OCA at 10:52, 27 March 2024

OCA — Wed, 27 Mar 2024 10:52:11 GMT

←Older revision		Revision as of 10:52, 27 March 2024
Line 1:		Line 1:

-	====	+	==Cryo-EM structure of Chikungunya virus in complex with Fab fragments of mAb CHK-124==
-	<StructureSection load='7cvy' size='340' side='right'caption='[[7cvy]]' scene=''>	+	<StructureSection load='7cvy' size='340' side='right'caption='[[7cvy]], [[Resolution\|resolution]] 5.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7cvy]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Chikungunya_virus Chikungunya virus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CVY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7cvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cvy OCA], [~~http~~://pdbe.org/7cvy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7cvy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7cvy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7cvy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 5.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cvy OCA], [https://pdbe.org/7cvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cvy RCSB], [https://www.ebi.ac.uk/pdbsum/7cvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cvy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/POLS_CHIKS POLS_CHIKS] Capsid protein possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. New virions attach to target cells, and after endocytosis their membrane fuses with the target cell membrane. This leads to the release of the nucleocapsid into the cytoplasm, followed by an uncoating event necessary for the genomic RNA to become accessible. The uncoating might be triggered by the interaction of capsid proteins with ribosomes. Binding of ribosomes would release the genomic RNA since the same region is genomic RNA-binding and ribosome-binding (By similarity). E3 protein's function is unknown (By similarity). E2 is responsible for viral attachment to target host cell, by binding to the cell receptor. Synthesized as a p62 precursor which is processed by furin at the cell membrane just before virion budding, giving rise to E2-E1 heterodimer. The p62-E1 heterodimer is stable, whereas E2-E1 is unstable and dissociate at low pH. p62 is processed at the last step, presumably to avoid E1 fusion activation before its final export to cell surface. E2 C-terminus contains a transitory transmembrane that would be disrupted by palmitoylation, resulting in reorientation of the C-terminal tail from lumenal to cytoplasmic side. This step is critical since E2 C-terminus is involved in budding by interacting with capsid proteins. This release of E2 C-terminus in cytoplasm occurs lately in protein export, and precludes premature assembly of particles at the endoplasmic reticulum membrane (By similarity). 6K is a constitutive membrane protein involved in virus glycoprotein processing, cell permeabilization, and the budding of viral particles. Disrupts the calcium homeostasis of the cell, probably at the endoplasmic reticulum level. This leads to cytoplasmic calcium elevation. Because of its lipophilic properties, the 6K protein is postulated to influence the selection of lipids that interact with the transmembrane domains of the glycoproteins, which, in turn, affects the deformability of the bilayer required for the extreme curvature that occurs as budding proceeds. Present in low amount in virions, about 3% compared to viral glycoproteins (By similarity). E1 is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 trimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion. Efficient fusion requires the presence of cholesterol and sphingolipid in the target membrane (By similarity).
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	*[[Virus coat proteins 3D structures\|Virus coat proteins 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chikungunya virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Diamond MS]]
		+	[[Category: Earnest JT]]
		+	[[Category: Fibriansah G]]
		+	[[Category: Fox JM]]
		+	[[Category: Kim AS]]
		+	[[Category: Kostyuchenko VA]]
		+	[[Category: Lok SM]]
		+	[[Category: Ng TS]]
		+	[[Category: Shu B]]
		+	[[Category: Zhou QF]]

OCA at 07:26, 4 November 2020

OCA — Wed, 04 Nov 2020 07:26:12 GMT

←Older revision		Revision as of 07:26, 4 November 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7cvy ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7cvy' size='340' side='right'caption='[[7cvy]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cvy OCA], [http://pdbe.org/7cvy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cvy RCSB], [http://www.ebi.ac.uk/pdbsum/7cvy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cvy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 06:51, 16 September 2020

OCA — Wed, 16 Sep 2020 06:51:18 GMT

←Older revision		Revision as of 06:51, 16 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7cvy is ON HOLD	+	The entry 7cvy is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7cvy" [edit=sysop:move=sysop]

OCA — Wed, 02 Sep 2020 05:17:28 GMT

Protected "7cvy" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 2 September 2020

OCA: New page: '''Unreleased structure''' The entry 7cvy is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Sep 2020 05:17:26 GMT

New page: '''Unreleased structure''' The entry 7cvy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7cvy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7cvy - Revision history

OCA at 10:52, 27 March 2024

OCA at 07:26, 4 November 2020

OCA at 06:51, 16 September 2020

OCA: Protected "7cvy" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7cvy is ON HOLD Authors: Description: Category: Unreleased Structures