7cq1 - Revision history

OCA at 11:12, 14 June 2023

OCA — Wed, 14 Jun 2023 11:12:15 GMT

←Older revision		Revision as of 11:12, 14 June 2023
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	==Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM==		==Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM==
-	<StructureSection load='7cq1' size='340' side='right'caption='[[7cq1~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='7cq1' size='340' side='right'caption='[[7cq1]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7cq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQ1 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rimM, Rv2907c, MTCY274.38c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cq1 OCA], [https://pdbe.org/7cq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7cq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cq1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cq1 OCA], [https://pdbe.org/7cq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7cq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cq1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RIMM_MYCTU RIMM_MYCTU]] An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.	+	[https://www.uniprot.org/uniprot/RIMM_MYCTU RIMM_MYCTU] An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Lin, D]]	+	[[Category: Lin D]]
-	[[Category: Zhang, H]]	+	[[Category: Zhang H]]
-	~~[[Category: 30s subunit]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Ribosome maturation~~]]	+

OCA at 10:24, 19 May 2021

OCA — Wed, 19 May 2021 10:24:01 GMT

←Older revision		Revision as of 10:24, 19 May 2021
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RIMM_MYCTU RIMM_MYCTU]] An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.		[[https://www.uniprot.org/uniprot/RIMM_MYCTU RIMM_MYCTU]] An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multidrug-resistant tuberculosis (TB) is a serious threat to public health, calling for the development of new anti-TB drugs. Chaperon protein RimM, involved in the assembly of ribosomal protein S19 into 30S ribosomal subunit during ribosome maturation, is a potential drug target for TB treatment. The C-terminal domain (CTD) of RimM is primarily responsible for binding S19. However, both the CTD structure of RimM from Mycobacterium tuberculosis (MtbRimMCTD) and the molecular mechanisms underlying MtbRimMCTD binding S19 remain elusive. Here, we report the solution structure, dynamics features of MtbRimMCTD, and its interaction with S19. MtbRimMCTD has a rigid hydrophobic core comprised of a relatively conservative six-strand beta-barrel, tailed with a short alpha-helix and interspersed with flexible loops. Using several biophysical techniques including surface plasmon resonance (SPR) affinity assays, nuclear magnetic resonance (NMR) assays, and molecular docking, we established a structural model of the MtbRimMCTD-S19 complex and indicated that the beta4-beta5 loop and two nonconserved key residues (D105 and H129) significantly contributed to the unique pattern of MtbRimMCTD binding S19, which might be implicated in a form of orthogonality for species-dependent RimM-S19 interaction. Our study provides the structural basis for MtbRimMCTD binding S19 and is beneficial to the further exploration of MtbRimM as a potential target for the development of new anti-TB drugs.
		+
		+	Structural Basis for the C-Terminal Domain of Mycobacterium tuberculosis Ribosome Maturation Factor RimM to Bind Ribosomal Protein S19.,Zhang H, Zhou Q, Guo C, Feng L, Wang H, Liao X, Lin D Biomolecules. 2021 Apr 18;11(4). pii: biom11040597. doi: 10.3390/biom11040597. PMID:33919647<ref>PMID:33919647</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cq1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:32, 28 April 2021

OCA — Wed, 28 Apr 2021 05:32:09 GMT

←Older revision		Revision as of 05:32, 28 April 2021
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	==Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM==		==Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM==
-	<StructureSection load='7cq1' size='340' side='right'caption='[[7cq1]]' scene=''>	+	<StructureSection load='7cq1' size='340' side='right'caption='[[7cq1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQ1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cq1 OCA], [https://pdbe.org/7cq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7cq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cq1 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rimM, Rv2907c, MTCY274.38c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cq1 OCA], [https://pdbe.org/7cq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7cq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cq1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/RIMM_MYCTU RIMM_MYCTU]] An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lin D]]	+	[[Category: Myctu]]
-	[[Category: Zhang H]]	+	[[Category: Lin, D]]
		+	[[Category: Zhang, H]]
		+	[[Category: 30s subunit]]
		+	[[Category: Protein binding]]
		+	[[Category: Ribosome maturation]]

OCA at 10:31, 31 March 2021

OCA — Wed, 31 Mar 2021 10:31:45 GMT

←Older revision		Revision as of 10:31, 31 March 2021
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-	'''Unreleased structure'''

-	~~The entry 7cq1 is ON HOLD until Paper Publication~~	+	==Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM==
-		+	<StructureSection load='7cq1' size='340' side='right'caption='[[7cq1]]' scene=''>
-	~~Authors: Zhang, H., Lin, D.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQ1 FirstGlance]. <br>
-	~~Description:~~ Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cq1 OCA], [https://pdbe.org/7cq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7cq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cq1 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Zhang~~, H]]	+	__TOC__
-	[[Category: Lin, D]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lin D]]
		+	[[Category: Zhang H]]

OCA at 05:10, 2 September 2020

OCA — Wed, 02 Sep 2020 05:10:31 GMT

←Older revision		Revision as of 05:10, 2 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cq1 is ON HOLD	+	The entry 7cq1 is ON HOLD until Paper Publication

	Authors: Zhang, H., Lin, D.		Authors: Zhang, H., Lin, D.

-	Description: C-terminal domain of Mycobacterium Tuberculosis RimM	+	Description: Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Zhang, H]]		[[Category: Zhang, H]]
	[[Category: Lin, D]]		[[Category: Lin, D]]

OCA: Protected "7cq1" [edit=sysop:move=sysop]

OCA — Wed, 19 Aug 2020 04:43:27 GMT

Protected "7cq1" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:43, 19 August 2020

OCA: New page: '''Unreleased structure''' The entry 7cq1 is ON HOLD Authors: Zhang, H., Lin, D. Description: C-terminal domain of Mycobacterium Tuberculosis RimM Category: Unreleased Structures [...

OCA — Wed, 19 Aug 2020 04:43:26 GMT

New page: '''Unreleased structure''' The entry 7cq1 is ON HOLD Authors: Zhang, H., Lin, D. Description: C-terminal domain of Mycobacterium Tuberculosis RimM Category: Unreleased Structures [...

New page

'''Unreleased structure'''

The entry 7cq1 is ON HOLD

Authors: Zhang, H., Lin, D.

Description: C-terminal domain of Mycobacterium Tuberculosis RimM
[[Category: Unreleased Structures]]
[[Category: Zhang, H]]
[[Category: Lin, D]]