7cqy - Revision history

OCA at 16:14, 29 November 2023

OCA — Wed, 29 Nov 2023 16:14:08 GMT

←Older revision		Revision as of 16:14, 29 November 2023
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	==Tetrathionate hydrolase from Acidithiobacillus ferrooxidans mutant - D325N==		==Tetrathionate hydrolase from Acidithiobacillus ferrooxidans mutant - D325N==
-	<StructureSection load='7cqy' size='340' side='right'caption='[[7cqy]]' scene=''>	+	<StructureSection load='7cqy' size='340' side='right'caption='[[7cqy]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cqy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cqy OCA], [https://pdbe.org/7cqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cqy RCSB], [https://www.ebi.ac.uk/pdbsum/7cqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cqy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8003578Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cqy OCA], [https://pdbe.org/7cqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cqy RCSB], [https://www.ebi.ac.uk/pdbsum/7cqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cqy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TTH_ACIF2 TTH_ACIF2] Catalyzes the hydrolysis of tetrathionate to generate elemental sulfur, thiosulfate and sulfate.<ref>PMID:17904676</ref> <ref>PMID:25144400</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tetrathionate hydrolase (4THase) plays an important role in dissimilatory sulfur oxidation in the acidophilic iron- and sulfur-oxidizing bacterium Acidithiobacillus ferrooxidans. The structure of recombinant 4THase from A. ferrooxidans (Af-Tth) was determined by X-ray crystallography to a resolution of 1.95 A. Af-Tth is a homodimer, and its monomer structure exhibits an eight-bladed beta-propeller motif. Two insertion loops participate in dimerization, and one loop forms a cavity with the beta-propeller region. We observed unexplained electron densities in this cavity of the substrate-soaked structure. The anomalous difference map generated using diffraction data collected at a wavelength of 1.9 A indicated the presence of polymerized sulfur atoms. Asp325, a highly conserved residue among 4THases, was located near the polymerized sulfur atoms. 4THase activity was completely abolished in the site-specific Af-Tth D325N variant, suggesting that Asp325 plays a crucial role in the first step of tetrathionate hydrolysis. Considering that the Af-Tth reaction occurs only under acidic pH, Asp325 acts as an acid for the tetrathionate hydrolysis reaction. The polymerized sulfur atoms in the active site cavity may represent the intermediate product in the subsequent step.
		+
		+	Reaction mechanism of tetrathionate hydrolysis based on the crystal structure of tetrathionate hydrolase from Acidithiobacillus ferrooxidans.,Kanao T, Hase N, Nakayama H, Yoshida K, Nishiura K, Kosaka M, Kamimura K, Hirano Y, Tamada T Protein Sci. 2020 Oct 25. doi: 10.1002/pro.3984. PMID:33103311<ref>PMID:33103311</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7cqy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acidithiobacillus ferrooxidans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hirano Y]]		[[Category: Hirano Y]]
	[[Category: Tamada T]]		[[Category: Tamada T]]

OCA at 07:57, 27 January 2021

OCA — Wed, 27 Jan 2021 07:57:26 GMT

←Older revision		Revision as of 07:57, 27 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7cqy ~~is ON HOLD until Paper Publication~~	+	==Tetrathionate hydrolase from Acidithiobacillus ferrooxidans mutant - D325N==
-		+	<StructureSection load='7cqy' size='340' side='right'caption='[[7cqy]]' scene=''>
-	~~Authors~~: ~~Tamada~~, T., ~~Hirano~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CQY FirstGlance]. <br>
-	~~Description~~: ~~Tetrathionate hydrolase from Acidithiobacillus ferrooxidans mutant~~ -~~D325N~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cqy OCA], [https://pdbe.org/7cqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cqy RCSB], [https://www.ebi.ac.uk/pdbsum/7cqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cqy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Hirano, Y]]	+	__TOC__
-	[[Category: Tamada, T]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hirano Y]]
		+	[[Category: Tamada T]]

OCA at 09:23, 26 August 2020

OCA — Wed, 26 Aug 2020 09:23:29 GMT

←Older revision		Revision as of 09:23, 26 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cqy is ON HOLD	+	The entry 7cqy is ON HOLD until Paper Publication

	Authors: Tamada, T., Hirano, Y.		Authors: Tamada, T., Hirano, Y.

OCA: Protected "7cqy" [edit=sysop:move=sysop]

OCA — Wed, 19 Aug 2020 04:45:43 GMT

Protected "7cqy" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:45, 19 August 2020

OCA: New page: '''Unreleased structure''' The entry 7cqy is ON HOLD Authors: Tamada, T., Hirano, Y. Description: Tetrathionate hydrolase from Acidithiobacillus ferrooxidans mutant -D325N [[Category: ...

OCA — Wed, 19 Aug 2020 04:45:41 GMT

New page: '''Unreleased structure''' The entry 7cqy is ON HOLD Authors: Tamada, T., Hirano, Y. Description: Tetrathionate hydrolase from Acidithiobacillus ferrooxidans mutant -D325N [[Category: ...

New page

'''Unreleased structure'''

The entry 7cqy is ON HOLD

Authors: Tamada, T., Hirano, Y.

Description: Tetrathionate hydrolase from Acidithiobacillus ferrooxidans mutant -D325N
[[Category: Unreleased Structures]]
[[Category: Hirano, Y]]
[[Category: Tamada, T]]