7coa ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DPOLM_HUMAN DPOLM_HUMAN] Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.<ref>PMID:12640116</ref> <ref>PMID:12888504</ref> <ref>PMID:17483519</ref> <ref>PMID:17915942</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pol mu is capable of performing gap-filling repair synthesis in the nonhomologous end joining (NHEJ) pathway. Together with DNA ligase, misincorporation of dGTP opposite the templating T by Pol mu results in a promutagenic T:G mispair, leading to genomic instability. Here, crystal structures and kinetics of Pol mu substituting dGTP for dATP on gapped DNA substrates containing templating T were determined and compared. Pol mu is highly mutagenic on a 2-nt gapped DNA substrate, with T:dGTP base pairing at the 3' end of the gap. Two residues (Lys438 and Gln441) interact with T:dGTP and fine tune the active site microenvironments. The in-crystal misincorporation reaction of Pol mu revealed an unexpected second dGTP in the active site, suggesting its potential mutagenic role among human X family polymerases in NHEJ.
		+
		+	Mechanism of genome instability mediated by human DNA polymerase mu misincorporation.,Guo M, Wang Y, Tang Y, Chen Z, Hou J, Dai J, Wang Y, Wang L, Xu H, Tian B, Hua Y, Zhao Y Nat Commun. 2021 Jun 18;12(1):3759. doi: 10.1038/s41467-021-24096-7. PMID:34145298<ref>PMID:34145298</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7coa" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Guo M]]
		+	[[Category: Zhao Y]]

OCA at 03:32, 2 July 2021

OCA — Fri, 02 Jul 2021 03:32:45 GMT

←Older revision		Revision as of 03:32, 2 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7coa ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7coa' size='340' side='right'caption='[[7coa]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7coa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7coa OCA], [https://pdbe.org/7coa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7coa RCSB], [https://www.ebi.ac.uk/pdbsum/7coa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7coa ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:10, 2 September 2020

OCA — Wed, 02 Sep 2020 05:10:07 GMT

←Older revision		Revision as of 05:10, 2 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7coa is ON HOLD	+	The entry 7coa is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7coa" [edit=sysop:move=sysop]

OCA — Wed, 12 Aug 2020 08:22:52 GMT

Protected "7coa" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:22, 12 August 2020

OCA: New page: '''Unreleased structure''' The entry 7coa is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Aug 2020 08:22:51 GMT

New page: '''Unreleased structure''' The entry 7coa is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7coa is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7coa - Revision history

OCA at 16:12, 29 November 2023

OCA at 03:32, 2 July 2021

OCA at 05:10, 2 September 2020

OCA: Protected "7coa" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7coa is ON HOLD Authors: Description: Category: Unreleased Structures