OCA at 16:14, 29 November 2023

OCA — Wed, 29 Nov 2023 16:14:27 GMT

←Older revision		Revision as of 16:14, 29 November 2023
Line 1:		Line 1:

	==Aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis-APO Structure==		==Aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis-APO Structure==
-	<StructureSection load='7crm' size='340' side='right'caption='[[7crm]]' scene=''>	+	<StructureSection load='7crm' size='340' side='right'caption='[[7crm]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CRM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7crm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CRM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7crm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7crm OCA], [https://pdbe.org/7crm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7crm RCSB], [https://www.ebi.ac.uk/pdbsum/7crm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7crm ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.487Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7crm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7crm OCA], [https://pdbe.org/7crm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7crm RCSB], [https://www.ebi.ac.uk/pdbsum/7crm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7crm ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AAC2_MYCS2 AAC2_MYCS2] Catalyzes the coenzyme A-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides. It confers resistance to aminoglycosides.<ref>PMID:9159528</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The expression of aminoglycoside-modifying enzymes represents a survival strategy of antibiotic-resistant bacteria. Aminoglycoside 2'-N-acetyltransferase [AAC(2')] neutralizes aminoglycoside drugs by acetylation of their 2' amino groups in an acetyl coenzyme A (CoA)-dependent manner. To understand the structural features and molecular mechanism underlying AAC(2') activity, we overexpressed, purified, and crystallized AAC(2') from Mycolicibacterium smegmatis [AAC(2')-Id] and determined the crystal structures of its apo-form and ternary complexes with CoA and four different aminoglycosides (gentamicin, sisomicin, neomycin, and paromomycin). These AAC(2')-Id structures unraveled the binding modes of different aminoglycosides, explaining the broad substrate specificity of the enzyme. Comparative structural analysis showed that the alpha4-helix and beta8-beta9 loop region undergo major conformational changes upon CoA and substrate binding. Additionally, structural comparison between the present paromomycin-bound AAC(2')-Id structure and the previously reported paromomycin-bound AAC(6')-Ib and 30S ribosome structures revealed the structural features of paromomycin that are responsible for its antibiotic activity and AAC binding. Taken together, these results provide useful information for designing AAC(2') inhibitors and for the chemical modification of aminoglycosides.
		+
		+	Structural and biochemical analyses of an aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis.,Jeong CS, Hwang J, Do H, Cha SS, Oh TJ, Kim HJ, Park HH, Lee JH Sci Rep. 2020 Dec 9;10(1):21503. doi: 10.1038/s41598-020-78699-z. PMID:33299080<ref>PMID:33299080</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7crm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mycolicibacterium smegmatis MC2 155]]
	[[Category: Do H]]		[[Category: Do H]]
	[[Category: Hwang J]]		[[Category: Hwang J]]
	[[Category: Jeong CS]]		[[Category: Jeong CS]]
	[[Category: Lee JH]]		[[Category: Lee JH]]

OCA at 07:29, 25 June 2021

OCA — Fri, 25 Jun 2021 07:29:57 GMT

←Older revision		Revision as of 07:29, 25 June 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7crm is ~~ON HOLD until Paper Publication~~	+	==Aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis-APO Structure==
-		+	<StructureSection load='7crm' size='340' side='right'caption='[[7crm]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CRM FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7crm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7crm OCA], [https://pdbe.org/7crm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7crm RCSB], [https://www.ebi.ac.uk/pdbsum/7crm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7crm ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Do H]]
		+	[[Category: Hwang J]]
		+	[[Category: Jeong CS]]
		+	[[Category: Lee JH]]

OCA at 05:26, 4 November 2020

OCA — Wed, 04 Nov 2020 05:26:31 GMT

←Older revision		Revision as of 05:26, 4 November 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7crm is ON HOLD	+	The entry 7crm is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7crm" [edit=sysop:move=sysop]

OCA — Wed, 19 Aug 2020 04:47:22 GMT

Protected "7crm" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:47, 19 August 2020

OCA: New page: '''Unreleased structure''' The entry 7crm is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 Aug 2020 04:47:20 GMT

New page: '''Unreleased structure''' The entry 7crm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7crm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7crm - Revision history

OCA at 16:14, 29 November 2023

OCA at 07:29, 25 June 2021

OCA at 05:26, 4 November 2020

OCA: Protected "7crm" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7crm is ON HOLD Authors: Description: Category: Unreleased Structures